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University of Groningen Probiotic Bacteria and Their Encapsulation Evaluated in Advanced Co-culture Models Yuan, Lu

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University of Groningen

Probiotic Bacteria and Their Encapsulation Evaluated in Advanced Co-culture Models Yuan, Lu

DOI:

10.33612/diss.160691131

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2021

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Yuan, L. (2021). Probiotic Bacteria and Their Encapsulation Evaluated in Advanced Co-culture Models. University of Groningen. https://doi.org/10.33612/diss.160691131

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Probiotic Bacteria and Their Encapsulation

Evaluated in

Advanced Co-culture Models

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Probiotic bacteria and their encapsulation evaluated in advanced co-culture models

University Medical Center Groningen, University of Groningen Groningen, The Netherlands

Cover artwork: Lu Yuan Layout: Lu Yuan

Printing: Ipskamp Printing B.V. Copyright © 2021 by Lu Yuan

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Probiotic bacteria and their encapsulation

evaluated in

advanced co-culture models

PhD thesis

to obtain the degree of PhD at the University of Groningen

on the authority of the

Rector Magnificus Prof. C. Wijmenga and in accordance with

the decision by the College of Deans. This thesis will be defended in public on Monday 22 March, 2021 at 14.30 hours

by

Lu Yuan

born on 04 August 1990 in Sichuan, China

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Supervisors

Prof. H. C. van der Mei Prof. H. J. Busscher

Co-supervisors

Dr. B. W. Peterson

Assessment Committee

Prof. P. de Vos Prof. Y. Ren Prof. Y. Wei

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Paranimfen:

Huaiying Zhang Yangyang Guo

(8)

Table of Contents

Chapter 1 General Introduction and Aim of the

Thesis

1

Chapter 2 Two-stage Interpretation of Changes in

TEER of Intestinal Epithelial Layers

Protected by Adhering Bifidobacteria

during E. coli Challenges

Frontiers in Microbiology, 2020, 11: 599555

doi:10.3389/fmicb.2020.599555

15

Chapter 3 Visualization of Bacterial Colonization

and Cellular Layers in a Gut-on-a-Chip

System Using Optical Coherence

Tomography

Microscopy and Microanalysis 2020, 26: 1211-1219

doi:10.1017/S143192762002454X

47

Chapter 4 Escherichia coli Colonization of Intestinal

Epithelial Layers In Vitro in the Presence

of Encapsulated Bifidobacterium breve

for its Protection against

Gastro-intestinal Fluids and Antibiotics

Submitted to ACS Applied Materials & Interface, 2020

69

Chapter 5

General Discussion

95

Summary

102

Samenvatting

105

!"

109

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