Understanding doublecortin-like kinase gene function through transgenesis
Schenk, G.J.
Citation
Schenk, G. J. (2010, October 21). Understanding doublecortin-like kinase gene function through transgenesis. Retrieved from https://hdl.handle.net/1887/16066
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden Downloaded from: https://hdl.handle.net/1887/16066
Note: To cite this publication please use the final published version (if applicable).
Understanding Doublecortin-Like Kinase Gene Function Through
Transgenesis
Geert J. Schenk
2
Geert J. Schenk
Understanding Doublecortin-Like Kinase Gene Function Through Transgenesis Thesis, Leiden University
Date: September 2010 ISBN: 978-90-8891-195-8 Cover: © Antonio Hoogervorst
Published by: Uitgeverij BOXPress, Oisterwijk
Printed by: Proefschriftmaken.nl || Printyourthesis.com
© Geert J. Schenk
No parts of this thesis may be reproduced or transmitted in any form or by any means without written permission of the author.
3
Understanding Doublecortin-Like Kinase Gene Function Through
Transgenesis
Proefschrift ter verkrijging van
de graad Doctor aan de Universiteit Leiden,
op gezag van de Rector Magnificus Prof. Mr. P.F. van der Heijden, volgens besluit van het College voor Promoties,
te verdedigen op donderdag 21 oktober 2010 klokke 15.00 uur
door
Geert J. Schenk Geboren te Hillegom
in 1979
Geert J. Schenk
Understanding Doublecortin-Like Kinase Gene Function Through Transgenesis Thesis, Leiden University
Date: September 2010 ISBN: 978-90-8891-195-8 Cover: © Antonio Hoogervorst
Published by: Uitgeverij BOXPress, Oisterwijk
Printed by: Proefschriftmaken.nl || Printyourthesis.com
© Geert J. Schenk
No parts of this thesis may be reproduced or transmitted in any form or by any means without written permission of the author.
Understanding Doublecortin-Like Kinase Gene Function Through
Transgenesis
Proefschrift ter verkrijging van
de graad Doctor aan de Universiteit Leiden,
op gezag van de Rector Magnificus Prof. Mr. P.F. van der Heijden, volgens besluit van het College voor Promoties,
te verdedigen op donderdag 21 oktober 2010 klokke 15.00 uur
door
Geert J. Schenk Geboren te Hillegom
in 1979
4
Promotiecommissie
Promotor Prof. Dr. E.R. de Kloet Co-promotor Dr. E. Vreugdenhil
Overige leden Dr. W.J. Scheenen (Radboud Universiteit Nijmegen) Dr. C.P. Fitzsimons (Universiteit van Amsterdam) Dr. A.G. de Boer
Prof. Dr. M. Danhof Prof. Dr. J. den Dunnen Prof. Dr. J. Noordermeer
The studies described in the thesis have been performed at the Division of Medical Pharmacology, Leiden/Amsterdam Center for Drug Research (LACDR) and Leiden University Medical Center (LUMC), Leiden, The Netherlands.
5
Voor Rob en Anneke
Promotiecommissie
Promotor Prof. Dr. E.R. de Kloet Co-promotor Dr. E. Vreugdenhil
Overige leden Dr. W.J. Scheenen (Radboud Universiteit Nijmegen) Dr. C.P. Fitzsimons (Universiteit van Amsterdam) Dr. A.G. de Boer
Prof. Dr. M. Danhof Prof. Dr. J. den Dunnen Prof. Dr. J. Noordermeer
The studies described in the thesis have been performed at the Division of Medical Pharmacology, Leiden/Amsterdam Center for Drug Research (LACDR) and Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Voor Rob en Anneke
6
Table of Contents
Abbreviations Chapter 1 General Introduction
Chapter 2 A potential role for calcium / calmodulin-dependent protein kinase- related peptide in neuronal apoptosis: in vivo and in vitro evidence.
