RESEARCH
Redescription and molecular
characterisation of the fish ectoparasite Anilocra
capensis Leach, 1818 (Isopoda: Cymothoidae),
with description of six new species of Anilocra
Leach, 1818 from Africa
Rachel L. Welicky
1,2*and Nico J. Smit
1Abstract
Background: Anilocra capensis Leach, 1818 is the only named species of Anilocra Leach, 1818 from South Africa.
Anilocra is a large genus (> 40 species) with high levels of diversity reported from the Caribbean and Indo-West Pacific. Considering it is highly unlikely that all records of Anilocra from South Africa can be of a single species, the aim of this study was to better understand the diversity of Anilocra from this region and continent.
Methods: To redescribe A. capensis, the syntypes of A. capensis and specimens recorded as A. capensis from Africa
were borrowed from the Natural History Museum, London, UK, and The iZiko South African Museum, Cape Town. Newly collected fresh samples of A. capensis were collected from off Cape Town, South Africa. Morphological rede-scriptions of the syntypes, and other museum and fresh material were conducted. Fresh samples were used to charac-terise molecularly A. capensis using the mitochondrial cytochrome c oxidase subunit 1 gene (cox1).
Results: Morphological analyses demonstrated that apart from A. capensis there are six Anilocra species new to
sci-ence from Africa: Anilocra ianhudsoni n. sp., Anilocra bunkleywilliamsae n. sp., Anilocra paulsikkeli n. sp., Anilocra jovanasi n. sp., Anilocra angeladaviesae n. sp. and Anilocra hadfieldae n. sp. Of the species under study, specimens of A. capensis appear to demonstrate the most individual variation, which occurs in pleonite width, pleotelson form and uropod length. We determined that African species of Anilocra can be primarily differentiated by the proportional shape and size of the full body in dorsal view and pereonites 1, 6 and 7. Other defining morphological traits include proportional shape and size of the pereopods, and the antenna and antennula peduncles. Lastly, the molecular characterisation of A. capensis is provided and the interspecific divergence with Mediterranean species is smaller than that with Carib-bean species.
Conclusions: The results of this study provide a detailed redescription of A. capensis and the first molecular barcode
for this organism. Six new species of Anilocra from Africa are described, establishing that the diversity of Anilocra in this region is greater than previously known. With this new understanding of species differences, we can accurately conduct detailed molecular and ecological analyses of Anilocra from Africa with certainty of the organism under study.
Keywords: Africa, Anilocra, Cymothoid, Fish parasite, Molecular characterization, Morphological description
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Open Access
*Correspondence: rwelicky@gmail.com
2 Present Address: School of Aquatic and Fishery Sciences, University of Washington, 1122 NE Boat Street, Seattle, WA 98105, USA Full list of author information is available at the end of the article
Background
Members of the isopod fish parasitic family Cymothoidae Leach, 1818 conspicuously infest the skin, buccal cavity, or gill chamber of their hosts. This family has a global dis-tribution and there are more than 43 recognised genera [1]. One of the most commonly observed genera is Ani-locra Leach, 1818, and it remains one of the best studied ecologically [2–4]. In fact, Anilocra was the first genus of a marine fish parasite to be described from South Africa [5].
Leach [6] erected Anilocra to accommodate three spe-cies. One of these species was Anilocra capensis Leach, 1818, collected in the Cape region of South Africa. In Schioedte & Meinert’s (1879) redescription of A. capen-sis, the authors provided only two dorsal view drawings of the female, and one dorsal view drawing of the male [7]. Schioedte & Meinert (1879) did not examine Leach’s type-material. Since 1879, no further supplemental rede-scription of A. capensis has been published. As a result, nearly all reports of Anilocra infestation on South Afri-can marine fishes have been recorded as A. capensis.
The majority of Anilocra have been reported from tropical environments, e.g. [8–10]. Although cymothoids are generally more speciose in tropical than in temper-ate environments, tempertemper-ate systems are nonetheless diverse. Accordingly, Anilocra diversity in South Africa should be far greater than the single recorded species. This should particularly be the case as South Africa’s coastline encompasses two oceans that span temperate to subtropical ecosystems. Moreover, these ecosystems are suitable for a diversity of potential fish hosts.
To test the aforementioned prediction, a review of A. capensis based on the syntypes and all other available material was warranted. We seek to provide descriptions of species of Anilocra from South Africa and the African continent in order to clarify and describe the diversity of Anilocra from the eastern Atlantic and western Indian
Oceans. Moreover, we aim to provide molecular data for A. capensis so that future taxonomic studies can at a min-imum describe Anilocra from Africa as A. capensis or a different species of Anilocra.
Methods
Specimen collection
In March 2017, specimens identified as Anilocra capen-sis with locality data from Africa, along with the syntypes of A. capensis were borrowed from the Natural History Museum, London, UK (NHM). We examined other spec-imens of Anilocra from outside South Africa from NHM to confirm or reject if they were A. capensis. In July 2017, all specimens labelled as Anilocra from The iZiko South African Museum, Cape Town (SAM) were borrowed. In April 2018, live A. capensis were collected from the Hot-tentot seabream, Pachymetopon blochii Valenciennes, at two localities: Cape Town Harbour wall and the False Bay Yacht Club harbour. Infested fishes were speared on SCUBA under the direction and permitting of Two Oceans Aquarium. The fresh material was preserved in 80% ethanol for later morphological analysis (see Table 1
for sample sizes and locality data). For some specimens, leg tissue was extracted using forceps and placed in 96% ethanol for molecular analysis.
Molecular analysis
Genomic DNA was extracted from 8 of the freshly col-lected A. capensis. A rapid DNA extraction method as described in the KAPA Express Extract Kit (Kapa Biosys-tems, Cape Town, South Africa) was used. Polymerase chain reactions (PCR) were used to amplify a 710 bp frag-ment of the mitochondrial cytochrome c oxidase subunit gene (cox1) using the primer sets LCO 1490 and HCO 2198 [11]. PCR was performed using 12.5 μl Thermo Sci-entific DreamTaq PCR master mix (2×) (2× DreamTaq buffer, 0.4 mM of each dNTP, and 4 mM MgCl2), 1.25 μl
Table 1 Details of Anilocra species examined
a One specimen may be transitional
Collection locality Male Female
Anilocra capensis Off Cape Town (Harbor Wall), South Africa 8 9
False Bay, Cape Town, South Africa 26 38
Table Bay, Cape Town, South Africa 7
Cape Town region, South Africa 5
Anilocra ianhudsoni n. sp. Nahoon River, East London, South Africa 1 2
Anilocra bunkleywilliamsae n. sp. Mawalana Estuary, South Africa 1 2
Anilocra paulsikkeli n. sp. Delagoa Bay, Mozambique 2a 2
Anilocra jovanasi n. sp. Delagoa Bay, Mozambique 1
Anilocra angeladaviesae n. sp. Off Cape Blanc, Morocco 2 4
of each primer, 1 μl DNA, and 9 μl of PCR-grade nucle-ase free water (Thermo Fisher Scientific, Vilnius, Lithu-ania). Total volume per reaction was 25 μl, and PCR reactions were conducted using a ProFlex™ PCR thermal cycler (Applied Biosystems by Life Technologies). Reac-tions were amplified under the following PCR condiReac-tions: Stage 1, 94 °C for 5 min, Stage 2, 36 cycles of 94 °C for 30 s, 47 °C for 50 s, 72 °C for 2 min, and Stage 3, 72 °C for 10 min. PCR products were sent to a commercial sequenc-ing company (Inqaba Biotechnical Industries (Pty) Ltd, Pretoria, South Africa) for purification and sequencing in both directions. Obtained sequences were assembled, and chromatogram-based contigs were generated using Geneious Ver. 9.1. Sequences were aligned and trimmed to the length of the shortest sequence using MEGA 7 software program [12].
Using BLASTn (Basic Local Alignment Search Tool;
http://www.ncbi.nlm. nih.gov/blast), the obtained sequences were verified as belonging to the Isopoda. Mean intraspecific divergence for A. capensis and inter-specific divergence with all other Anilocra reported on GenBank were examined using p-distance and number of bp differences. We utilised the following sequences: Anilocra physodes Linneaus, 1758 (EF455817); Anilo-cra chromis Williams & Williams, 1981 (KY5622739); Anilocra brillae Welicky, Hadfield, Sikkel & Smit, 2017 (KY562744); Anilocra haemuli Williams & Williams, 1981 (KY562752); Anilocra prionuri Williams & Bunkley-Williams, 1986 (LC15954); and Anilocra clupei Williams & Bunkley-Williams, 1986 (LC160309). Newly-gener-ated sequences for Anilocra capensis were deposited in the GenBank database under the accession numbers MK450443-MK450452.
