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University of Groningen

Metabolic capabilities of Lactococcus lactis

Hernandez-Valdes, Jhonatan

DOI:

10.33612/diss.130772158

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2020

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Hernandez-Valdes, J. (2020). Metabolic capabilities of Lactococcus lactis: Flavor, amino acids and phenotypic heterogeneity. University of Groningen. https://doi.org/10.33612/diss.130772158

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nederlandse samenvatting

¡Muchas gracias! I have had a great time during my PhD. This adventure was ama-zing due to the support of many people. Here, I want to express my gratitude to all of you.

Specially, I am very thankful to my supervisor, Oscar Kuipers. I enjoyed

very much to work with you. Your pre-sence makes MolGen an exciting, fun, and inspiring place to be. But not only at work, going to conferences with you and enjoying some agua de Valencia together was fun. I appreciate your guidance and wise advice on how to become a Scientist. A big thank you for allowing me to develop my own research projects, for supporting me and for not stopping me. More than giving me freedom, you promoted my creativity and guided me.

I am also very grateful to Jan Kok.

Thanks for sharing your knowledge about the famous bacterium Lactoccoccus

lac-tis. Getting advices from you is always

inspiring, and certainly you helped me

to find the origins of uninvestigated and old L. lactis strains.

My PhD was possible due to the work with Herwig Bachmann and Bas Teu-sink. Together with Rinke van Taten-hove, thank you for your advices and

feedback during our regular meetings. I am very happy about the results we obtained during the development of our microdroplets project.

Thanks to Andrius Buivydas, my first

supervisor at MolGen during my master internship, for believing in me and sup-porting me to pursue a PhD at MolGen. Likewise, thanks to Ana Solopova, my

daily supervisor at the beginning of my PhD, for helping me and motivate me to work with L. lactis metabolism. I enjoyed sharing fun moments with both of you at conferences and visiting you in Cork. Querido Manolo, gracias también a

ti por hacerme sentir parte de MolGen desde el principio, hace ya varios años. Qué divertido ha sido compartir tiempo,

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acknowledgements acknowledgements

por tanto, nuestra amistad comenzó en México, pero aquí nuestra amistad ha crecido bastante, compartimos casi todo, ¡desde la casa hasta la oficina!. Gracias por siempre estar presente, escucharme, nuestros paseos, platicas, cenas, fiestas y mucho más. Te quiero mucho. Lu and Paty, thank you so much.

It has been a pleasure to share fun moments with friends at MolGen. Elrike,

we had a great time as iGEM supervisors and shared fun moments, and thanks for your advices and also believing in me since the beginning. Mark, you are one

of my first friends in Groningen, and have contributed to make my PhD a great adventure, thank you. Gracias a ti Carlos,

aunque fisicamente viviste solo unos cuantos meses en Groningen, me alegra que siempre estas presente, seguimos visitandonos y has contribuido a que disfrutara tanto mi tiempo de doctorado. To all the students that worked with me, thanks for your help and teaching me how to become better as a supervisor: The iGEM team 2016, Peter, Amulya, Juan, Max and Myrthe.

A big thank to the current and past MolGeners I have met during the last four years. Fleur, it is always so much

fun to dance with you. Many thanks to

Barbara, Mimi, Luiza, Chenxi, Ruben, Auke, Chunxue, Amanda, Diego, and Vicky for all the nice chats, parties,

ad-vises, scientific discussions. conferencias, cenas, paseos, orujo y planes

tranqui. Gracias por ser un buen amigo y contagiarme con tu chispa, alegría y buen humor.

Jordi thank you very much for your

help to finish the heterogeneity project. And importantly, my scientific writing

skills improved so much thanks to you. I want to also thank to my co-authors:

Jos Hermans, Marcel de Vries and Johan Teunis. I could have not finished

my projects without your help; it was a pleasure to work with you.

Anne de Jong, thank you so much

for your help during the transcriptomics courses and genome sequencing data. I remember that after just fifteen days of starting my PhD we were already working in a transcriptomics course.

Harma and Siger, thanks a lot for all

the things you do to keep the labs working. Many thanks to Manon and Jannet, for

helping us with all the administration, bookings, and the nice chitchatting we had. Peter, Anna and Jesse, thanks a

lot for helping us to make our work at the lab easier.

Next, I want to express my gratitude to my paranymphs and friends Lu and Paty.

I enjoyed very much to share the office with both of you. Lu, thanks for sharing

fun moments and scientific talks, making me laugh so much, singing together Gaga songs, and also introducing me to

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acknowledgements acknowledgements

your friendship: Ahmed, Alessandra, Alicia, Ash, Abhishek, Ele, Fernando, Alejandro, Giacomo, Suzana, Lucy, Tomas, and Chiara.

Erika, mi pareja de baile,

comparti-mos más risas que pasos de baile en esas clases. Gracias por compartir tu alegría conmigo, y me alegra verte contenta con tu familia, David y el pequeño Thiago.

