University of Groningen Sec translocase in action Komarudin, Amalina Ghaisani
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(2) Appendices Acknowledgement About the Author List of Publications.
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(4) Appendices. ACKNOWLEDGMENTS Alhamdulillahirobbil ‘alamin.1SBJTFUPUIF"MNJHIUZ "MMBI485XIPIBTHSBOUFENFBO PQQPSUVOJUZBOEDPVSBHFUPmOJTINZ1I%*OEFFE UIJTKPVSOFZJTOPUBTTUSBJHIUBTB IJHIXBZ UIFSFXFSFNBOZUVSOTBOEIBSETIJQT*IBWFSFDFJWFEBMPUPGTVQQPSU HVJEBODF BOELJOEOFTTGSPNQFPQMFXIPN*NFUUISPVHIPVUUIJTKPVSOFZ5IFSFGPSF *XPVMEMJLF UPUIBOLBMMPGZPV 'JSTUPGBMM *XPVMEMJLFUPFYQSFTTNZHSBUJUVEFUPNZQSPNPUPS Prof. Arnold Driessen. %FBS"SOPME *EPOUIBWFXPSETUPUIBOLZPVGPSUIFPQQPSUVOJUZUPEPUIF1I%JOUIF .PMFDVMBS.JDSPCJPMPHZHSPVQ*IJHIMZBQQSFDJBUFZPVSHVJEBODF DPOTUBOUTVQQPSU UJNF QBUJFODFBOEFODPVSBHFNFOUUISPVHIPVUNZ1I% FTQFDJBMMZEVSJOHUIFIBSEUJNFT 8JUIPVUZPVSIFMQ UIJTUIFTJTXJMMOPUDPNFJOSFBMJUZ5IBOLZPVWFSZNVDIGPSFWFSZUIJOH *XPVMEMJLFUPUIBOLUPNZEBJMZTVQFSWJTPSEVSJOHNZmSTUZFBSPG1I% Dr. Ilja Kusters. %FBS*MKB *TUJMMSFNFNCFSIPXTIZ*XBTPODF*TUBSUFENZ1I%5IBOLZPVWFSZNVDI GPSUIFUSBJOJOHBOEZPVSRVJDLSFTQPOTFTUPNZRVFTUJPOFWFOUIPVHIZPVBSFBMSFBEZ JO#PTUPO*IJHIMZBQQSFDJBUFJU*XJTIZPVBMMUIFCFTUGPSZPVSMJGF4QFDJBMUIBOLTUP the reading committee members, Prof. Jan Marten van Dijl, Prof. Alexej Kedrov and Prof. Giovanni Maglia5IBOLZPVTPNVDIGPSZPVSQSFDJPVTUJNFUPFWBMVBUFUIJTUIFTJT To the paranymphs, Marten and Max, dank jullie wel! Marten, you helped me a lot (I really mean it, A LOT 5IBOLZPVTPNVDIGPSBMXBZTCFJOHUIFSF FTQFDJBMMZXIFO*BN in doubt or when I need second opinion about my results, you successfully altered an iFMFQIBOUwUPNFJOUPBiNPVTFw*EJEFOKPZPVSUJNFXIFOXFXFSFQBSBOZNQICVEEJFT GPSUJNFTþ Okidoki, bedankt en tot ziens! Max *LOPXZPVTJODFZPVXFSFTUVEFOUPG &MLF5IBOLZPVGPSIFMQJOHNFBMPUEVSJOHNZMBTUZFBSJOUIFMBC"OEBMTPUIBOLZPV GPSUSBOTMBUJOHUIF%VUDITVNNBSZ*XJTIZPVUXPBMMUIFCFTUþ *XPVMEMJLFUPFYQSFTTNZHSBUJUVEFUPDr. Dessy Natalia, as my supervisor during my CBDIFMPSBOENBTUFSTUVEZJO*OTUJUVU5FLOPMPHJ#BOEVOH *5# 5IBOLZPVGPSFODPVSBHJOH NFBOEJOTQJSJOHNFUPEPB1I%5PBMMUIFDPNNJUUFFPGITB-Zernike Institute for Advanced Materials Sandwich program UIBOLZPVGPSUIFJOUFSOTIJQPQQPSUVOJUZBT BQQBSFOUMZJUMFENFUPB1I%QSPHSBNJO.PMNJDHSPVQJan-Pieter UIBOLZPVGPSBMMUIF UFDIOJDBMTLJMMTZPVUBVHIUNFEVSJOHNZNBTUFSSFTFBSDI*IBWFMFBSOFEBMPUGSPNZPV Mickel Hansen and Prof. Ben Feringa JUXBTBOIPOPSGPSNFUPDPMMBCPSBUFXJUIZPV 5IBOLZPVGPSUIFGSVJUGVMEJTDVTTJPOProf. Dirk-Jan Scheffers and Dr. Juke Lolkema,. 117.
