University of Groningen
Synthesis and characterization of lactose and lactulose derived oligosaccharides by
glucansucrase and trans-sialidase enzymes
Pham, Thi Thu Hien
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Publication date: 2018
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Pham, T. T. H. (2018). Synthesis and characterization of lactose and lactulose derived oligosaccharides by glucansucrase and trans-sialidase enzymes. University of Groningen.
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Synthesis and characterization of
lactose and lactulose derived oligosaccharides by
glucansucrase and trans-sialidase enzymes
Cover designed by Hien Pham and Johan Schabbink Printed by: Ipskamp Drukkers, Enschede
ISBN printed: 978-94-034-1186-6 ISBN digital: 978-94-034-1187-3
The work described in this thesis was carried out in the Microbial Physiology Group of the Groningen Biomolecular Sciences and Biotechnology Institute at the University of Groningen and was financially supported by the University of Groningen/Campus Fryslân, FrieslandCampina and The University of Groningen.
Synthesis and characterization of
lactose and lactulose derived oligosaccharides by
glucansucrase and trans-sialidase enzymes
PhD Thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Supervisor
Prof. L. Dijkhuizen
Co-supervisor
Dr. S. S. van Leeuwen
Assessment Committee
Prof. G.J.P.H. Boons
Prof. O.P. Kuipers
Prof. M.W. Fraaije
Table of Contents
Chapter 1 Introduction 7
Chapter 2 Structural characterization of glucosylated lactose
derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes
35
Chapter 3 Stimulatory effects of novel glucosylated lactose
derivatives GL34 on growth of selected gut bacteria
61
Chapter 4 Mutational analysis of the role of the glucansucrase
Gtf180-ΔN active site residues in product and linkage specificity with lactose as acceptor substrate
93
Chapter 5 Structural characterization of glucosylated GOS
derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes
129
Chapter 6 Synthesis and characterization of sialylated lactose and
lactulose derived oligosaccharides by Trypanosoma
cruzi trans-sialidase
