Cover Page
The handle
http://hdl.handle.net/1887/136915
holds various files of this Leiden
University dissertation.
Author: Voorneveld, P.W.
Appendix: List of abbreviations
List of abbreviations
5-FU Fluorouracil
AKT (PKB) Protein Kinase B
APC Adenomatous Polyposis Coli
ATP Adenosine Triphosphate
BCIP 5-Bromo-4-chloro-3-indolyl phosphate p-toluide salt
BMP Bone Morphogenetic Protein
BMPR Bone Morphogenetic Protein Receptor
BRDU 5-BRomo-2'-DeoxyUridine
BRE BMP Responsive Element
BSA Bovine Serum Albumin
CAG Chicken Beta-actin Promotor
CAII Carbonic Anhydrase II
CD1 Cluster of Differentation 1
CDH1 gene coding for Ecadherin
CIMP CpG Island Methylator Phenotype
CMFDA 5-ChloroMethylFluorescein DiAcetate
CRC Colorectal Cancer
CSC Cancer Stem Cells
CTNNB1 gene coding for β-catenin
CYP Cytochrome P450
DAB Diaminobenzidine
DCC Deleted in Colorectal Cancer
DMSO Dimethyl sulfoxide
DNA Deoxyribonucleic Acid
EDTA EthyleneDiamineTetraacetic Acid
EGTA EthyleneGlycol-bis(2-aminoethylether)-N,N,N',N'-Tetraacetic Acid EMT Epithelial-to-Mesenchymal Transition
ERK Extracellular Signal-Regulated Kinase
FCS Fetal Calf Serum
FEG Field Emission Gun
G418 Geneticin
GAPDH Glyceraldehyde 3-phosphate dehydrogenase
GFP Green Fluorescent Protein
GREM1 Gremlin1
GSK Glycogen Synthese Kinase
HA-tag HemAgglutinin Tag
HRP HorseRadish Peroxidase
IC50 half maximal inhibitory concentration
ip intraperitoneal injection
Appendix: List of abbreviations
ITO Indium Tin Oxide
JNK c-Jun N-Terminal kinase
JP Juvenile Polyposis
LacZ gene coding for Beta-galactosidase
LIMK LIM Kinase
LUC LUCiferase
MAPK Mitogen-Activated Protein Kinase MKK Mitogen-Activated Protein Kinase Kinase
mRNA messengerRNA
NaCl NatriumChloride
NBT Nitro Blue Tetrazolium
OLFM4 OLFactoMedin 4
PBS Phosphate Buffered Saline
pcDNA protamine complementary DNA
PCR Polymerase Chain Reaction
qPCR quantative PCR
RNA RiboNucleic Acid
ROCK Rho-associated Protein Kinase
SD Standard Deviation
SDS Sodium Dodecyl Sulfate
SEM Scanning Electron Microscope
SEM Standard Error of the Mean
shRNA short hairpin RNA
SIP1 SMN Interacting Protein 1
siRNA small interfering RNA
SMAD Small MOthers against Decapentaplegic4
SNP Single Nucleotide Polymorphism
SSC Saline Sodium Citrate
TBS Tris Buffered Saline
TENG-T 10 mM Tris, 5 mM EDTA, 0.15 mM NaCl, 0.25% gelatin, 0.05% Tween 20
TGFβ Transforming Growth Factor-β
TMA Tissue Microarray
VIM gene coding for Vimentin
Appendix: Contributing authors
Contributing authors
Listed alphabeticallyEsther Bastiaannet
Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands Department of Gerontology & Geriatrics, Leiden University Medical Center, the Netherlands
Izak Biemond
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Gijs van den Brink
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands; Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, the Netherlands; Tytgat Institute for Liver & Intestinal Research, Academic Medical Center, Amsterdam, the Netherlands
Peter ten Dijke
Department of Molecular Cell Biology, Cancer Genomics Centre, Leiden University Medical Center, Leiden, the Netherlands
Ronald van Eijk
Department of Pathology, Leiden University Medical Center, Leiden, the Netherlands
Daan Geraets
DDL Diagnostic Laboratory, Rijswijk, the Netherlands
James Hardwick
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Lucas Hawinkels
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands; Department of Molecular Cell Biology, Cancer Genomics Centre, Leiden University Medical Center, Leiden, the Netherlands
Ron Herings
PHARMO Institute for Drug Outcomes Research, the Netherlands
ITO Indium Tin Oxide
JNK c-Jun N-Terminal kinase
JP Juvenile Polyposis
LacZ gene coding for Beta-galactosidase
LIMK LIM Kinase
LUC LUCiferase
MAPK Mitogen-Activated Protein Kinase MKK Mitogen-Activated Protein Kinase Kinase
mRNA messengerRNA
NaCl NatriumChloride
NBT Nitro Blue Tetrazolium
OLFM4 OLFactoMedin 4
PBS Phosphate Buffered Saline
pcDNA protamine complementary DNA
PCR Polymerase Chain Reaction
qPCR quantative PCR
RNA RiboNucleic Acid
ROCK Rho-associated Protein Kinase