(5) UIBOLZPVTPNVDIGPSBMMUIFBEWJDFBOETVHHFTUJPOTEVSJOHUIFXPSLEJTDVTTJPO*SFBMMZ BQQSFDJBUFJU My dear Antonella UIBOLZPVGPSCFJOHBSFBMMZHPPEGSJFOEBOEBMXBZTMJTUFOJOHUPNF *TUJMMSFNFNCFSUIFUJNFXFIBEXIFOXFXFSFNBTUFSTUVEFOUTBU.-**MBC5IBOLZPV GPSBMMZPVSIFMQBOEGPSBMXBZTCFJOHUIFSFEVSJOHNZVQTBOEEPXOT*XJTIZPVBMMUIF CFTUJOZPVSGVUVSF%FBSDanae, we have a lot in common, even almost the same date PGCJSUI *FOKPZFEBMMUIFHPPEUJNFXFIBE:PVTVQQPSUFENFEVSJOHNZAMJCSBSZUJNF BOEUIFSFGPSFZPVCFDPNFNZMJCSBSZNBUF*XJMMOFWFSGPSHFUPVSDIJUDIBUXIFOXF mMMFEUIFMJRVJEOJUSPHFOUBOLBOE*SFBMMZBQQSFDJBUFZPVSHFOFSPVTTVQQPSUTFWFSZTJOHMF UJNF*OFFEUPTQJOEPXONZCJHTPVQZDVMUVSFT5IBOLZPVMay the Force be with you. To the member of the Sec group: Sabrina, Yanping, Anne-Bart, Irfan and Intan UIBOL ZPVTPNVDIGPSBMMZPVSIFMQ UFDIOJDBMUBMLTBOEWBMVBCMFBEWJDFUPNZFYQFSJNFOUT *XPVMEMJLFUPUIBOLUIFTVQFSNPNTJO.PMNJDHSPVQ%FBSElke, my fairy godsister :) I FOKPZFEBMPUPVSDIBU OPUPOMZBCPVUSFTFBSDICVUBMTPNPUIFSIPPEMJGF*TUJMMSFNFNCFS you taught me how to sing in de maaneschijn in the lab (~van de dikke dikke dikke UBOUF,Fþ :PVBSFBMXBZTGPSNFBOE*EPOUOFFEUIFHMBTTFT Ewa UIBOLZPV GPSUIFBEWJDF FTQFDJBMMZEVSJOHNZmSTUZFBSPG1I%BOEIPXUPHFUUISPVHIJUXJUIUIF QSFHOBODZMin JUJTTPOJDFUPLOPXUIBU*BNOPUBMPOFEPJOHUIJT "MMPGZPVBSFUSVMZ BTVQFSNPNBOETVDIBOJOTQJSBUJPOGPSNF Janny and Greetje UIBOL ZPV TP NVDI GPS BMXBZT UBLJOH DBSF PG FWFSZUIJOH JO UIF MBCBOEBMXBZTXJMMJOHUPIFMQNFGreetje, coincidently you were always there when I IBEQSPCMFNXJUIUIFIFJHIUT JTUIFSBDLUPPUBMMPSBN*UPPTIPSU .BZCFUIFMBUUFSJT SJHIUIBIB Jeroen and Susan UIBOLZPVTPNVDIGPSPSHBOJ[JOHUIFFO[ZNFTBOE TFRVFODJOH "MTPB#*(UIBOLZPVUPBMMUIFNFNCFSTPG.PMNJD%FBS Aleksandra, Dzien dobry! 5IBOLZPVGPSBMMUIFUJNFTXFXBEJOUIFMBCBOEJOUIFEBSLSPPN 'PSTVSF*XJMMNJTT the cat astronaut and your singing A,VQVMJOBLVQVMJOB PILVQVMJOBw%[J˒LVK˒þ :) Hyan Yong *SFBMMZFOKPZFEPVSHPPENPSOJOHTXJUIannyeonghaseyo-selamat pagi! It feels so HPPEUPTBZTPNF,PSFBOXPSETUPBOBUJWF,PSFBOGamsahamnida! Niels,UIBOLZPV very much for your help especially when I was struggling with the spectrophotometer BOE'1-$JOUIFDPMESPPNRiccardo, you are always willing to help me to set up the DFMMEJTSVQUPSBOE*BNTPHSBUFGVMUIBUZPVSPGmDFIBTBWFSZHPPETUPDLPGTXFFUT Grazie! Samta and Jeanine UIBOLZPVGPSUIFQSBDUJDBMTLJMMTZPVUBVHIUNF8JUIPVU. 118.
