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Reinventing microinjection : new microfluidic methods for cell biology

Sonneville, J. de

Citation

Sonneville, J. de. (2011, November 16). Reinventing microinjection : new microfluidic methods for cell biology. Retrieved from

https://hdl.handle.net/1887/18086

Version: Corrected Publisher’s Version

License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden

Downloaded from: https://hdl.handle.net/1887/18086

Note: To cite this publication please use the final published version (if applicable).

(2)

Reinventing Microinjection

New microfluidic methods for cell biology

Proefschrift

ter verkrijging van de graad van Doctor aan de Universiteit Leiden,

op gezag van Rector Magnificus prof.mr. P.F. van der Heijden, volgens besluit van het College voor Promoties te verdedigen op woensdag 16 november 2011

klokke 16.15 uur door Jan de Sonneville geboren te Amsterdam

in 1980.

(3)

Reinventing Microinjection

New microfluidic methods for cell biology

Proefschrift

ter verkrijging van de graad van Doctor aan de Universiteit Leiden,

op gezag van Rector Magnificus prof.mr. P.F. van der Heijden, volgens besluit van het College voor Promoties te verdedigen op woensdag 16 november 2011

klokke 16.15 uur door Jan de Sonneville, geboren te Amsterdam

in 1980.

(4)

reinventing microinjection

Chapter 1 Chapter 2

Chapter 3

Chapter 4

Chapter 5

Chapter 6 Summary Samenvatting

Acknowledgements Curriculum Vitae Publications

Introduction

A versatile microfluidic flow cell for studying the dynamics of shear-stress in- duced actin reorganization in renal cells

Determination of the size distribution of blood microparticles directly in plas- ma using atomic force microscopy and microfluidics

A High-Throughput Screen for Tuber- culosis Progression

Automated microinjection of cell-poly- mer suspensions in 3D ECM scaffolds for high throughput quantitative cancer invasion screens

Appeal for better data management

1 19

39

59

77

97 115 121 127 131 133

Promotiecommissie:

Promotores

Prof. dr. J.P.Abrahams Prof. dr. M.H.M. Noteborn

Copromotor

dr. M.E. Kuil

Overige Leden

Prof.dr. H.P. Spaink Prof.dr. J. Brouwer Prof.dr. T.H. Oosterkamp Prof.dr. S. Osanto

dr. E.H.J. Danen

© by Jan de Sonneville. All rights reserved.

The publication of this thesis was financially supported by Märzhäuser, ZF-screens, Eppen- dorf and Life Science Methods.

A digital version of this thesis can be obtained from http://ub.leidenuniv.nl.

table of contents

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