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Molecular basis for the control of motor-based transport of MHC class II compartments Rocha, N.

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MHC class II compartments

Rocha, N.

Citation

Rocha, N. (2008, October 8). Molecular basis for the control of motor-based transport of MHC class II compartments. Retrieved from

https://hdl.handle.net/1887/13136

Version: Corrected Publisher’s Version

License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden

Downloaded from: https://hdl.handle.net/1887/13136

Note: To cite this publication please use the final published version (if

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Molecular basis for the control of motor-based transport of MHC class II compartments

Nuno Rocha

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Molecular basis for the control of motor-based transport of MHC class II compartments

Proefschrift

ter verkrijging van

de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. P.F. van der Heijden,

volgens besluit van het College voor Promoties te verdedigen op woensdag 8 oktober 2008

klokke 13.45 uur

door

Nuno Rocha

geboren te Lissabon, Portugal

in 1976

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Promotor: Prof. Dr. J.J. Neefjes

Referent: Prof. Dr. W.H. Moolenaar

Overige leden: Prof. Dr. J. Borst

Universiteit van Amsterdam

Prof. Dr. E.J. Wiertz Dr. N. Savage Dr. E. Roos

Het Nederlands Kanker Instituut, Amsterdam Prof. Dr. F. Koning

The work described in this thesis was performed at the Division of Tumor Biology of the Netherlands Cancer Institute, Amsterdam, The Netherlands, and supported by grants from the Netherlands Organization for Scientific Research (NWO), the Dutch Cancer Society (KWF), and a personal doctoral grant from the Portuguese Foundation for Science and Technology (FCT)/

Fundo Social Europeu (FSE)/ Programa Operacional Ciencia e Inovacao (POCI) 2010.

The publication of this thesis was financially supported by the Dutch Cancer Society (KWF), the Netherlands Organization for Scientific Research (NWO), and the Portuguese Ministry of Science, Technology and Higher Education/ Fundo Social Europeu (FSE)/ Programa Operacional

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Para os meus pais

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Contents

Chapter 1

Introduction:

Scope of the thesis 13

MHC class II molecules on the move for successful antigen presentation

EMBO J. 2008. 27:1-5

15

Chapter 2

Activation of endosomal dynein motors by stepwise assembly of Rab7-RILP-p150Glued, ORP1L, and the receptor bIII spectrin J. Cell Biol. 2007. 176:459-71

29

Chapter 3

The molecular mechanism of cholesterol control of late endosomal transport by dynein motors

Submitted. 2008

45

Chapter 4

A splice variant of RILP induces lysosomal clustering independent of dynein recruitment.

Biochem Biophys Res Commun. 2006. 344:747-56

79

Chapter 5

Rab7-RILP-ORP1L receptors couple late endosomal transport and fusion

In preparation

93

Chapter 6

Summary and Discussion 109

Nederlandse samenvatting

115

Curriculum Vitae

121

Appendix A Color figures

127

Referenties

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