(6) Appendices. ZPVSIFMQ *XJMMOPUNBTUFSUIFNAnnarita, Yvonne, Sasha, Ana, Fernando, Carsten, 5HWR)DELROD,XOLLD=VRÀ$QGUH(ULN0HQQR/LWR/RHV-DYLHU*RVLD;LDRDQ Nienke, Laszlo, Lucia, Ciprian, Parichita, Alka, Ahsen, Laura and for all of you that *DPVMEOPUNFOUJPOUIBOLZPVGPSBMMUIFHPPEUJNFTBOEHSFBUNFNPSJFTJO.PMNJD* XJTIZPVBMMUIFCFTU My sincere gratitude to the incredible secretaries Bea, Anmara and Jan Bea and Anmara UIBOLZPVWFSZNVDIGPSBMMZPVSIFMQBOEFODPVSBHJOHXPSETEVSJOHNZ1I% FTQFDJBMMZXIFO*SFDFJWFEiUIFMFUUFSwGSPNUIFUBYPGmDF*IBEOPJEFBXIBUUPEPCVU ZPVUXPIFMQFENF"-05BOEXFSFBMXBZTCFJOHUIFSF*XJMMOFWFSGPSHFUZPVSLJOEOFTT Marco van der Vinne and Jos van Griesven UIBOLZPVWFSZNVDIGPSZPVSUSFNFOEPVT help to get through the long adventure'JOBMMZ JUFOETXJUIBHPPEPVUDPNFBOEJOEFFE JUJTBIVHFSFMJFG Rachael Yates and Julia Termeer NZUFBDIFSTBUUIFmSTUTUBHFPGNZNVTJDBMKPVSOFZ 'PSTVSF*XJMMDPOUJOVFUIJTKPVSOFZJO*OEPOFTJB 5IBOLZPVGPSTVDIBOJOTQJSBUJPOUP NFUPCFDPNFBWJPMJOJTUJannie, Janine, Gerjanne, Irene, and all the staff of KITS ,JOEFSEBHWFSCMJKG UIBOLZPVGPSUBLJOHDBSFPG%JOBSBXIFO*XBTJOUIFMBC Dina UIBOL ZPV GPS BMXBZT IFMQJOH NF XJUI UIF JMMVTUSBUJPO BOE NBLJOH UIF DPWFS PG UIJTUIFTJT*SFBMMZFOKPZFEPVSDIJUDIBUJOSundaneseMBOHVBHFSemoga Alloh selalu memudahkan urusan Dina ya aamiin.. Living far away from home feels easier with all the friendship from the big Indonesian community, the Kimia ITB family, the Groningen Indonesian Moslem Community (de Gromiest) and the Indonesian Student Association in Groningen (PPI Groningen): AliLiany, Reren, Teh Susan-Mas Bino, Teh Alia-Kak Izul, Teh Faizah-Mas Pandji, Kak Irfan-Teh Liza Miaw, Teh Astri, Teh Eryth-Kak Iqbal,Teh Lia-Kak Robby, Yosay-Ali, Kak Fean, Masyitha, Intan-Mas Rully, Mbak Nuri-Mas Adhyat, Ucon-Meisha, Ibu Rini-Pak Asmoro, Mbak Irma-Mas Ega, Teh Monik-Mas Fajar, Mbak Ayu-Mas Zainal, 0EDN$ÀIDK0DV5LVWLRQR0EDN$QGLV0DV$NEDU%X5RKPDK3DN7DWDQJ0EDN Fitri-Mas Kuswanto, Mbak Icha-Mas Krisna, Mbak Putri-Mas Amak, Mbak UciMas Joko, Mbak Cici-Mas Riswandy, Anis-Didin, Mbak Septi-Mas Latief, Mbak Mawa-Mas Habibie, Teh Dita-Mas Ivan, Mbak Inna-Mas Agung, Mbak Retno-Mas Khairul, Yassaroh-Mas Surya, Mbak Rosel-Mas Didik, Mbak Ira, Sofa-Yudi, Teh Anya-Mas Dimas, Mbak Jean-Mas Chalis, Mbak Nur, Mbak Nadia-Mas Zakki, Cika0DV$GH6DOYD0EDN7LQD3DN7DXÀN0EDN$UXP0DV/DQD0DV$]ND0DV Adjie, Mas Pandji, Teh Inda, Mbak Frita, Rieza, Ibu Ima, Mbak Laksmi-Mas Kadek,. 119.
