University of Groningen Sec translocase in action Komarudin, Amalina Ghaisani

University of Groningen

Sec translocase in action

Komarudin, Amalina Ghaisani

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Publication date:

2019

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Komarudin, A. G. (2019). Sec translocase in action: Translocation initiation and processivity. University of

Groningen.

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Propositions

belonging to the PhD thesis

Sec translocase in action

Translocation initiation and processivity

Amalina Ghaisani Komarudin

1. The two-helix finger (2HF) domain of SecA plays an important role in SecA mediated protein translocation. (Chapter 1)

2. A SecA mutant with a symmetrically shorted 2HF is still functional. (Chapter 2) 3. SecYEG is permissive for the translocation of proOmpA substrates that are

chemically modified, except when the modifications occur at the extreme N-terminus. (Chapter 3)

4. Proper alignment of the first 20 amino acids of the mature N-terminus of proOmpA is critical for channel opening and translocation. (Chapter 3)

5. The conserved and essential Tyrosine 794 residue of SecA can be replaced by a cysteine modified with a particularly sized fluorophore. (Chapter 4)

6. Updating a lab journal regularly is a good way to appreciate what you have done and to complete the project. Record everything and keep going.

7. Doing a PhD and raising a daughter simultaneously is challenging yet feasible. 8. “For indeed, with hardship [will be] ease. Indeed, with hardship [will be] ease.

(Al-Quran, Chapter 94, verse 5-6)”

9. An experimental protocol and a cake recipe have one thing in common: sometimes optimization is needed for a better result.

10. Dealing with the unfair Dutch tax office decision is one of the many ways to keep awake at night.