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University of Groningen

Toxic love

Rouhana, Jessy

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2019

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Rouhana, J. (2019). Toxic love: Evolutionary genomics of the enigmatic Sex Peptide. University of Groningen.

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Toxic Love

Evolutionary genomics of the

enigmatic Sex Peptide

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Cover design: Gaetan Menanteau

Layout: Jessy Rouhana

Printed by: Gildeprint, Enschede

ISBN (printed): 978-94-034-1170-5

ISBN (digital): 978-94-034-1169-9

This research has been carried out at the Groningen Institute for Evolutionary Life Sciences (GELIFES) of the University of Groningen (The Netherlands) and at the School of Biology of the university of East Anglia (United Kingdom), according to the requirements of the Graduate School of Science (Faculty of Science and Engineering, University of Groningen).

This research was funded by Ubbo Emmius PhD scholarship from the university of Groningen, a PhD scholarship from the university of East Anglia, grants from the John and Pamela Salters charitable trust and the Leverhulme Trust.

© This copy of the thesis has been supplied on condition that anyone who consults it is understood to recognise that its copyright rests with the author and that use of any information derived there from must be in accordance with current UK Copyright Law. In addition, any quotation or extract must include full attribution.

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Toxic Love

Evolutionary genomics of the enigmatic Sex Peptide

PhD thesis

to obtain the degree of PhD of the University of Groningen

on the authority of the Rector Magnificus Prof. E. Sterken

and in accordance with the decision by the College of Deans.

and

to obtain the degree of PhD of the University of East Anglia on the authority of the Vice Chancellor Prof. D. Richardson and in the accordance with the decision by the Science Faculty and Associate

Dean for Post Graduate Studies Prof A. Grant. Double PhD degree

This thesis will be defended in public on Monday 07 January 2018 at 11:00

By

Jessy Rouhana

born on 02 July 1989 In Bikfaya, Lebanon

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Supervisors

Prof. B. Wertheim

Prof. T. Chapman

Assessment committee

Prof. J.C. Billeter

Prof. D. Richardson

Prof. M. Wolfner

Prof. C. Wilson

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Table of contents

Chapter 1

General introduction

7

Chapter 2

A novel immuno-Quantitative PCR assay for quantifying Sex

Peptide protein in Drosophila melanogaster

37

Chapter 3

Genetic variation in immune responses to Sex Peptide,

Drosophila melanogaster

73

Chapter 4

Sex peptide “master regulator” of female Receptivity, Egg

laying and Longevity in Drosophila melanogaster

119

Chapter 5

General discussion

177

Bibliography

193

Summary

225

Samenvatting

229

Personal information

235

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