SD Standard Deviation
SDS Sodium Dodecyl Sulfate
SEM Scanning Electron Microscope
SEM Standard Error of the Mean
shRNA short hairpin RNA
SIP1 SMN Interacting Protein 1
siRNA small interfering RNA
SMAD Small MOthers against Decapentaplegic4
SNP Single Nucleotide Polymorphism
SSC Saline Sodium Citrate
TBS Tris Buffered Saline
TENG-T 10 mM Tris, 5 mM EDTA, 0.15 mM NaCl, 0.25% gelatin, 0.05% Tween 20
TGFβ Transforming Growth Factor-β
TMA Tissue Microarray
VIM gene coding for Vimentin
Appendix: Contributing authors
Myrthe van Herk-Sukel
PHARMO Institute for Drug Outcomes Research, the Netherlands
Daniël Hommes
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Jacob Hoogenboom
Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands
Rutger Jacobs
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Liudmila Kodach
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Kerem Sebib Korkmaz
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Peter Kuppen
Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands
Suzanne Lam
Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, the Netherlands
Gerrit-Jan Liefers
Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands
Anne Liesker
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Nalan Liv
Appendix: Contributing authors
Wilma Mesker
Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands
Noël de Miranda
Department of Pathology, Leiden University Medical Center, Leiden, the Netherlands
Ilse Molendijk
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Hans Morreau
Department of Pathology, Leiden University Medical Center, Leiden, the Netherlands
Carel J. van Noesel
Department of Pathology, Academic Medical Center, Amsterdam, the Netherlands
Johan Offerhaus
Department of Pathology, University Medical Center, Utrecht, the Netherlands
Madelon Paauwe
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands; Department of Molecular Cell Biology, Cancer Genomics Centre, Leiden University Medical Center, Leiden, the Netherlands
Gabi van Pelt
Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands
Maikel Peppelenbosch
Department of Gastroenterology & Hepatology, Erasmus MC, Erasmus University Rotterdam, Rotterdam, the Netherlands
Marlies Reimers
Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands
Karien de Rooij
Department of Radiology, Leiden University Medical Center, Leiden, the Netherlands Percuros B.V., Leiden, the Netherlands
Sanne Rozekrans
Appendix: Contributing authors
Antoine Sitters
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Erwin Smolders
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Vanessa Stache
Max Delbrück Center for Molecular Medicine, Berlin, Germany
Rob Tollenaar
Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands
Cornelis van de Velde
Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands
Hein Verspaget
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Thijs de Vos
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Tom van Wezel
Department of Pathology, Leiden University Medical Center, Leiden, the Netherlands
Sophie de Wit
Department of Gastroenterology, Leiden University Medical Center, Leiden, the Netherlands
Marjolein Zanders
Comprehensive Cancer Centre the Netherlands
Christiaan Zonnevylle
Appendix: Presentations & Publications
Presentations
Poster presentationsDigestive Disease Week, May 2015, Washington DC, USA; Statin Use After Diagnosis
Improves Survival in Colorectal Cancer Patients
Digestive Disease Week, May 3-6 2014, Chicago, IL, USA; Fibroblasts promote invasion
in SMAD4 negative colorectal cancers by producing BMP-2
United European Gastroenterology Week, October, 2013, Berlin, Germany; The
Bone Morphogenetic Protein Pathway either enhances or inhibits the Wnt pathway depending on SMAD4 and p53 status in colorectal cancer.
Digestive Disease Week, May 18-21, 2013, Orlando, Fl, USA; Loss of Bone
Morphogenetic Protein Receptor IA predicts poor survival in patients with pancreatic cancer and is responsible for invasiveness and proliferation of cancer cells.