Morphological data
Specimens of Anilocra were processed according to the techniques described in [13, 14]. Dissection was permit-ted for specimens from SAM but not the NHM. Regard-ing the museum collection specimens in this study, SAM assigns one accession number per species and collection location, whereas NHM assigned one accession num-ber per individual in most cases. Accession numnum-bers from NHM are indicated with the abbreviation BMNH. Descriptions were prepared using DELTA (Descriptive Language for Taxonomy) [15] using a general character set for the Cymothoidae [16, 17]. Ratios and measure-ments were rounded off to one decimal place and were made using maximum values of the specific measured article. Measurements were taken and ratios determined from ovigerous and non-ovigerous female (♀), and male (♂) specimens. Pleotelson length (TL) and width (W) for all specimens examined are reported as TL × W. All measurements are reported in millimeters (mm). Higher
order classification follows Brandt & Poore [18]. Fish nomenclature follows FishBase [19].
To comply with the regulations set out in article 8.5 of the amended 2012 version of the International Code of Zoological Nomenclature (ICZN [20], details of all new taxa have been submitted to ZooBank. For each new taxon, the Life Science Identifier (LSID) is reported in the taxonomic summary.
Results
Specimen summary
We identified from the museum loans and field collection A. capensis and six new species of Anilocra. These speci-mens can be distinguished using the key provided here. Sample sizes and locality data for each species are given in Table 1.
Molecular analyses
Comparative sequence analysis indicated that the intraspecific divergence of A. capensis was 1–7 nt (0.04%). The interspecific divergence between A. capensis and A. physodes was 63–74 nt (15.5%). The interspecific divergence between A. capensis and Caribbean species of Anilocra were: A. chromis: 138–157 nt (24.9%), A. brillae: 138–157 nt (24.9%), A. haemuli: (KY562752) 136–154 (24.4%), A. prionuri: (LC159541) 136–157 (24.8%), and A. clupei: (LC160309) 132–144 (24.4%).
Taxonomy
Genus Anilocra Leach, 1818
Diagnosis. Diagnosis and synonymy in Bruce [21]. Body dorsally symmetric, weakly to strongly vaulted. Cephalon non-tri-lobed to weakly tri-lobed, anterior of frontal margin of rostrum blunt and folded down poste-riorly between antennule bases. Antennula shorter than antenna. Pereonite 5 or 6 widest, coxae 4–6 longer, more anteriorly pointed than 1–3. Pereopods 1–7 increase in size. Type-species: Anilocra cuvieri Leach, 1818.
Remarks
Leach [6] described three species: Anilocra cuvieri Leach, 1818, Anilocra mediterranea Leach, 1818 and Anilocra capensis. Anilocra cuvieri was designated as the type-spe-cies by Kussakin [22]. There are three Anilocra capensis syntypic specimens held at The Natural History Museum, London, presented by Leach from the Cape of Good Hope (see ‘Material examined’) [23].
Nearly half of the species of Anilocra accepted today were described in the 1980s, with many originating from the Indo-Pacific and Caribbean [8, 21]. Since 2001, just four new species have been described based on material
from the Caribbean [24], Chile [25], Lebanon [26] and the Gulf of Mexico [27]. In 2017, using morphological and molecular data, Anilocra brillae [24] from the Car-ibbean was identified and described based on specimens that were originally thought to be Anilocra haemuli [8]. This revision is an example of the cryptic nature of some Anilocra spp., and the importance of combining morpho-logical and molecular data whenever possible.
Of the skin-attaching cymothoids, species of Anilocra most closely resemble species of Renocila Miers, 1880 and Nerocila Leach, 1818, but generic level differences can be distinguished without dissection or stereoscopic microscope [28–30]. Briefly, in Anilocra spp. pereopod 7 is longer than 6, but in Renocila spp. pereopod 6 is similar in length to 7. The rostrum is not ventrally folded between the antennae in Renocila spp., but it is in Ani-locra spp. The cephalon of Nerocila spp. is posteriorly strongly trilobed, whereas the cephalon of Anilocra spp. is not tri-lobed to very weakly tri-lobed. Further compar-isons among cymothoid genera and generic features are detailed in Welicky et al. [24].
Anilocra capensis Leach, 1818
Type-host: Probable host Pachymetopon blochii referred
to as “Sargus Hottentotus” in Schiöedte & Meinert (1883) [7].
Type-material: BMNH 1979.329:207b (♀ 43.0 × 18.0)
(designated as the lectotype in the present study); BMNH 1979.329:207c (♀ 46.0 × 19.5); BMNH 1979.329:207a (♂ 35.0 × 10.0). Paralectotypes: BMNH 1979.329:207c (♀ 46.0 × 19.5), BMNH 1979.329:207a (♂ 35.0 × 10.0). Pre-sented by W. Leach in 1818 from Cape of Good Hope.
New material examined: From Cape Town Harbor Wall
(33°53′58″S, 18°25′35″E) SAMC-A091297: ♂ (22.0 × 7.0; 25.0 × 7.0; 26.0 × 8.5; 27.0 × 8.0; 27.0 × 8.5; 29.0 × 9.0; 31.5 × 9.0; 33.0 × 10.0), non-ovigerous ♀ (43.5 × 16.5; 44.5 × 20.0), ovigerous ♀ (43.0 × 18.5; 44.0 × 19.5; 45.0 × 19.5; 45.0 × 19.5; 49.0 × 20.0; 49.0 × 20.0), dam-aged ♀ (46.6 × 19.5). From False Bay Yacht Club Marina (34°11′32″S, 18°26′01″E) SAMC-A091298: ♂ (12.0 × 3.5; 15.0 × 4.0; 18.0 × 5.0; 18.5 × 5.0; 19.0 × 4.5; 19.0 × 5.0; 19.5 × 5.5; 20.0 × 5.0; 21.0 × 5.5; 21.0 × 5.5; 21.0 × 6.0; 22.0 × 6.0; 23.0 × 6.0; 23.0 × 6.5; 23.0 × 7.0; 23.0 × 8.0; 23.6 × 6.5; 24.0 × 5.0; 24.0 × 6.5; 24.0 × 7.0; 24.0 × 7.0; 25.0 × 7.0; 28.0 × 8.0; 28.0 × 8.0; 28.5 × 8.0; 29.0 × 9.0), non-ovigerous ♀ (26.0 × 10.0; 27.0 × 9.5; 27.5 × 9.5; 29.5 × 9.0; 29.5 × 9.0; 34.0 × 13.0; 35.5 × 14.0; 39.0 × 13.0; 42 × 18; 43.5 × 16.5; 44.5 × 20.5; 51.5 × 23.0), oviger-ous ♀ (28.0 × 12.0; 29.0 × 11.0; 34.5 × 13.0; 34.5 × 15.0; 36.0 × 16.0; 37.5 × 15.0; 38.0 × 15.0; 38.0 × 15.5; 38.0 × 15.5; 39.0 × 14.0; 40.0 × 15.0; 40.0 × 15.0; 41.0 × 15.0; 43.0 × 18.5; 43.5 × 17.0; 47.0 × 19.0; 50.5 × 25.0), damaged ♀ (27.0 × 9.5; 29.5 × 12.0; 32.0 × 13; 37.0 × 14.0; 37.0 × 14.0; 37.0 × 17.0; 37.5 × 14.0; 40.0 × 18.0). All specimens collected by R. L. Welicky, O. Kudlai, A. Greyling, D. van Rooyen, T. Beukes and Two Oceans Aquarium personnel on 28–29 April 2017 on the host P. blochii.
Additional museum material examined: Collected
by UCT Ecological Survey from Table Bay on host P. blochii and held at SAM as SAMA44882: ♀ (44.0 × 18.0; 44.0 × 20.0; 47.0 × 19.0; 47.0 × 21.0; 49.0 × 21.0; 50.0 × 22.0; 53.0 × 22.0). Collected from SAM and not catalogued: ♀ (45.0 × 22.0; 49.0 × 19.0; 49.0 × 20.0; 50.0 × 20.0; 50.0 × 22.0).
