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University of Groningen Omega transaminases: discovery, characterization and engineering Palacio, Cyntia Marcela

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University of Groningen

Omega transaminases: discovery, characterization and engineering

Palacio, Cyntia Marcela

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2019

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Palacio, C. M. (2019). Omega transaminases: discovery, characterization and engineering. Rijksuniversiteit Groningen.

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Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.

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Omega transaminases:

discovery, characterization

and engineering

Cyntia Marcela Palacio

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Cover design by Cyntia Palacio and Joris Goudsmits, adapted from a decorative mosaic artwork on the railway underpass on Moesstraat, Groningen.

Layout by Douwe Oppewal Printed by Ipskamp Drukkers B.V.

ISBN (printed version): 978-94-034-1559-8 ISBN (electronic version): 978-94-034-1558-1

The research described in this thesis was carried out in the ‘Groningen Biotechnology and Bimolecular Science Institute’ of the University of Groningen and was financially supported by EuroTANGO II (Project funded by the European Commission), by BIONEXGEN FP7, and by the BE-Basic Foundation.

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3

Omega transaminases:

discovery, characterization and

engineering

PhD thesis

to obtain the degree of PhD at the University of Groningen

on the authority of the Rector Magnificus Prof. E. Sterken

and in accordance with the decision by the College of Deans. This thesis will be defended in public on

Friday 26 April 2019 at 12.45 hours by

Cyntia Marcela Palacio

born on 11 August 1987 in Buenos Aires, Argentina

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4 Supervisor Prof. D.B. Janssen Assessment committee Prof. P. Berglund Prof. D.J. Slotboom Prof. G.J.W. Euverink

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5

TABLE OF CONTENTS

Chapter 1: Introduction

7

Chapter 2: Characterization of the caprolactam degradation

39

pathway in Pseudomonas jessenii using

mass spectrometry-based proteomics

Chapter 3: Biochemical properties of a Pseudomonas

63

aminotransferase involved in caprolactam metabolism

Chapter 4: Enhancing thermal stability of Pseudomonas jessenii 87

transaminase by computational library design

Chapter 5: Enzymatic network for production of ether amines

113

from alcohols

Chapter 6: Summary and perspectives

133

Chapter 7: Samenvatting en perspectief

145

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