University of Groningen
Ether-lipid membrane engineering of Escherichia coli
Caforio, Antonella
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2017
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Caforio, A. (2017). Ether-lipid membrane engineering of Escherichia coli. University of Groningen.
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Stellingen bij het proefschrift:
Ether-lipid membrane engineering of Escherichia coli
by Antonella Caforio
1. Every single step is a fundamental brick to complete the journey. Characterization of CarS and the polar head groups attachment enzymes was essential to reconstitute the ether lipid biosynthetic pathway (Chapter 2 & 3)
2. Dreams come true: if you dream about LCMS stopping during an overnight run, you better wake up and call maintenance
3. Enzymatic substrate promiscuity and chiral non-selectivity suggest the existence of ancient non-selective enzymes in the last universal ancestor cell (LUCA) (Chapter 3 & 4)
4. Everything in life is about equilibrium. Biology is essentially based on finding the proper balance so a cell can live
5. All living organism on Earth derive from a single common ancestor cell, whose membrane might be characterized by the coexistence of two different lipid species (Chapter 4)
6. Acronyms are not always the easiest way to name compounds (i.e. GGPP, GGGP, DGGGP)
7. Low sequence homology does not exclude structural conservation, as with the archaeal CarS and bacterial CdsA that despite poor overall sequence homology share a highly conserved CTP-binding pocket and catalytic core domain (Chapter 5)
8. Is not the strongest of the species that survive, nor the most intelligent, but the one most responsive to change. Charles Darwin 9. Above all, don’t fear difficult moments. The best comes from them.