Representative DNA sequences: MK450443-MK450452
Description
Female lectotype. [BMNH 1979.329:207b; Fig. 1.] Size 43.0 × 18.0. Body ovoid, 2.6 times as long as greatest width; dorsal surfaces smooth and polished in appear-ance; widest at pereonite 5, most narrow at pereonite 1; pereonite lateral margins mostly posteriorly ovate. Cephalon 0.6 as long as wide as measured in dorsal view, trapezoid-shaped. Pereonite 1 smooth, anterior border straight, anterolateral angle narrowly rounded, in line with base of cephalon. Posterior margins of pereonites smooth, slightly curved laterally. Coxae 2–3 wide, with posteroventral angles rounded; coxae 4–7 with rounded point, curved, not extending past posterior pereonite margin; coxa 7 extending past anterior pereonite margin, coxae 4–6 not extending past anterior pereonite margin. Pereonites 1–5 increasing in length and width; pere-onites 6–7 decreasing in length and width; perepere-onites 5 and 6 subequal, or pereonites 1–4 narrower. Pleon with pleonite 5 nearly widest, visible in dorsal view; posterior margin of pleonites 1–4 posteriorly concave, pleonite 5 posteriorly slightly concave to straight or smooth, mostly concave. Pleonite 2 not laterally overlapped by pereonite 7; posterolateral angles of pleonite 2 narrowly rounded. Pleonite 1 similar in form to pleonite 2 and 3. Pleonites 3–5 similar in form to pleonite 2; pleonite 5 widest or nearly widest to pleonite 4, not overlapped by lateral margins of pleonite 4 or with posterolateral angles nar-rowly rounded, posterior margin straight. Pleotelson 0.8 times as long as anterior width, dorsal surface smooth; lateral margins convex, posterior margin evenly rounded.
Antennula approximately as stout as antenna, com-prised of 8 articles; peduncle articles 1 and 2 distinct, articulated. Antenna comprised of 8 articles.
Uropod more than half the length of pleotelson, pedun-cle 0.4 times as long as rami, pedunpedun-cle lateral margin without setae or without medial short acute robust seta; rami extending beyond pleotelson, marginal setae absent, apices broadly rounded. Endopod apically rounded, 3.6
times as long as greatest width, lateral margin weakly convex, medial margin weakly convex. Exopod extend-ing beyond end of endopod, 7.2 times as long as greatest width, apically rounded, lateral margin weakly convex, medial margin weakly convex, terminating without setae.
Female paralectotype. [BMNH 1979.329:207c; Fig. 1.] Size 46.0 × 19.0. Body ovoid, 2.6 times as long as great-est width; dorsal surfaces smooth and polished in appearance; widest at pereonite 5, most narrow at
pereonite 1; pereonite lateral margins mostly posteri-orly ovate. Cephalon 0.32 as long as wide as measured in dorsal view, trapezoid-shaped. Eyes oval, with distinct margins. Pereonite 1 smooth, anterior border straight, anterolateral angle narrowly rounded, in line with base of cephalon. Posterior margins of pereonites smooth and slightly curved laterally. Coxae 2–3 wide; with pos-teroventral angles rounded; coxae 4–7 with rounded point and curved; not extending past posterior pere-onite margin, coxa 7 extending past anterior perepere-onite
a b c d e f g h i j
Fig. 1 Anilocra capensis ♀ (a–e 43.0 × 18.0, BMNH 1979.329:207b; f–j 46.0 × 19.5, BMNH 1979.329:207c). a Dorsal view. b Lateral view. c Pleotelson. d Dorsal view of cephalon. e Ventral view of cephalon. f Dorsal view. g Lateral view. h Pleotelson. i Dorsal view of cephalon. j Ventral view of cephalon
margin, coxae 4–6 not extending past anterior pere-onite margin. Perepere-onites 1–5 increasing in length and width; pereonites 6–7 decreasing in length and width; pereonites 5 and 6 subequal, pereonites 1–4 narrower. Pleon with pleonite 1 wider than pleonites 2–5, visible in dorsal view; posterior margin of pleonites 1–4 poste-riorly concave; pleonite 5 posteposte-riorly slightly concave to straight or smooth, mostly concave. Pleonite 2 not over-lapped by pereonite 7; posterolateral angles of pleonite 2 narrowly rounded. Pleonite 1 similar in form to ple-onites 2 and 3. Pleple-onites 3–5 similar in form to pleonite 2; pleonite 5 equal in width to pleonite 4 or free, not overlapped by lateral margins of pleonite 4, pleonite 5 with posterolateral angles narrowly rounded, posterior margin straight. Pleotelson 0.9 times as long as anterior width, dorsal surface smooth, lateral margins convex, posterior margin evenly rounded.
Antennula approximately as stout as antenna; peduncle articles 1 and 2 distinct and articulated.
Uropod more than half the length of pleotelson, pedun-cle 0.7 times as long as rami, pedunpedun-cle lateral margin without setae; rami extending beyond pleotelson, mar-ginal setae absent, apices broadly rounded. Endopod api-cally rounded, 3.8 times as long as greatest width, lateral margin weakly convex, medial margin weakly convex. Exopod extending beyond end of endopod, 7 times as long as greatest width, apically rounded, lateral margin weakly convex, medial margin weakly convex, terminat-ing without setae.
Male paralectotype. [BMNH 1979.329:207a; Fig. 2.] Size 35.0 × 10.0. Body similar to female but smaller and
narrower; 3.5 times as long as wide. Pleopod 2 appendix masculina distally narrowly rounded.
Female. [New material SAMC-A091297; Figs. 3, 4.] Size 45.0 × 22.0. Body ovoid, 2.4 times as long as greatest width; dorsal surfaces smooth and polished in appear-ance; widest at pereonite 5, most narrow at pereonite 1; pereonite lateral margins mostly posteriorly ovate. Ceph-alon 0.42 as long as wide as measured in dorsal view, trapezoid-shaped. Eyes oval, with distinct margins, one eye width 0.2 times width of cephalon, one eye length 0.4 times length of cephalon. Pereonite 1 smooth, anterior border straight, anterolateral angle narrowly rounded, in line with base of cephalon. Posterior margins of pere-onites smooth, slightly curved laterally. Coxae 2–3 wide, with posteroventral angles rounded; coxae 4–7 with rounded point and curved; coxae 4–6 not extending past posterior pereonite margin; coxa 7 extending past rior pereonite margin; coxae 4–6 not extending past ante-rior pereonite margin. Pereonites 1–5 increasing in length and width; pereonites 6–7 decreasing in length and width; pereonites 5 and 6 subequal, pereonites 1–4 narrower. Pleon with pleonite 1 wider than pleonites 2–5, visible in dorsal view; posterior margin of pleonites 1–4 posteriorly concave, pleonite 5 posteriorly slightly concave to straight or smooth, mostly concave. Pleonite 2 not overlapped by pereonite 7; posterolateral angles of pleonite 2 narrowly rounded. Pleonite 1 similar in form to pleonites 2 and 3. Pleonites 3–5 similar in form to pleonite 2; pleonite 5 equal width to pleonite 4 or free, not overlapped by lateral margins of pleonite 4, with posterolateral angles narrowly rounded, posterior margin straight. Pleotelson 0.8 times
a b
c
d
e
Fig. 2 Anilocra capensis ♂ (35.0 × 10.0, BMNH 1979.329:207a). a Dorsal view. b Lateral view. c Pleotelson. d Dorsal view of cephalon. e Ventral view of cephalon
i h g f e a b c d
Fig. 3 Anilocra capensis ♀ (50.0 × 22.0, SAMC-A091297). a Dorsal view. b Lateral view. c Dorsal view of cephalon. d Ventral view of cephalon. e Pereopod 1. f Pereopod 2. g Pereopods 3. h Pereopod 6. i Pleotelson
as long as anterior width, dorsal surface smooth, lateral margins convex, posterior margin evenly rounded.
