Allogeneic haematopoietic stem cell donation and transplantation across the MHC class I barrier: "Faster is better than more. More is better than less".

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Allogeneic haematopoietic stem cell donation and transplantation

across the MHC class I barrier: "Faster is better than more. More is

better than less".

Heemskerk, M.B.A.

Citation

Heemskerk, M. B. A. (2006, September 28). Allogeneic haematopoietic stem cell donation and transplantation across the MHC class I barrier: "Faster is better than more. More is better than less". Retrieved from https://hdl.handle.net/1887/4578

Version: Corrected Publisher’s Version

License: Licence agreement concerning inclusion of doctoral thesis in the_{Institutional Repository of the University of Leiden} Downloaded from: https://hdl.handle.net/1887/4578

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4

The HLAMatchmaker algorithm is not a suitable

tool to predict the alloreactive cytotoxic T

lymphocyte response in vitro

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(4)

Abstract

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Introduction

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Material and Methods

Patient and donor selection

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Cytotoxic T-Lymphocyte Precursor Test

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Triplet mismatch analysis

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Results

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Figure 1. Number of triplet mismatches in patient/donor combinations from the donor registry of the Europdonor foundation with a single HLA-A or -B mismatch and a CTLp frequency  1 (left panel) or a CTLp frequency  10 (right panel) per 106_{PBL. Horizontal bars represent}

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Figure 2. Number of triplet mismatches in healthy responder/ stimulator combinations mismatched for at least one HLA class I antigen and a CTLp frequency  10 (left), a CTLp frequency between 11 and 100 (middle) or a CTLp frequency > 100 (right) per 106_{PBL. Horizontal bars represent the mean of the group.}

According to Mann-Whitney U test only the combinations with a &7/S IUHTXHQF\ ! VKRZHG D VLJQL¿FDQWO\ KLJKHU QXPEHU RI triplet mismatches compared with the combinations with a CTLp frequency between 11 and 100 (P=0.03) and a CTLp frequency  10 (P=0.04) per 106_PBL.

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69 HLAMatchmaker and CTL

Discussion

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71

HLAMatchmaker and CTL

References

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