(7) Mbak Wiwiek-Mas Donny, Teh Ghina-Mas Azzam, Mbak Atika, Mbak Rani, Mbak Ria, mbak Diani Ibu Elvira, Ibu Roos, Mbak Enny, Uwak Delfzijl, Bude Arie-Om Herman, Bude Nunung dan semua yang tidak bisa saya sebutkan satu per satu. Terimakasih atas bantuan, kehangatan dan kekeluargaannya selama ini. Semoga Alloh senantiasa memberikan balasan yang berlipat ganda atas kebaikannya kepada kami. To all the children and the teachers of Indonesian Moslem Kids Community (de Gromiest Kinderen or Pengajian Anak) UIBOLZPVGPSUIFFOUIVTJBTNBOEQPTJUJWF FOFSHZFWFSZ4VOEBZ*OEFFE*XJMMNJTTUIFBUNPTQIFSFSemoga semakin berkah dan anak-anak semakin sholeh dan sholehah. Aamiin.. Tepuk satu! :) For Planetenlaan 171 family: 'XKLWD 1LNHQ <RYL 'HGHV 7HK 6RÀ 1RYLND 'DVKD 5LVD 1LVD 9DQLD Erin, and Asa5IBOLZPVGPSBMMUIFHPPEUJNFTXFIBEMohon maaf kalau ada salah dari kami ya :) To my beloved parents, Mamah dan BapaMah.. Pa.. akhirnya sekolah Adik selesai Nuhun ya Mah Pa atas kepercayaan dan doa yang tidak pernah putusnya buat Adik, Aa dan neng Naya"MTPUPNZQBSFOUTJOMBX Mamah Bapa Leuwi Panjang, terimakasih atas doa-doanya dan semangatnya. To my other half, Amalia*BNPOMZNJOVUFTZPVOHFSUIBOZPVCVUZPVBSFUSVMZNZ JOTQJSBUJPOTJODF*XBTBMJUUMFHJSM5IBOLZPVGPSZPVSMPWF XPSETPGFODPVSBHFNFOUBOE QBUJFOUMZBMXBZTMJTUFOJOHUPNF*XJTIZPVBMMUIFIBQQJOFTTUPHFUIFSXJUIMas Inoel and Aiko"OEUPNZZPVOHFSTJTUFS Kiki *BMXBZTBENJSFZPVSQFSTJTUFODF:PVXJMMCFBHSFBU BSDIJUFDU *LOPXJU5IBOLZPVGPSZPVSMPWFBOETVQQPSUUntuk seluruh keluarga besar (Alm) Eman Sulaeman dan (Alm) Toyib Sastraatmaja, terimakasih atas doa-doa dan dukungannya. Insya Alloh sebentar lagi Adik pulang dan bisa kumpul sama-sama lagi. Finally, my deepest gratitude to the light of my life, my dearest Dinara and my beloved husband AzisDinara ZPVBSFUIFQJMMBSPGNZTUSFOHUIBOEUIFSFBTPOXIZ*LFFQHPJOH *TUJMMSFNFNCFSIPXZPVPGGFSFENFZPVSQFOBOETBJEiUIJTGPSZPVNPN UPTUVEZw :PVSTVQQPSU TNJMFTBOEIVHNFBOBXPSMEUPNF Azis XFTUBSUFEUIJT1I%KPVSOFZ UPHFUIFS:PVBMXBZTCFMJFWFJONFBOEFODPVSBHFNFJOBMMPGUIFFGGPSUT8JUIPVUZPVS VODPOEJUJPOBMMPWFBOEVOMJNJUFETVQQPSU *XPVMEOPUIBWFDPNFUIJTGBS5IBOLZPVGPS IBWJOHNFJOZPVSMJGF Ibu sayang sama Ayah. &WFSZ FOE JT B OFX CFHJOOJOH *U JT UJNF UP CFHJO B OFX BEWFOUVSF JO NZ MJGF Alhamdulillahirabbilalamin.... 120.