Antennula approximately as stout as antenna, com-prised of 8 articles; peduncle articles 1 and 2 distinct, articulated; article 2 0.8 times as long as article 1; arti-cle 3 0.7 times as long as wide, 0.4 times as long as com-bined lengths of articles 1 and 2; antennula flagellum
with 5 articles, extending to middle of eye; peduncle article 3 0.8 times as long as wide, article 2 with plu-mose setae, or articles 3 and 6 with robust setae. Antenna comprised of 8 articles, peduncle article 3 1.4 times as long as article 2, article 4 1.3 times as long as wide, article 5 1.5 times as long as wide, 1.0 times as long as article 4; antenna flagellum with 3 articles,
a b c d e f g h i j k l m n o p q r s a t u
Fig. 4 Anilocra capensis ♀ (45.0 × 22.0, SAMC-A091297). a–d Antenna. a Antenna. b Article 6 robust setae. c Article 7 robust setae. d Article 8 simple setae. e–h Antennula. e Antennula. f Plumose setae article 2. g Robust setae article 3. h Robust setae article 6. i Maxillule. j Maxillule apex. k Mandible. l Article 3 mandibular palp. m Maxilla. n Maxilla apex. o Maxilliped. p Article 3 of maxilliped. q–u Pleopods 1–5, respectively
terminal article terminating in 1–5 short simple setae, extending to middle of pereonite 1. Mandibular molar process ending in an acute incisor, with 23 simple setae. Maxillula simple, with 4 terminal robust setae. Max-illa medial lobe partly fused to lateral lobe; 2 recurved robust setae; and 2 large recurved robust setae. Maxil-liped weakly segmented, with lamellar oostegite lobe or second, smaller oostegite lobe on basal part of article, article 3 with 3 recurved robust setae.
Pereopod 1 basis 0.4 times as long as greatest width; ischium 1.9 times as long as basis; merus proximal mar-gin without bulbous protrusion; carpus with straight proximal margin; propodus 2.0 times as long as wide; dactylus stout, 2.4 times as long as basal width. Pereopod 2 propodus 1.8 as long as wide; dactylus 1.3 as long as propodus. Pereopods gradually increasing in size towards posterior. Pereopod 6 basis 2.6 times as long as greatest width, ischium 0.4 times as long as basis, propodus 1.3 as long as wide, dactylus 1.4 as long as propodus. Pereopod 7 basis 2.6 times as long as greatest width; ischium 0.6 as long as basis, without protrusions; merus 1.1 times as long as wide, 0.5 as long as ischium; carpus 1.3 times as long as wide, 1 as long as ischium, without bulbous pro-trusion; propodus 2.4 times as long as wide, 0.7 as long as ischium; dactylus moderately slender, 1.1 times as long as propodus, 2.9 times as long as basal width.
Pleopods without setae or lobes, exopod larger than endopod. Pleopod 1 exopod 1.3 times as long as wide, lateral margin weakly convex, distally narrowly rounded, medial margin weakly oblique, medial margin weakly convex; endopod 1.2 times as long as wide, lateral margin weakly convex, distally narrowly rounded, medial mar-gin slightly convex, peduncle 4.5 times as wide as long, without retinaculae. Pleopods 2–5 similar to pleopod 1. Pleopods 3–5 endopods proximal borders do not extend below exopod to peduncle. Peduncle lobes absent on ple-opods 1–3, or present, increasing on pleple-opods 4–5.
Uropod more than half the length of pleotelson, pedun-cle 0.5 times as long as rami, pedunpedun-cle lateral margin without setae or without medial short acute robust seta; rami extending beyond pleotelson, marginal setae absent, apices broadly rounded. Endopod apically rounded, 3.5 times as long as greatest width, lateral margin weakly convex, medial margin weakly convex. Exopod extend-ing beyond end of endopod, 6 times as long as greatest width, apically rounded, lateral margin weakly convex, medial margin weakly convex, terminating without setae.
Male. [New material SAMC-A091297; Figs. 5, 6.] Size 28.0 × 8.0. Body similar to females but much smaller and narrower; 3.1 times as long as wide. Pleopod 2 appen-dix masculina with parallel margins, 0.9 times as long as endopod, distally narrowly rounded.
Remarks
Anilocra capensis has a proportionally large ovoid and vaulted body with distinctly flexed distolateral mar-gins of the pereonite 7. The pleotelson is wider than long and broadly rounded and the distal-most article of the antenna peduncle has one convex lateral mar-gin. This species has the greatest range in proportional size of adult females and shows the most intrinsic vari-ation with regards to pleonite width and uropod length. This was evident in the syntypes as well as the new fresh material examined. The freshly fixed specimens can be grouped mainly into two categories: specimens where pleonite 3 is most narrow and 5 is widest, and specimens where pleonite 1 and 2 are widest. Within each of these groups, proportional size and uropod length is variable, but no general differences in variability can be discerned between the two groups. There also appears to be col-our variation (dark brown to red-brown, or tan) in A. capensis, but all the other characters to this species are observed in both colour types. Given that varying pleon morphology is the only character that can create groups within A. capensis, this is not sufficient to determine that there are two species.
In the present study Anilocra capensis was found attached to the skin of host fish. It was situated below the anterior portion of the dorsal fin, facing anteriorly, and above and not blocking the operculum. This attach-ment site was consistent among all the freshly collected material.
Anilocra capensis can be differentiated from the other species described below based on body, pereonite, pereo-pod and/or antenna/antennule form. The body shape of A. capensis is not twisted, whereas Anilocra jovanasi n. sp. and Anilocra hadfieldae n. sp. have weakly twisted bodies. Compared to A. capensis, Anilocra paulsikkeli n. sp. is 0.86 times as wide at the widest point and has nota-ble sub-parallel body margins, and Anilocra ianhudsoni n. sp. is strongly narrowed anteriorly. Anilocra capensis can be distinguished from Anilocra bunkleywilliamsae n. sp., as the latter is proportionally shorter, pereonite 1 is longer, the antenna and antennule are narrower, and it has chromatophores. Anilocra capensis can be differen-tiated from Anilocra angeladaviesae n. sp., as A. capen-sis has a pronounced curvature on the lateral margin of the propodus of pereopod 6 that A. angeladaviesae n. sp. lacks. There is an elongated lateral margin of article 3 of the antenna of A. angeladaviesae n. sp. that extends onto article 4, and this does not occur in A. capensis.
We note that the 200-year-old female paralectotypes were similar in shape and form to the fresh material, but their poor and fragile condition, along with miss-ing some articles made it possible to only include a few reliable drawings without further damage. The male
a b c d f g h i e
Fig. 5 Anilocra capensis ♂ (28.0 × 8.0, SAMC-A091297). a Dorsal view. b Lateral view. c Pleotelson. d Dorsal view of cephalon. e Ventral view of cephalon. f Pereopod 1. g Pereopod 2. h Pereopod 6. i Pereopod 7
paralectotype examined was significantly larger and likely in a different and later developmental stage than the freshly collected males. There was consistency in size and form of the fresh male material examined, but during our collections and those loaned from SAM, we found no male similar in size to Leach’s specimen.
It is important to note that the type-locality for A. capensis is Cape Town, South Africa, and the host is a sparid. Species of Anilocra are regarded as typically host specific to the family or genus level, and some even to the species level. Given this specificity and the general spatial distribution of A. capensis [31], it
a b c d e f g h i j k l m n o
Fig. 6 Anilocra capensis ♂ (28.0 × 8.0, SAMC-A091297). a Antenna. b Antennula. c Mandible. d Article 3 mandibular palp. e Maxilla. f Maxilla apex. g Maxilliped. h Article 3 of maxilliped. i Maxillule. j Maxillule apex. k–o Pleopods 1–5, respectively
is unlikely that the records from the North Atlantic or other regions are of A. capensis. Thus, the reports of A. capensis from the Canary Islands and from hosts repre-senting more than five families of fish are dubious [32,
33].
Anilocra ianhudsoni n. sp. Type-host: Unknown.
Type-locality: Nahoon River, East London, South
Africa.
Type-material: Holotype: ♀ (18.0 × 8.0), paratypes: ♀
(18.0 × 8.0, dissected), ♂ (11.5 × 3.0, dissected) (SAMC-A091293). Collected by personnel on the ‘SS Pieter Faure’.
Other-material: Transitional (11.5 × 3.0) (SAMC- A091293).
ZooBank registration: The Life Science Identifier
(LSID) for Anilocra ianhudsoni n. sp. is urn:lsid:zoobank. org:act:7AF506D2-5DD9-4B4A-8965-B1D224DE3650.
Etymology: This species is named for the first authorʼs
(RLW) nephew, Ian Hudson Catalano, as a gesture of her appreciation to him and his mother, LM Catalano, for understanding the importance of her living abroad to conduct cymothoid-related research and to drive Ian’s curiosity in the natural sciences.