Mi querida Alma, qué alegría me da

que estás ¨cerca¨, gracias por compartir tanto conmigo, me encanta tener paseos contigo y que nuestra amistad es tan bonita. También que alegría contar con su amistad Manu, Pau, Felipe y Moni,

amigos desde la facultad de Química y qué genial poder verlos por aquí en Europa o en las posadas en México.

Thanks a lot to my lovely Yasemin

and Anastasia. Our friendship began

in that small town, Newark in USA, and fortunately afterwards we became neigh-bors, you both living in Germany. So many adventures together, trips, parties, aperol spritz, and so much fun.

Joana and Paolo, my dear friends, I

am so happy to have you around. Thanks for helping to make this PhD a great experience, introducing me to my favo-rite game ticket to ride, and good luck with finishing your projects, and looking forward to celebrate with you the end of your PhD.

Mi parce Andrea, llegaste a

Gronin-gen un par de meses, suficiente para It was a pleasure to share the big L.

lactis lab with a lot of people. We had the

best music, thanks to Jakob, Qian, Jingqi, Yuxin, Fangfang, Xinghong, Jingjing, Longcheng, and Yushan. Wish you good

luck with your PhD projects, including to

Anne-Marie, Anna, Zhibo, Meishan, Yunhai, Ilaria and Tycho.

Mis queridos Bosque friends Clau, Yayo y Pam, muchísimas gracias por su

apoyo, qué alegría compartir aventuras, fiestas, cenas, platicas y más. Qué suerte que Clau y Yayo llegaron a MolGen,

des-pués adoptamos a Pam del grupo vecino,

y nuestra amistad sigue creciendo. Aquí seguiré para apoyarlos, espero verlos pronto también terminando el doctorado y celebrando con ustedes. Gracias por su amistad sincera, los adoro.

To my dear Simone and Brankica, my

friends and roomies, it was such a nice experience to share so much with you. Thanks for your support always, I am so happy that our friendship started in Groningen, and seeing you again makes me very happy.

Thank you Jesse for introducing me to

the Dutch culture, but more importantly for your sincere friendship and support.

Loek, amiguis, thank you so much

for sharing great moments with me and your friendship, your positive vibe and energy is contagious.

I am grateful to have met you here in Groningen, thanks for so much fun and

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acknowledgements

Gracias a mis amigos por todo su apoyo, me encanta verlos cada que se puede: Anita, Cris, Héctor, Jo, Carla y Carlitos, David, Oscar, Jocelyn, Sergio, Edu, Sergio, Pam, Irene, Jair, Alexis, Ale Pérez, Laura Soto, Dancer, Daniel, Mirna, Karen Pérez, Selene.

It is not easy to star a new life in a different country. Muchas gracias a mi familia en mi querido México por apoyarme en la decisión de hacer un doctorado en Holanda. Mamá, papá,

hermanito Yair, muchas gracias por

su apoyo y cariño, gracias a ustedes estoy concluyendo este sueño. Llega-ron Karen y Regina a la familia, más

alegría para la familia. Muchísimas gracias a toda mi familia, mi yapa, tías, tíos, primos, primas. Los amo

y gracias por tanto.

And of course, to my dear Dennis, thank

you so much for all the nice days since I met you until now. We are collecting so many memories and adventures together. We make a great team, and having you next to me makes me so happy. Without forgetting your contribution to science by shaking some tubes in the lab for me a couple of times. Love you so much. Ook wil ik mijn schoonfamilie bedanken voor

het warme welkom en de interesse die ze toonden in mijn onderzoek.

convertirte en un buen amigo. Cumplí mi promesa de ir a tu pueblo y wow qué bonito es Trieste. Muchísimas gracias por estar presente.

Joop, my local friend in Groningen. Heel

erg bedankt voor het helpen om beter Neder-lands te spreken en om met mij te oefenen, de gezelligheid, alle leuke momenten, ook met Jet. En ik hoop dat ik in de loop der tijd

nog beter Nederlands kan spreken zodat ik nog meer met je kan praten.

A pesar de estar tan lejos, me siento muy afortunado por haber recibido el apoyo de muchas personas. Mi querida

Karen, muchas gracias por todo tu apoyo,

ir a visitarme en todas partes, aventuras y amistad. Mi querida Lily, a pesar de

que estas tan lejos, rodeada de koalas y canguros, seguimos cultivando nuestra amistad virtualmente o con cartas. Querido amigo Frank, gracias por estar presente,

qué alegría que estuviste hace poco de visita. Liss y Maritere, intensas, qué

felicidad tenerlas conmigo desde hace ya muchos años, qué jóvenes éramos cuando empezamos la licenciatura y qué nuestra amistad siga creciendo.

Querido Omar, gracias por tu amistad

y por estar presente en mi vida. Además quiero resaltar el gran talento que tienes, gracias por aceptar ayudarme con el diseño de esta tesis.

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