The handle
http://hdl.handle.net/1887/80612
holds various files of this Leiden University
dissertation.
Author: Mieremet, A.
Title: A multidirectional approach to optimize morphogenesis and barrier characteristics
of human skin equivalents
A multidirectional approach to optimize morphogenesis and
barrier characteristics of human skin equivalents
Dermatology of the Leiden University Medical Center (LUMC) and at the Division BioTherapeutics of the Leiden Academic Centre for Drug Research (LACDR), Leiden University (Leiden, The Netherlands). The research was financially supported by the Netherlands Organization for Scientific Research (NWO) domain Applied and Engineering Sciences (TTW) (project number 13151), which is partly funded by the Ministry of Economic Affairs and Climate Policy, and the Ministry of Education, Culture and Science.
A multidirectional approach to optimize morphogenesis and
barrier characteristics of human skin equivalents
Proefschrift
Ter verkrijging van de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op donderdag 14 november 2019
klokke 11.15 uur
door
Arnout Mieremet
Promotiecommissie:
Prof. dr. H. Irth, Leiden Academic Centre for Drug Research (LACDR) (Chairman) Prof. dr. A.P. IJzerman, Leiden Academic Centre for Drug Research (LACDR) (Secretary)
Prof. dr. M.H. Vermeer, Leiden University Medical Center (LUMC) Prof. dr. M. Schäfer-Korting, Free University Berlin
Contents
Chapter 1 Introduction 7
Part I
Chapter 2 Recapitulation of native dermal tissue in a
full-thickness human skin model using human collagens 33 Chapter 3 Improved epidermal barrier formation in human skin
models by chitosan modulated dermal matrices
57
Part II
Chapter 4 Characterization of human skin equivalents developed at body’s core and surface temperatures
89 Chapter 5 Human skin equivalents cultured under hypoxia
display enhanced epidermal morphogenesis and lipid barrier formation
113 Chapter 6 Unravelling effects of relative humidity on lipid
barrier formation in human skin equivalents 139 Chapter 7 Shedding light on the effects of 1,25-dihydroxyvitamin
D3 on epidermal lipid barrier formation in three-dimensional human skin equivalents
165
Part III
Chapter 8 Contribution of palmitic acid to epidermal
morphogenesis and lipid barrier formation in human skin equivalents
193 Chapter 9 Multitargeted approach for the optimization of
morphogenesis and barrier formation in human skin equivalents
223
Chapter 10 Summary and perspectives 251
Chapter 11 Nederlandstalige samenvatting (Dutch Summary) 270
Curriculum Vitae 280