Description
Female. [SAMC-A091293; Figs. 7, 8.] Size 18.0 × 8.0. Body diamond-shaped or ovoid, 2.6 times as long as great-est width; dorsal surfaces smooth and polished in appear-ance; widest at pereonite 5, most narrow at pereonite 1; pereonite lateral margins posteriorly protruding. Cepha-lon 0.9 as Cepha-long as wide as measured in dorsal view, sub-triangular. Eyes oval, with distinct margins, one eye width 0.3 times width of cephalon, one eye length 0.4 times length of cephalon. Pereonite 1 smooth, anterior border straight, anterolateral angle narrowly-rounded, in line with base of the cephalon. Posterior margins of pereonites smooth, slightly curved laterally. Coxae 2–3 wide, with posteroventral angles rounded; coxae 4–7 with rounded point and curved, extending past pereonite anterior mar-gin. Pereonites 1–5 increasing in length and width; pere-onites 6–7 decreasing in length and width; perepere-onites 5 and 6 subequal, pereonites 1–4 narrower, becoming more progressively rounded posteriorly. Pleon with pleonite 1 wider than pleonites 2–4, visible in dorsal view; posterior margin of pleonites 1–5 posteriorly concave, mostly con-cave. Pleonite 2 not overlapped by pereonite 7; postero-lateral angles of pleonite 2 narrowly-rounded. Pleonite 1 similar in form to pleonites 2 and 3. Pleonites 3–5 similar in form to pleonite 2; pleonite 5 free, not overlapped by lateral margins of pleonite 4, posterior margin straight.
Pleotelson 0.9 times as long as anterior width, dorsal surface smooth, lateral margins convex, posterior margin converging to weak caudomedial point.
Antennula more stout than antenna; comprised of 7 articles; peduncle articles 1 and 2 distinct, articulated; article 2 0.6 times as long as article 1; article 3 1 times as long as wide, 0.5 times as long as combined lengths of articles 1 and 2; flagellum with 4 articles, extend-ing to middle of eye, terminal article with 1 plumose seta. Antenna comprised of 9 articles; peduncle article 3 1.4 times as long as article 2; article 4 1.5 times as long as wide, 1.1 times as long as article 3; article 5 1.5 times as long as wide, 1.2 times as long as article 4; flagellum with 4 articles, terminal article terminating in 1–5 short simple setae, extending to middle of pereonite 1. Man-dibular molar process ending in an acute incisor, with 13 simple setae. Maxillula simple with 4 terminal robust setae. Maxilla medial lobe partly fused to lateral lobe; 2 recurved robust setae; and 2 large recurved robust setae. Maxilliped weakly segmented, with lamellar oostegite lobe or second, smaller oostegite lobe on basal part of article, palp article 2 with 0 simple setae, article 3 with 3 recurved robust setae.
Pereopod 1 basis 1.7 times as long as greatest width; ischium 0.7 times as long as basis; merus proximal mar-gin without bulbous protrusion; carpus with straight proximal margin; propodus 1.8 times as long as wide; dactylus moderately slender, 1.2 as long as propodus, 2.7 times as long as basal width. Pereopod 2 propodus 1.2 as long as wide; dactylus 1.9 as long as propodus. Pereopods gradually increasing in size towards posterior. Pereopod 6 basis 2.3 times as long as greatest width, ischium 0.5 times as long as basis, propodus 1.2 as long as wide, dac-tylus 1.8 as long as propodus. Pereopod 7 basis 2.8 times as long as greatest width; ischium 0.6 as long as basis, without protrusions; merus proximal margin without bulbous protrusion, merus 1.5 times as long as wide, 0.6 as long as ischium; carpus 1.7 times as long as wide, 0.5 as long as ischium, without bulbous protrusion; propo-dus 3.1 times as long as wide, 0.9 as long as ischium; dac-tylus slender, 1.0 as long as propodus, 4.5 times as long as basal width.
Pleopods without setae, exopod larger than endopod. Pleopod 1 exopod 0.7 times as long as wide, lateral mar-gin weakly convex, distally narrowly rounded, medial margin weakly oblique, medial margin weakly convex; endopod 0.5 times as long as wide, lateral margin weakly convex, distally narrowly rounded, medial margin slightly convex, without retinaculae. Pleopods 2–5 similar to ple-opod 1. Pleple-opods 3–5 endple-opods proximal borders do not extend below exopod to peduncle. Peduncle lobes absent.
Uropod longer than pleotelson, peduncle 0.5 times as long as rami, peduncle lateral margin without setae; rami
extending beyond pleotelson, marginal setae absent, api-ces narrowly rounded. Endopod apically rounded, 3.0 times as long as greatest width, lateral margin weakly convex, medial margin weakly convex. Exopod extending
beyond end of endopod, 6.0 times as long as greatest width, apically rounded, lateral margin weakly convex, medial margin weakly convex, terminating without setae.
a b c d e f g h i
Fig. 7 Anilocra ianhudsoni n. sp. ♀ (18.0 × 8.0, holotype SAMC-A091293). a Dorsal view. b Lateral view. c Dorsal view of cephalon. d Ventral view of cephalon. e Pleotelson (18.0 × 8.0, paratype SAM A6296). f Pereopod 1. g Pereopod 2. h Pereopod 6. i Pereopod 7
a b c d e f g h i j k l m n o p q r
Fig. 8 Anilocra ianhudsoni n. sp. ♀ (18.0 × 8.0, paratype SAMC-A091293). a Antennula. b Plumose setae of antennula terminal article. c Antenna. d Forked setae of antenna article 4. e Antenna terminal article. f Maxiliped. g Article 3 of maxiliped. h Maxillule apex. i Maxillule. j Maxilla. k Maxilla apex. l Mandible. m Article 3 of mandible. n–r Pleopods 1–5, respectively
Male. [SAMC-A091293; Fig. 9.] Size 11.5 × 3.0. Smaller than female. Body rectangular, 3.4 times as long as wide. Pleopod 2 appendix masculina with parallel margins, dis-tally narrowly rounded.
Remarks
Anilocra ianhudsoni n. sp. is among the smaller of the Anilocra species that occur in South Africa. The body shape is unique as it has a diamond-triangulate shape, such that pereonites 1–3 narrow strongly. Unlike the other African species, A. ianhudsoni n. sp. has no flexed pereonite posterolateral margins. The antennula has a plumose seta on the terminal article and a maxilla with simple setae or 3 recurved robust setae. Anilocra ianhud-soni n. sp. also has more slender pereopods and a flat-ter and broader antenna compared to the other species described herein.
Anilocra ianhudsoni n. sp. has a produced curvature on the lateral margin of the propodus of pereopod 6, which is similar to A. capensis and A. bunkleywilliamsae n. sp. Compared to Anilocra ianhudsoni n. sp., A. capensis and A. bunkleywilliamsae n. sp. both have broader, more ovoid pereonites, and a pleotelson that is closer in width to total body width.
Anilocra bunkleywilliamsae n. sp.
Type-host: Rhabdosargus holubi Steindachner, 1881. Type-locality: Mawalana Estuary, South Africa.
Type-material: Holotype ♀ (24.0 × 10.0) paratype ♀
(27.0 × 12.0, dissected) (SAMC-A091294). Collected by T. Mqolombia, on 17 February 1998.
Other material: Transitional (18.0 × 5.0).
ZooBank registration: The Life Science Identifier (LSID)
for Anilocra bunkleywilliamsae n. sp. is urn:lsid:zoobank. org:act:9DF125DA-624A-4712-BA07-D45CF4BC7C8F.
Etymology: This species is named in honour of Dr Lucy
Bunkley-Williams, a world expert on Anilocra who described nearly all of the known species of Anilocra from the Caribbean.
Description
Female. [SAMC-A091294; Figs. 10, 11.] Size 27.0 × 12.0. Body 2.2 times as long as greatest width with dorsal sur-faces smooth and polished in appearance. Body widest at pereonite 5, most narrow at pereonite 1, and pereonite lateral margins posteriorly protruding. Cephalon 0.8 as long as wide as measured in dorsal view, trapezoid-shaped. Eyes oval, with distinct margins, one eye width 0.2 times width of cephalon, one eye length 0.4 times length of cephalon. Pereonite 1 in line with base of cephalon, smooth, anterior border straight, anterolateral angle nar-rowly rounded. Posterior margins of pereonites smooth,
straight. Coxae 2–3 narrow, with posteroventral angles rounded; coxae 4–7 with rounded point, not extending past posterior pereonite margin. Pereonites 1–5 increas-ing in length and width; pereonites 6–7 decreasincreas-ing in length and width; pereonites 4–6 subequal, pereonites 1–3 subequal, increasing in similarity to pereonites 4–6. Pleon with pleonite 1 wider than pleonites 2–5, visible in dorsal view; posterior margin of pleonites 1–4 posteriorly concave, pleonite 5 posteriorly slightly concave to straight, mostly concave. Pleonite 2 not overlapped by pereonite 7; posterolateral angles of pleonite 2 narrowly rounded. Pleonite 1 similar in form to pleonites 2 and 3. Pleonites 3–5 similar in form to pleonite 2; pleonite 5 free, not over-lapped by lateral margins of pleonite 4, posterior margin straight. Pleotelson 1 times as long as anterior width. Dor-sal surface smooth. Pleotelson lateral margins convex, posterior margin converging to weak caudomedial point.
