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The handle http://hdl.handle.net/1887/20021 holds various files of this Leiden University dissertation.
Author: Lanz, Henriette Leonore
Title: Cancer-related targets sensed by apoptin Issue Date: 2012-10-24
Cancer-related targets sensed by apoptin
Henriëtte Leonore Lanz
The research presented in this thesis was performed at the department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands.
Cover: Fluorescent microscope picture of human osteasarcoma cells transiently transfected with plasmid encoding flag-apoptin. After fixation cells were stained for apoptin (red), tubulin (green) and DNA (blue).
© Henriette Lanz, The Netherlands, 2012
All rights reserved. No part of this thesis may be reproduced or transmitted in any form or by any means without written permission from the author.
An electronic version of this dissertation is available at the Leiden University Repository (https://openaccess.leidenuniv.nl)
Printed by Optima, Rotterdam, The Netherlands
Cancer-related targets sensed by apoptin
PROEFSCHRIFT
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus Prof. Mr. P.F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op woensdag 24 oktober 2012 klokke 15.00 uur
door
Henriëtte Leonore Lanz
geboren te Leidschendam in 1983
Promotiecommissie
Promotor Prof. Dr. M.H.M. Noteborn Copromotor Dr. C. Backendorf
Overige leden Prof. Dr. J. Brouwer
Prof. Dr. J.-P. Abrahams
Prof. Dr. M. Tavassoli (King’s College, London)
Prof. Dr. P.C.W. Hogendoorn
Contents
Thesis Outline 7
Introduction: Proteins Killing Tumor Cells 12
PP2A inactivation is a crucial step in triggering apoptin-induced tumor- selective cell killing
Cell Death and Disease (2012) 3, e291
37
Proteasomal insensitivity of apoptin in tumor cells
Biochemical and Biophysical Research Communications (2012) 422, 169-173
55
Development and application of an in vitro apoptin kinase assay Analytical Biochemistry (2012) 421, 68-74
67
Mitotic catastrophe triggered in human cancer cells by the viral protein apoptin
Manuscript submitted
83
Discussion: Proteins Killing Tumor Cells, Targetting the Hallmarks of Cancer 95
References 111
Samenvatting 127
Curriculum Vitae 131
List of Publications 133