(8) Appendices. ABOUT THE AUTHOR Amalina Ghaisani Komarudin was born in Bandung, *OEPOFTJB PO'FCSVBSZUI*O+VMZ TIF HSBEVBUFE XJUI #4D EFHSFF JO $IFNJTUSZ )POPST QSPHSBN GSPN*OTUJUVU5FLOPMPHJ#BOEVOH *5# 4IF did her research at the Biochemistry laboratory under UIF TVQFSWJTJPO PG %S %FTTZ /BUBMJB %VSJOH IFS CBDIFMPS TUVEZ TIF EJE BO JOUFSOTIJQ JO 15 ,VKBOH Sud-Chemie, Karawang, Indonesia in the Department PG3FTFBSDIBOE%FWFMPQNFOUXIFSFTIFXBTJOWPMWFE JOQSPKFDUPGDBUBMZTUTDIBSBDUFSJ[BUJPO 4IFDPOUJOVFEIFSFEVDBUJPOCZFOSPMMJOHB'BTU5SBDL.BTUFSQSPHSBNJO*5#JO NBKPSJOHBU#JPDIFNJTUSZ0OIFSMBTUTFNFTUFS TIFEJEBOJOUFSOTIJQGPSIFSNBTUFS UIFTJTJO1SPG"SOPME%SJFTTFOTHSPVQ .PMFDVMBS.JDSPCJPMPHZ%FQBSUNFOU 6OJWFSTJUZ PG (SPOJOHFO UIF /FUIFSMBOET 4IF JT JOWPMWFE JO .VMUJESVH 5SBOTQPSUFS -NS$% PG Lactococcus lactisQSPKFDUBOEDPNQMFUFEIFSNBTUFSEFHSFFJOXJUIcum laude QSFEJDBUF4IFQVSTVFEIFS1I%JOUIFTBNFSFTFBSDIHSPVQJOBOEXPSLFEPO4FD translocase in Escherichia coli %VSJOHIFSTQBSFUJNF TIFFOKPZTCBLJOHTPNFDBLFTBOEQMBZJOHWJPMJO#FTJEFT TIF MJLFTUPEPBWPMVOUBSZXPSL$VSSFOUMZ TIFUBLFTQBSUJO*OEPOFTJBO.PTMFN,JET4PDJFUZ in Groningen (de Gromiest Kinderen), in particular teaching the toddler group every 4VOEBZ. LIST OF PUBLICATIONS •. Adharis A, Ketelaar T, Komarudin AG -PPT, 4ZOUIFTJTBOE4FMG"TTFNCMZ PG%PVCMF)ZESPQIJMJDBOE"NQIJQIJMJD#MPDL(MZDPQPMZNFST#JPNBDSPNPMFDVMFT. •. van der Berg JP, Madoori PK, Komarudin AG, Thunnissen A-M, Driessen AJM #JOEJOHPGUIF-BDUPDPDDBM%SVH%FQFOEFOU5SBOTDSJQUJPOBM3FHVMBUPS-NS3 UP*UT-JHBOETBOE3FTQPOTJWF1SPNPUFS3FHJPOT1-P40/& F. •. Komarudin AG %SJFTTFO "+. 4FD"NFEJBUFE 1SPUFJO 5SBOTMPDBUJPO 5ISPVHI4FD:&($IBOOFM TVCNJUUFE. •. Komarudin AG )BOTFO.+ 'FSJOHB#- %SJFTTFO"+. 4FD:&(.FEJBUFT 5SBOTMPDBUJPOPG4FDSFUPSZ1SPUFJO.PEJmFEXJUI/PO1PMZQFQUJEF4FHNFOUT UPCF submitted). 121.
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(11) ISBN ISBN(printed): (printed):978-94-034-1773-8 978-94-034-1773-8 ISBN ISBN(electronic): (electronic):978-94-034-1772-1 978-94-034-1772-1.
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