Antennula more stout than antenna; peduncle articles 1 and 2 distinct and articulated; article 2 0.8 times as long as article 1; article 3 1.0 times as long as wide, 0.4 times as long as combined lengths of articles 1 and 2; flagellum with 4 articles, extending to middle of eye, articles 3 and 6–8 with robust setae. Antenna comprised of 9 articles; peduncle article 3 1 times as long as article 2; article 4 0.7 times as long as wide, 0.9 times as long as article 3; article 5 1.6 times as long as wide, 1.5 times as long as article 4; flagellum with 4 articles, terminal article termi-nating in 6–10 short simple setae, extending to middle of pereonite 1. Mandibular molar process ending in an acute incisor, with 17 simple setae. Maxillula simple, with 4 terminal robust setae. Maxilla medial lobe partly fused to lateral lobe; lateral lobe with 0 simple setae, 2 recurved robust setae; medial lobe with 0 simple setae, and 2 large recurved robust setae. Maxilliped weakly segmented, with lamellar oostegite lobe or second, smaller oostegite lobe on basal part of article, palp article 2 with 0 simple setae, article 3 with 4 recurved robust setae. Oostegites margin covered in numerous plumose setae.
Pereopod 1 basis 1.7 times as long as greatest width; ischium 0.7 times as long as basis; merus proximal mar-gin without bulbous protrusion; carpus with straight proximal margin; propodus 1.2 times as long as wide; dactylus moderately slender, 1.2 as long as propodus, 2.6 times as long as basal width. Pereopod 2 propodus 1.9 as long as wide; dactylus 1.1 as long as propodus. Pereopods gradually increasing in size towards posterior. Pereopod 6 basis 2.5 times as long as greatest width, ischium 0.4 times as long as basis, propodus 1.3 as long as wide, dac-tylus 1.4 as long as propodus. Pereopod 7 basis 2.8 times as long as greatest width; ischium 0.5 as long as basis, without protrusions; merus proximal margin without bulbous protrusion, merus 1.6 times as long as wide, 0.6 as long as ischium; carpus 2.0 times as long as wide, 0.6
a b c d e f g h i j k
Fig. 9 Anilocra ianhudsoni n. sp. ♂ (11.5 × 3.0, SAMC-A091293). a Dorsal view. b Lateral view. c Pleotelson. d Antenna. e Antennule. f Dorsal view of cephalon. g Ventral view of cephalon. h Pereopod 1. i Pereopod 2. j Pereopod 6. k Pereopod 7
a b c d e f g h i
Fig. 10 Anilocra bunkleywilliamsae n. sp. ♀ (24.0 × 10.0, holotype, SAMC-A091294). a Dorsal view. b Lateral view. c Dorsal view of cephalon. d Ventral view of cephalon. e Pleotelson (27.0 × 12.0, paratype SAMC-A091294). f Pereopod 7. g Pereopod 6. h Pereopod 2. i Pereopod 1
a b c d e f g h i j k l m n o p q r s t u v w x y
Fig. 11 Anilocra bunkleywilliamsae n. sp. ♀ (27.0 × 12.0, paratype SAMC-A091294). a–e Antennula. a Antennula. b Setae of article 3. c–e Setae of articles 6–8, respectively. f Antenna. g–l Setae of antenna articles 4–9, respectively. m Maxilliped. n Article 3 of maxiliped. o Maxillule. p Maxillule apex. q Maxilla. r Maxilla apex. s Mandible. t Article 3 mandibular palp. u–y Pleopods 1–5, respectively
as long as ischium, without bulbous protrusion; propo-dus 2.8 times as long as wide, 1 as long as ischium; dac-tylus slender, 1 as long as propodus, 3.6 times as long as basal width. Dense chromatophores present on 1–7.
Exopod larger than endopod. Pleopod 1 exopod 1.4 times as long as wide, lateral margin weakly convex, distally nar-rowly rounded, medial margin weakly oblique, medial margin weakly convex; endopod 1.6 times as long as wide, lateral margin weakly convex, distally narrowly rounded, medial margin slightly convex, peduncle 2.9 times as wide as long, without retinaculae. Pleopods 2–5 similar to ple-opod 1. Pleple-opods 3–5 endple-opods proximal borders do not extend below exopod to peduncle. Peduncle lobes absent.
Uropod more than half the length of pleotelson, pedun-cle 0.5 times as long as rami, pedunpedun-cle lateral margin without setae; rami extending beyond pleotelson, mar-ginal setae absent, apices narrowly rounded. Endopod apically rounded, 5 times as long as greatest width, lateral margin weakly convex, medial margin weakly convex, terminating with 0 setae. Exopod extending beyond end of endopod, 9.7 times as long as greatest width, apically rounded, lateral margin weakly convex, medial margin weakly convex, terminating without setae.
Remarks
Anilocra bunkleywilliamsae n. sp. is most readily distin-guished from all other African species as it has the most anteriorly rounded and arched rostrum, and more setae on the antennula and antenna of the species described herein. It also has large and dense chromatophores on its pereopods. The chromatophores are denser on the left side when in dorsal view.
Compared to A. capensis, the body of A. bunkleywil-liamsae n. sp. is more ovoid, and pereopod 2 has robust setae present. Compared to A. ianhudsoni n. sp., A. bunkleywilliamsae n. sp. is generally smaller, its pleo-telson converges more and is rounder, and its dactyls are shorter. The ovate and non-twisted body shape of A. bunkleywilliamsae n. sp. makes it distinguishable from A. paulsikkeli n. sp. which is long and narrow, and A. jovanasi n. sp. and A. hadfieldae n. sp. which are weakly twisted. Anilocra bunkleywilliamsae n. sp.is most similar in pereonite shape to A. angeladaviesae n. sp., but these species can be differentiated from each other as A. ange-ladaviesae n. sp. has no produced curvature on the lat-eral margin of the propodus of pereopod 6, its pereopods are more slender, and the general shape and form of the antenna and antennula are dissimilar.
Anilocra paulsikkeli n. sp. Type-host: Unknown.
Type-locality: Delagoa Bay, Mozambique.
Type-material: Holotype ♀ (32.0 × 8.5), paratypes: ♀
(33.0 × 9.0, dissected), ♂ (16.0 × 3.0, dissected; 18.0 × 4.0) (SAMC-A091295). Unknown collector.
ZooBank registration: The Life Science Identifier
(LSID) for Anilocra paulsikkeli n. sp. is urn:lsid:zoobank. org:act:AFEA08B1-D27B-49F6-A30E-B4E142EFA5D9.
Etymology: This species is named in honour of Dr Paul C.
Sikkel, the PhD supervisor of the first author (RLW) and research collaborator of the second author (NJS). Dr Sik-kel’s devotion to furthering our knowledge on host-para-site interactions, strengthening the relationships between scientists and non-scientists, and rigorously mentoring future fish-parasite ecologists is hereby acknowledged.
Description
Female. [SAMC-A091295; Figs. 12–13.] Size 33.0 × 9.0. Body rectangular or elongate, 3.7 times as long as great-est width, dorsal surfaces smooth and polished in appear-ance, widest at pereonite 5, most narrow at pereonite 1, pereonite lateral margins subparallel. Cephalon 0.73 as long as wide as measured in dorsal view, trapezoid-shaped. Eyes oval, with distinct margins, one eye width 0.2 times width of cephalon; one eye length 0.45 times length of cephalon. Pereonite 1 in line with base of cephalon, smooth, anterior border straight, anterolateral angle narrowly rounded. Posterior margins of pereonites smooth and straight. Coxae 2–3 wide; with posteroven-tral angles rounded; 4–7 with rounded point and curved; not extending past posterior pereonite margin. Pere-onites 1–4 increasing in length and width; perePere-onites 6–7 decreasing in length and width; pereonites 5 and 6 subequal. Pleon with pleonite 1 largely concealed by pereonite 7, visible in dorsal view; pleonites posterior margin 1–4 posteriorly concave, pleonite 5 posteriorly slightly concave to straight, mostly concave. Pleonite 2 not overlapped by pereonite 7; posterolateral angles of pleonite 2 expanded, posteriorly produced or rounded. Pleonite 1 differ in form to pleonite 2 and 3. Pleonite 5 free, not overlapped by lateral margins of pleonite 4, with posterolateral angles narrowly rounded, posterior margin straight. Pleotelson 1.31 times as long as anterior width. Dorsal surface smooth. Pleotelson lateral margins con-vex, posterior margin converging to weak caudomedial point.
