Reinventing microinjection : new microfluidic methods for cell biology
Sonneville, J. de
Citation
Sonneville, J. de. (2011, November 16). Reinventing microinjection : new microfluidic methods for cell biology. Retrieved from https://hdl.handle.net/1887/18086
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden Downloaded from: https://hdl.handle.net/1887/18086
Note: To cite this publication please use the final published version (if applicable).
Stellingen
1. Shear stress applied to proximal tubular cells changes their cellu- lar phenotype, while preserving the average location. (This thesis)
2. The application of a detachable microfluidic channel does not change the qualitative shape of a platelet microparticle size dis- tribution. (This thesis)
3. The absence of lungs in zebrafish does not exclude studying the progression of Mycobacterium Tuberculosis infection by automated high-throughput microinjection. (This thesis)
4. Automated injection of a cell-polymer solution into collagen gel allows for in-vitro 3D tumor drug screens using primary tumor cells in one week. (This thesis)
5. In-vitro experiments aimed at mimicking in-vivo conditions require highthroughput experimentation to reach statistical significant conclusions. (This thesis)
6. A social network with the ability to store experimental data and protocols can improve knowledge sharing and reproducibility of scientific results. (This thesis)
7. Biological experimentation takes biological complexity more ef- fectively into account than physical modeling.
8. Physical and statistical reasoning can lead to simplification of the interpretation of biological experiments, by putting the biological complexity in a “Black Box” .
9. An engineer’s train of thought is not the best sequence for pre- senting an idea.
10. In research collaborations as well as in dancing, optimal results are obtained when people are open towards each other.