Chapter 3 Over-expression of the DCLK gene transcript CARP decreases CA3/CA1 network excitability
Chapter 4 Hippocampal CARP over-expression solidifies consolidation of contextual fear memories
Chapter 5 Over-expression of δC-DCLK-short in mouse hippocampus results in a more anxious behavioural phenotype
Chapter 6 General discussion Chapter 7 Summary
Nederlandse samenvatting Curriculum Vitae
List of publications
7
Abbreviations
ACSF Artificial Cerebrospinal Fluid ADX Adrenalectomy
BDNF Brain Derived Neurotrophic Factor CA Cornu Ammonis
Calb2 Calbindin2; Calretinin
CaMK Calcium/Calmodulin dependent protein kinase
CARP Calcium/Calmodulin dependent protein kinase Related Peptide CS: Conditioned Stimulus
D1 Dopamine 1 DCL Doublecortin-Like DCLK Doublecortin-Like Kinase DCX Doublecortin
DEPC Diethylpyrocarbonate DG Dentate Gyrus
Epac2 Exchange protein activated by cyclic AMP 2 EPM: Elevated Plus Maze
ERK Extracellular signal-Regulated Kinase fEPSP field Excitatory Post Synaptic Potential GABA g-Amino-Butyric Acid
GAPDH Glyceraldehyde 3-phosphate Dehydrogenase GDP Guanosine Diphosphate
Gef Guanine exchange factor
Grb2 Growthfactor receptor-bound protein 2 GTP Guanine Tri Phosphate
HPA Hypotalamic Pituitary Adrenal LT-82 Clostridium Sordelli Lethal Toxin-82 LTD Long Term Depression
LTP Long Term Potentiation
MAPK Mitogen-Activated Protein Kinase NMDA N-Methyl-D-Aspartic acid NT-3 Neurotrophin-3
PP Paired Pulse
PSA Population Spike Amplitude ROD Relative Optical Density SH Src Homology domain Sos Son of Sevenless SP Serine/Proline TG Transgenic
Trk Tropomyosin Receptor Kinase UCS Unconditioned Stimulus WT Wild-Type
Table of Contents
Abbreviations Chapter 1 General Introduction
Chapter 2 A potential role for calcium / calmodulin-dependent protein kinase- related peptide in neuronal apoptosis: in vivo and in vitro evidence.
Chapter 3 Over-expression of the DCLK gene transcript CARP decreases CA3/CA1 network excitability
Chapter 4 Hippocampal CARP over-expression solidifies consolidation of contextual fear memories
Chapter 5 Over-expression of δC-DCLK-short in mouse hippocampus results in a more anxious behavioural phenotype
Chapter 6 General discussion Chapter 7 Summary
Nederlandse samenvatting Curriculum Vitae
List of publications
Abbreviations
ACSF Artificial Cerebrospinal Fluid ADX Adrenalectomy
BDNF Brain Derived Neurotrophic Factor CA Cornu Ammonis
Calb2 Calbindin2; Calretinin
CaMK Calcium/Calmodulin dependent protein kinase
CARP Calcium/Calmodulin dependent protein kinase Related Peptide CS: Conditioned Stimulus
D1 Dopamine 1 DCL Doublecortin-Like DCLK Doublecortin-Like Kinase DCX Doublecortin
DEPC Diethylpyrocarbonate DG Dentate Gyrus
Epac2 Exchange protein activated by cyclic AMP 2 EPM: Elevated Plus Maze
ERK Extracellular signal-Regulated Kinase fEPSP field Excitatory Post Synaptic Potential GABA g-Amino-Butyric Acid
GAPDH Glyceraldehyde 3-phosphate Dehydrogenase GDP Guanosine Diphosphate
Gef Guanine exchange factor
Grb2 Growthfactor receptor-bound protein 2 GTP Guanine Tri Phosphate
HPA Hypotalamic Pituitary Adrenal LT-82 Clostridium Sordelli Lethal Toxin-82 LTD Long Term Depression
LTP Long Term Potentiation
MAPK Mitogen-Activated Protein Kinase NMDA N-Methyl-D-Aspartic acid NT-3 Neurotrophin-3
PP Paired Pulse
PSA Population Spike Amplitude ROD Relative Optical Density SH Src Homology domain Sos Son of Sevenless SP Serine/Proline TG Transgenic
Trk Tropomyosin Receptor Kinase UCS Unconditioned Stimulus WT Wild-Type