Antennula approximately as stout as antenna, com-prised of 9 articles; peduncle articles 1 and 2 distinct and articulated; article 2 1.3 times as long as article 1; article 3 0.9 times as long as wide, 0.6 times as long as combined lengths of articles 1 and 2; flagellum with 5 articles, ter-minal article with robust and simple setae, extending to posterior margin of eye. Antenna comprised of 9 articles; peduncle article 3 1.0 times as long as article 2; article 4 1.15 times as long as wide, 1.5 times as long as article 3;
i h g f e a b c d
Fig. 12 Anilocra paulsikkeli n. sp. ♀ (a–e 32.0 × 8.5, holotype, SAMC-A091295; f–i 33.0 × 9.0, paratype, SAMC-A091295). a Dorsal view. b Lateral view. c Dorsal view of cephalon. d Ventral view of cephalon. e Pleotelson. f Pereopod 1. g Pereopod 2. h Pereopod 6. i Pereopod 7
article 5 1.4 times as long as wide, 1.2 times as long as article 4; flagellum with 4 articles, terminal article ter-minating in no setae, extending to middle of pereonite 1. Mandibular molar process ending in an acute incisor, with 11 simple setae. Maxillula simple, with 2 terminal robust setae. Maxilla medial lobe partly fused to lateral lobe; 2 recurved robust setae and 2 large recurved robust setae. Maxilliped weakly segmented, with lamellar oost-egite lobe or second, smaller oostoost-egite lobe on basal part
of article, article 3 with 3 recurved robust setae. Ooste-gites margin covered in numerous plumose setae.
Pereopod 1 basis 2.5 times as long as greatest width; ischium 0.5 times as long as basis; merus proximal mar-gin with slight bulbous protrusion; carpus with straight proximal margin; propodus 2.1 times as long as wide; dactylus stout, 1.2 as long as propodus, 3 times as long as basal width. Pereopod 2 propodus 2.5 as long as wide; dactylus 1.1 as long as propodus. Pereopods gradually increasing in size towards posterior. Pereopod 6 basis
a b c d e f g h i j k l m n o
Fig. 13 Anilocra paulsikkeli n. sp. ♀ (33.0 × 9.0, paratype, SAMC-A091295). a–b Antenna. a Antenna. b Setae of article 8. c–e Antennula. c Antennula. d Setae of article 3. e Setae of terminal article. f Mandible. g Article 3 mandibular palp. h Maxilliped. i Article 3 of maxilliped. j Pleopod 1. k Maxilla. l Maxilla apex. m Maxillule. n Maxillule apex. o Pleopod 2
2.7 times as long as greatest width, ischium 0.5 times as long as basis, propodus 2.6 as long as wide, dactylus 1 as long as propodus. Pereopod 7 basis 2.4 times as long as greatest width; ischium 0.7 as long as basis, without trusions; merus proximal margin without bulbous pro-trusion, merus 1.7 times as long as wide, 0.6 as long as ischium; carpus 1.5 times as long as wide, 0.44 as long as ischium, without bulbous protrusion; propodus 4.3 times as long as wide, 1.1 as long as ischium; dactylus slender, 0.9 as long as propodus, 4.4 times as long as basal width.
Pleopods without setae, exopod larger than endopod. Pleopod 1 exopod 2.1 times as long as wide, lateral mar-gin weakly convex, distally narrowly rounded, medial margin weakly oblique, medial margin weakly convex; endopod 2.2 times as long as wide, lateral margin weakly convex, distally narrowly rounded, medial margin slightly convex, peduncle 2.0 times as wide as long, without reti-naculae. Pleopods 3–5 endopods proximal borders do not extend below exopod to peduncle. Peduncle lobes absent. Uropod more than half the length of pleotelson, pedun-cle 0.7 times as long as rami, lateral margin without setae; rami extending to pleotelson apex, marginal setae absent, apices broadly rounded. Endopod apically rounded, 3.3 times as long as greatest width, lateral margin weakly convex, medial margin weakly convex, terminating with 0 setae. Exopod not extending to end of endopod, 6.7 times as long as greatest width, apically rounded, lateral margin weakly convex, medial margin weakly convex, terminat-ing without setae.
Male. [SAMC-A091295; Fig. 14.] Size 16.0 × 3.0. Simi-lar to female but much smaller. Body rectanguSimi-lar, weakly twisted, 4.9 times as long as wide. Pleopod 2 appendix masculina with parallel margins, 0.8 times as long as endopod, distally narrowly rounded.
Remarks
Anilocra paulsikkeli n. sp. has a distinctive elongate, nar-row body, with sub-parallel lateral margins. Pleonite 1 is partly covered by pereonite 7. The lateral margins of the pleotelson are sub-parallel and from the last third of the pleotelson there is a strong convergence to a rounded medial point. The eyes of A. paulsikkeli n. sp. are nearly one-quarter the width of the cephalon.
Anilocra paulsikkeli n. sp. is similar to A. leptosoma, but there are multiple differences. Anilocra paulsikkeli n. sp. is larger (3.7 times as long as wide whereas A. leptosoma is 3.4 times as long as wide), the first antenna article 3 is not produced, pleonite 1 is more concealed, and the posterior margin of the pleonites are straighter. Aneesh et al. [34] recently resdescribed A. leptosoma but did not examine the A. leptosoma lectotype material, and the drawings of Aneesh are in disagreement with Bruce
1987’s drawings of the lectotype. In Aneesh et al. [34], Fig. 1d, e appear to not resemble A. leptosoma, and are more similar to A. capensis, with respect to body shape and form in dorsal view. Aneesh et al. [34] likely did not identify A. leptosoma. To identify the species these authors report on, specimens should be further examined morphologically, and molecular data should be provided.
Anilocra paulsikkeli n. sp. is also similar to Anilocra caudata Bovallius, 1887 but can be distinguished from A. caudata, by the wider pleotelson, pleonite 1 not being covered by pereonite 7 and the lateral margins of pleonite 5 are more acute.
Compared to the other species described herein, A. paulsikkeli n. sp. and A. jovanasi n. sp. are most simi-lar. Both species have nodules that occur midway on the lateral margins of the dactyls of pereopods 1 and 2, but the nodules are larger on A. paulsikkeli n. sp. Anilocra jovanasi n. sp. also has antennula peduncle article 3 more strongly produced than that of A. paulsikkel n. sp.
Anilocra jovanasi n. sp. Type-host: Unknown.
Type-locality: Delagoa Bay, Mozambique.
Type-material: Holotype ♀ (20.0 × 7.0, dissected) (SAMC-A091296). Unknown collector.
ZooBank registration: The Life Science Identifier
(LSID) for Anilocra jovanasi n. sp. is urn:lsid:zoobank. org:act:DA044D6D-CA90-4E20-9D76-1D6FD636569C.
Etymology: This species is named for Professor Jo G. van
As (1949–2018), the late aquatic parasitologist and PhD advisor of the second author (NJS). Professor van As revived the field of aquatic parasitology in South Africa in the 1970s and all current active aquatic parasitologists in South Africa are either his former students, or a stu-dent of one of his former stustu-dents. His contribution to the field of aquatic parasitology in South Africa is hereby acknowledged.