United European Gastroenterology Week, October 20-24, 2012, Amsterdam, the Netherlands; Loss of Bone Morphogenetic Protein Receptor 1a predicts poor survival
in patients with pancreatic cancer and is responsible for invasiveness and proliferation of cancer cells.
Digestive Disease Week, May 7-10, 2011, Chicago, IL, USA; Non-Canonical Bone
Morphogenetic Protein Signaling induces Epithelial to Mesenchymal transition in SMAD4 deficient colorectal cancers.
BMP congress, September 15-18 2010, Leuven, Belgium; The Role of Bone
Morphogenetic Protein Signaling and Transforming Growth Factor β Signaling and their Components in Colorectal Cancer.
Oral presentations
Digestive Disease Week, May 3-6, 2014, Chicago, IL, USA; Conditional activation of
BMP signalling leads to loss of the stem cell compartment.
Spring meeting Dutch Gastroenterology Society (NVGE), March 2014, Veldhoven, the Netherlands; Fibroblasts promote invasion in SMAD4 negative colorectal cancers by
producing BMP-2
Spring meeting Dutch Gastroenterology Society (NVGE), March 2014, Veldhoven, the Netherlands; Conditional activation of BMP signalling leads to loss of the stem cell
Appendix: Presentations & Publications
Digestive Disease Week, May 18-21, 2013, Orlando, Fl, USA; The Bone Morphogenetic
Protein Pathway either enhances or inhibits the Wnt pathway depending on SMAD4 and p53 status in colorectal cancer.
Spring meeting Dutch Gastroenterology Society (NVGE), March 22, 2013, Veldhoven, the Netherlands; The Bone Morphogenetic Protein Pathway either enhances or inhibits
the Wnt pathway depending on SMAD4 and p53 status in colorectal cancer.
Spring meeting Dutch Gastroenterology Society (NVGE), March 22, 2012, Veldhoven, the Netherlands; Loss of Bone Morphogenetic Protein Receptor IA predicts poor
survival in patients with pancreatic cancer and is responsible for invasiveness and proliferation of cancer cells.
United European Gastroenterology Week, October 20-24, 2012, Amsterdam, the Netherlands; SMAD4-independent Bone Morphogenetic Protein signalling is
associated with a poor prognosis in colorectal cancer patients.
Digestive Disease Week, May 19-22, 2012, San Diego, CA, USA; Rock-inhibitors reduce
the induction of Epithelial-to-Mesenchymal Transition, migration and invasion in SMAD4 deficient colorectal cancers caused by Bone Morphogentic Protein Signalling.
Spring meeting Dutch Gastroenterology Society (NVGE), March 22, 2012, Veldhoven, the Netherlands; Rock-inhibitors reduce the induction of Epithelial-to-Mesenchymal
Transition, migration and invasionin SMAD4 deficient colorectal cancers caused by Bone Morphogentic Protein Signalling.
United European Gastroenterology Week, October 22-26, 2012, Stockholm, Sweden;
Bone Morphogenetic Protein Signaling induces epithelial to mesenchymal transition in SMAD4 deficient colorectal cancers.
Spring meeting Dutch Gastroenterology Society (NVGE), March 17, 2011, Veldhoven, the Netherlands; Non-canonical Bone Morphogenetic Protein Signaling induces
Appendix: Presentations & Publications
Publications
Voorneveld PW, Kodach LL, Rozekrans SL, Jacobs RJ, van den Brink GR, Hardwick JCH. Conditional Activation of BMP Signalling Leads to Loss of the Stem Cell Compartment.
Manuscript in preperation
Voorneveld PW, Ouahoud S, van der Burg LRA, de Jonge-Muller ESM, Schoonderwoerd MJA, Paauwe M, de Vos T, de Wit S, van Pelt GW, Mesker WE, Hawinkels LJAC, Hardwick JCH. Bidirectional tumor/stroma crosstalk promotes metastasis in mesenchymal colorectal cancer. Oncogene. 2020 Jan 23. doi: 10.1038/s41388-020-1157-z. Voorneveld PW, Reimers MS, Bastiaannet E, Jacobs RJ, van Eijk R, Zanders MMJ, Herings RMC, van Herk-Sukel MPP, Kodach LL, van Wezel T, Kuppen PJK, Morreau H, van de Velde CJH, Hardwick JCH, Liefers GJ. Statin Use After Diagnosis Improves Survival in Colorectal Cancer Patients. Gastroenterology. 2017 Aug;153(2):470-479. e4. doi: 10.1053/j.gastro.2017.05.011.
