Doublecortin-like kinase and neuronal differentiation
Dijkmans, T.F.
Citation
Dijkmans, T. F. (2009, October 14). Doublecortin-like kinase and neuronal differentiation. Retrieved from https://hdl.handle.net/1887/14055
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Appendix
COLORFIGURE FROMCHAPTE
E8,9AND10
ER4
Figure 8A. DCLK-short colocaliz
168 Appendix
0 min
NGF DCLK F-actin M
5 min
60 min
24 hrs
Figure 8. DCLK-short colocalizes with filamg period of NGF treatment, total DCLK-shor (B; in green) and filamentous actin (in re confocal microscopy. As indicated by colocalize at actin-rich structures, partic treatment appears to increase DCLK-s pSer30-DCLK suggest stronger colocaliz microns and white-lined boxes define adja DCLK-short.
zes with F-actin in growth cones
Merge Zoom
mentous actin in growth cones. For differentg rt protein (A; in green), pSer30-DCLK-short d, rhodamine-phalloidin) was visualized by arrow heads, DCLK-short and F-actin cularly at growth cones. Durance of NGF short enrichment at actin structures and zation with actin. Scale bars indicate 10
cent magnifications. DCLK abbreviates
Figure 8B. pSer30 DCLK-short colo
0 min
NGF pSer30DCLK F-actin
5 min
60 min
24 hrs
Figure 8. DCLK-short colocalizes with filamg period of NGF treatment, total DCLK-shor (B; in green) and filamentous actin (in re confocal microscopy. As indicated by colocalize at actin-rich structures, partic treatment appears to increase DCLK-s pSer30-DCLK suggest stronger colocaliz microns and white-lined boxes define adja DCLK-short.
calizes with F-actin in growth cones
Merge Zoom
mentous actin in growth cones. For differentg rt protein (A; in green), pSer30-DCLK-short d, rhodamine-phalloidin) was visualized by arrow heads, DCLK-short and F-actin cularly at growth cones. Durance of NGF short enrichment at actin structures and zation with actin. Scale bars indicate 10
cent magnifications. DCLK abbreviates
Figure 9. Endogenous DCLK-s the actin cyt
A DCLK F-ac
170 Appendix
hours NGF
B pSer30DCLK F-a
24
p
ours NGF 24 h o
Figure 9. DCLK-short colocalizes with filamen the actin cytoskeleton was disrupted by 3 houy p y short protein (A; in green), pSer30-DCLK-sho rhodamine-phalloidin) was visualized by confo actin cytoskeleton intact, whereas cytochalasin (No CytoD versus CytoD). As indicated by arr at actin-rich structures, particularly at gro colocalizing structures similar to actin aggreg indicates DCLK-short-F-actin interaction. DCLK 10 microns.
short localization depends on toskeleton
ctin Merge
No CytoD
actin Merge
CytoD
g
No CytoD CytoD
ntous actin. After 24 hours of NGF treatment, urs of Cytochalasin D treatment. Total DCLK-y ort (B; in green) and filamentous actin (in red;
cal microscopy. Control treatment retained the n D produced actin aggregates after disruption row heads, DCLK-short and F-actin colocalize wth cones in control treatments, whereas gates were formed by the DCLK signal and K abbreviates DCLK-short. Scale bars indicate