Description
Female. [SAMC-A091296; Figs. 15, 16.] Size 20.0 × 7.0. Body ovoid and weakly twisted, 2.9 times as long as greatest width, dorsal surfaces smooth and polished in appearance, most narrow at pereonite 1, pereonite lateral margins mostly posteriorly ovate. Cephalon 0.67 as long as wide as measured in dorsal view, trapezoid-shaped. Eyes oval, with distinct margins, one eye width 0.2 times width of cephalon; one eye length 0.4 times length of cephalon. Pereonite 1 in line with base of cephalon, smooth, anterior border straight, anterolateral angle nar-rowly rounded. Posterior margins of pereonites smooth and straight, 5 extended posteriorly and overlay lateral
a b c d e f g h i j k l m
Fig. 14 Anilocra paulsikkeli n. sp. ♂ (16.0 × 3.0, SAMC-A091295). a Dorsal view. b Lateral view. c Pleotelson. d Antenna, damaged. e Antennula. f Dorsal view of cephalon. g Ventral view of cephalon. h Pleopod 1. i Pleopod 2. j Pereopod 1. k Pereopod 2. l Pereopod 6. m Pereopod 7
a b c d e f g h i
Fig. 15 Anilocra jovanasi n. sp. ♀ (20.0 × 7.0, holotype, SAMC-A091296). a Dorsal view. b Lateral view. c Dorsal view of cephalon. d Ventral view of cephalon. e Pleotelson. f Pereopod 1. g Pereopod 2. h Pereopod 6. i Pereopod 7
margins of pleotelson. Coxae 2–3 wide; with posteroven-tral angles rounded; coxae 4–7 with rounded point and curved; not extending past posterior pereonite margin. Pereonites 1–5 increasing in length and width; pereonites 6–7 decreasing in length and width; pereonites 5 and 6 subequal, pereonites 1–4 narrower. Pleon with pleonite 1 wider than 2–4, visible in dorsal view; posterior margin of pleonites 1–4 posteriorly concave, pleonite 5 posteri-orly slightly concave to straight, mostly concave. Pleonite 2 not overlapped by pereonite 7; posterolateral angles of pleonite 2 narrowly rounded. Pleonite 1 similar in form to pleonite 2. Pleonites 3–5 differ in form from pleonite
5; pleonite 5 free, not overlapped by lateral margins of pleonite 4, posterolateral margins extend onto anterior portion of pleotelson, posterior margin straight. Pleo-telson 1.3 times as long as anterior width, dorsal surface smooth, lateral margins weakly convex, posterior margin converging to weak caudomedial point.
Antennula approximately as stout as antenna; com-prised of 8 articles; peduncle articles 1 and 2 distinct and articulated; article 2 1.1 times as long as article 1; article 3 0.6 times as long as wide, 0.4 times as long as combined lengths of articles 1 and 2; flagellum with 5 articles, arti-cles 6–8 with robust setae, extending to middle of eye.
a b c d e f g h i j k l m n o p q r s t u
Fig. 16 Anilocra jovanasi n. sp. ♀ (20.0 × 7.0, holotype, SAMC-A091296). a–d Antenna. a Antenna. b–d Setae of articles 8–10, respectively. e–h Antennula. e Antennula. f–h Setae of articles 6–8, respectively. i Maxilla. j Maxilla apex. k Maxillule. l Maxillule apex. m Maxilliped. n Article 3 of maxilliped. o Mandible. p Mandibular palp. q–u Pleopods 1–5, respectively
Antenna comprised of 10 articles; peduncle article 3 1.7 times as long as article 2; article 4 1.4 times as long as wide, 1.4 times as long as article 3; article 5 2.6 times as long as wide, 1.5 times as long as article 4; flagellum with 5 articles, articles 8–9 with setae, terminal article termi-nating in 1–5 short simple setae, extending to middle of pereonite 1. Mandibular molar process present, with 20 simple setae. Maxilla medial lobe partly fused to lateral lobe; 2 recurved robust setae; and 2 large recurved robust setae. Maxilliped weakly segmented, with lamellar oost-egite lobe, palp article 2 with 0 simple setae, article 3 with 3 recurved robust setae. Oostegites margin covered in numerous plumose setae.
Pereopod 1 basis 2.7 times as long as greatest width; ischium 0.6 times as long as basis; merus proximal mar-gin without bulbous protrusion; carpus with straight proximal margin; propodus 2 times as long as wide; dac-tylus slender, 1.2 as long as propodus, 3.2 times as long as basal width. Pereopod 2 propodus 2.15 as long as wide; dactylus 1 as long as propodus. Pereopods gradu-ally increasing in size towards posterior. Pereopod 6 basis 2.91 times as long as greatest width, ischium 0.5 times as long as basis, propodus 2.6 as long as wide, dactylus 1.1 as long as propodus. Pereopod 7 basis 3.1 times as long as greatest width; ischium 0.6 as long as basis, without protrusions; merus proximal margin without bulbous protrusion, merus 2.0 times as long as wide, 0.7 as long as ischium; carpus 1.9 times as long as wide, 0.6 as long as ischium, without bulbous protrusion; propodus 3.2 times as long as wide, 0.9 as long as ischium; dactylus slender, 0.86 as long as propodus, 5.0 times as long as basal width.
Pleopods without setae, exopod larger than endopod. Pleopod 1 exopod 1.6 times as long as wide, lateral mar-gin weakly convex, distally narrowly rounded, medial margin weakly oblique, medial margin weakly convex; endopod 1.6 times as long as wide, lateral margin weakly convex, distally narrowly rounded, medial margin slightly convex, peduncle 2.4 times as wide as long, without reti-naculae. Pleopods 2–5 similar to pleopod 1. Pleopods 3–5 endopods proximal borders do not extend below exopod to peduncle. Peduncle lobes present on 5.
Uropod more than half the length of pleotelson, pedun-cle lateral margin without setae; rami not extending beyond pleotelson, marginal setae absent, apices nar-rowly rounded. Endopod apically rounded, lateral margin weakly convex, medial margin weakly convex. Apically rounded, lateral margin weakly convex, medial margin weakly convex.
Remarks
The body Anilocra jovanasi n. sp. is weakly twisted and narrowly ovoid. In dorsal view, the antennula appears geniculate due to the anterodistal lateral angle of the
third article; pereopods 6 and 7 possess few robust setae. Anilocra jovanasi n. sp. is the only species described in southern Africa with the posterolateral margins of the fifth pleonite extending onto the dorsal portion of the pleotelson.
Anilocra jovanasi n. sp. is most similar in body shape to A. leptosoma, and A. paulsikkeli n. sp. However, the body of A. leptosoma is not twisted and the pleotelson converges strongly to a distinct point, whereas in A. jovanasi n. sp., the body is straight and the pleotelson converges broadly to a very weak point. The pleotel-sons of A. paulsikkeli n. sp. and A. jovanasi n. sp. are similar in shape until the last third, at which point the lateral margins of the pleotelson of A. jovanasi n. sp. converge broadly to a very weak point, and those of A. paulsikkeli n. sp. converge strongly to a broad medial point. Moreover, pleonite 1 of A. jovanasi n. sp. is sub-stantially more visible than that of A. paulsikkeli n. sp. Whereas A. paulsikkeli n. sp. has distinct nodules on the dactyls of pereopods 1 and 2, this is present only on pereopod 1 of A. jovanasi n. sp. The propodus of pereopod 2 is without a curved produced curvature on the lateral margin, separating it from A. capensis and A. bunkleywilliamsae n. sp. Anilocra ianhudsoni n. sp. has a distinctly different body form and its pereopods 6 and 7 are more dense in robust setae. Anilocra ange-ladaviesae n. sp. is more robustly ovoid, with a broader pleotelson than A. jovanasi n. sp. Anilocra hadfieldae n. sp. also has a weakly twisted body, but compared to A. jovanasi n. sp., it has a more produced pointed lat-eral margin of pleonite 7 and a medial indent on the pleotelson.
Anilocra angeladaviesae n. sp.
Syn. Anilocra capensis Monod, 1924
Type-host: Uncertain (see Remarks). Type-locality: Cape Blanc, Morocco.
Type-material: Holotype ♀ (BMNH1924.5.30.6)
(37.0 × 18.0), paratypes (BMNH1924.5.30.7–10): ♀
(38.0 × 17.0; 37.0 × 15.0; 35.0 × 13.0), ♂ (24.0 × 6.0; 18.0 × 5.0). There are 6 accession numbers but 7 speci-mens in this lot. Collected by M. Theo Monod, 1923.
ZooBank registration: The Life Science Identifier (LSID)
for Anilocra angeladaviesae n. sp. is urn:lsid:zoobank. org:act:3381D22E-67B0-4847-A559-0899B20E9F7D.
Etymology: This species is named after Professor Angela
Josephine Davies (1947–2013), PhD promotor and post-doctoral advisor to the second author (NJS), to commem-orate her contribution to the knowledge of crustacean parasites of fishes, as well as her singular dedication to- and enthusiasm for sharing this knowledge with all those she mentored.
