University of Groningen
Omega transaminases: discovery, characterization and engineering
Palacio, Cyntia Marcela
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Publication date: 2019
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Palacio, C. M. (2019). Omega transaminases: discovery, characterization and engineering. Rijksuniversiteit Groningen.
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PROPOSITIONS
belonging to the thesis
Omega transaminases:
discovery, characterization and engineering
1. Samples from polluted environments are a powerful source for finding novel transaminases through classical microbial enrichment (Chapter 2)
2. The complexity of assembling artificial enzymatic networks and cascades shows us the exquisite and delicate design of nature’s metabolic pathways (Chapter 5)
3. Computational design is an effective strategy for obtaining stabilized transaminases required for industrial use but requires careful selection of target regions (Chapter 4) 4. The role of P. jessenii transaminase in caprolactam degradation and the high activity
of this enzyme and its homologs with aromatic substrates illustrate that transaminase biological function cannot be derived from the specificity profile (Chapter 3)
5. In comparison to the highly sterile protocols for protein purification used in the pharmaceutical industry, protein isolation in an academic setting is an extremely dirty process
6. In a PhD project there is an inverse relationship between learning and perceived knowledge
7. A PhD project is not just about scientific experiments, it also requires serious negotiations