Burggraaf J, Kamerling IM, Gordon PB, Schrier L, de Kam ML, Kales AJ, Bendiksen R, Indrevoll B, Bjerke RM, Moestue SA, Yazdanfar S, Langers AM, Swaerd-Nordmo M, Torheim G, Warren MV, Morreau H, Voorneveld PW, Buckle T, van Leeuwen FW, Ødegårdstuen LI, Dalsgaard GT, Healey A, Hardwick JC. Detection of colorectal polyps in humans using an intravenously administered fluorescent peptide targeted against c-Met. Nat Med. 2015 Aug;21(8):955-61. doi: 10.1038/nm.3641. Epub 2015 Jul 13. Voorneveld PW, Jacobs RJ, Kodach LL, Hardwick JCH. A Meta-Analysis of SMAD4 Immunohistochemistry as a Prognostic Marker in Colorectal Cancer
Transl Oncol. 2015 Feb;8(1):18-24. doi: 10.1016/j.tranon.2014.11.003.
Voorneveld PW, Jacobs RJ, Liv N, Hoogendoorn JP, Zonnevylle C, Biemond I, Hommes DW, de Rooij K, van Noesel CJ, Offerhaus GJ, van den Brink GR, Peppelenbosch M, ten Dijke P, Kodach LL and Hardwick JCH. Loss of SMAD4 switches BMP signalling from tumour suppressive into pro-metastatic via activation of Rho-Rock. Gastroenterology.
2014 Jul;147(1):196-208.e13. doi: 10.1053/j.gastro.2014.03.052. Epub 2014 Apr 3
Voorneveld PW, KodachLL, JacobsRJ, van NoeselCJM, PeppelenboschMP, Sebib KorkmazK, MolendijkI, DekkerE, MorreauH, van Pelt G, MeskerWE, GeraetsDT, HommesDW, Offerhaus GJA, Verspaget HW, van den BrinkGR, ten DijkeP and James C.H. Hardwick. The Bone Morphogenetic Protein Pathway either enhances or inhibits the Wnt pathway depending on the SMAD4 and p53 status in colorectal cancer. Br J
Appendix: Presentations & Publications
Voorneveld PW, Stache V, Jacobs RJ, Smolders E, Sitters AI, LieskerA, Sebib Korkmaz K, Lam SM, De Miranda NFCC, Morreau H, Kodach LL & Hardwick JCH. Reduced Bone Morphogenetic Protein Receptor IA expression predicts poor survival in patients with pancreatic cancer and is responsible for invasive phenotype of cancer cells. Br J
Cancer. 2013 Oct 1;109(7):1805-12. doi: 10.1038/bjc.2013.486. Epub 2013 Aug 22
Sebib Korkmaz K, de Rooij BF, van Hoek B, Janse M, Coenraad MJ, van der Reijden JJ, Weersma RK, Porte RJ, Voorneveld PW, Baranski AG, Verspaget HW. MMP-2 is a disease-modifying gene in primary sclerosing cholangitis. Liver Int. 2013 Jun 4. doi:
10.1111/liv.12237.
Voorneveld PW, Jacobs RJ, De Miranda NF, Morreau H, van Noesel CJ, Offerhaus GJ, Kodach LL, Hardwick JC. Evaluation of the prognostic value of pSMAD immunohistochemistry in colorectal cancer. Eur J Cancer Prev. 2013 Feb 17.
Liv N, Zonnevylle AC, Narvaez AC, Effting AP, Voorneveld PW, Lucas MS, Hardwick JC, Wepf RA, Kruit P, Hoogenboom JP. Simultaneous correlative scanning electron and high-NA fluorescence microscopy. PLoS One. 2013;8(2):e55707. doi: 10.1371/journal.
pone.0055707.
Jacobs RJ, Voorneveld PW, Kodach LL, Hardwick JCh. Cholesterol metabolism and colorectal cancers. Curr Opin Pharmacol. 2012 Dec;12(6):690-5.
Baan B, Dihal AA, Hoff E, Bos CL, Voorneveld PW, Koelink PJ, Wildenberg ME, Muncan V, Heijmans J, Verspaget HW, Richel DJ, Hardwick JC, Hommes DW, Peppelenbosch MP, van den Brink GR. 5-aminosalicylic acid inhibits cell cycle progression in a phospholipase D dependent manner in colorectal cancer. Gut. 2012
Dec;61(12):1708-15.
