0 1 2 3 4 5
empty vector Sc[C.t.cbh1] Sc[H.g.cbh1] Sc[T.e.cbh1] Sc[Ac.t.cbh1] Sc[N.f.cbh1] Sc[T.r.cbh1]
% Avicel hydrolysis
A
B
C
D
0 10 20 30 40 50 60
empty vector
microM MU released per min
0 1 2 3 4 5 6 7 8 9 10
empty vector Sc[H.g.cbh1] Sc[T.a.cbh1] Sc[T.e.cbh1] Sc[N.f.cbh1] Sc[P.j.cbh1] Sc[G.z.cbh1] Sc[N.h.cbh1] Sc[F.p.cbh1] Sc[As.t.cbh1] Sc[P.c.cbh1] Sc[N.c.cbh1] Sc[C.t.cbh1] Sc[Ac.t.cbh1] Sc[T.r.cbh1]
% Avicel Hydrolysis
24 Hours 48 Hours 0.1
1 10 100 1000
uM MU released per min
Additional file 1. Secreted CBH1 activity. Production of CBH1 enzymes in YPD (A; B) and in SCD-URA medium (pH 6) (C; D) by recombinant strains expressing cbh1 genes as indicated. Culture
supernatant samples were taken after 3 days cultivation for the enzyme assays. A.; C. Secreted MULac activity (microM MU released per minute) in the. B; D. Percentage Avicel hydrolysis by supernatants after 24 (grey bars) and 48 (black bars) hours incubation. The values shown are the mean values of three repeats ± standard deviation (SD).
