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University of Groningen

The consequences of environmental conditions for antagonistic pleiotropic effects of cellular

senescence

van Vliet, Thijmen

DOI:

10.33612/diss.156836397

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from

it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date:

2021

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

van Vliet, T. (2021). The consequences of environmental conditions for antagonistic pleiotropic effects of

cellular senescence. University of Groningen. https://doi.org/10.33612/diss.156836397

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Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.

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530, 184–189 (2016).

4. Franceschi, C. & Campisi, J. Chronic inflammation (Inflammaging) and its potential contribution to age-associated diseases. The Journals of Gerontology. Series A, Biological

Sciences and medical sciences 69, 4–9 (2014).

5. Baar, M. P. et al. Targeted Apoptosis of Senescent Cells Restores Tissue Homeostasis in Response to Chemotoxicity and Aging. Cell 169, 132-147 (2017).

6. Xu, M. et al. JAK inhibition alleviates the cellular senescence-associated secretory phenotype and frailty in old age. PNAS 112, 6301–6310 (2015)

7. Storer, M. et al. Senescence is a developmental mechanism that contributes to embryonic growth and patterning. Cell 155, 1119-1130 (2013).

8. Muñoz-Espín, D. et al. Programmed cell senescence during mammalian embryonic development. Cell 155, 1104-1118 (2013).

9. Krizhanovsky, V. et al. Senescence of Activated Stellate Cells Limits Liver Fibrosis. Cell 134,

657–667 (2008).

10. Demaria, M. et al. An essential role for senescent cells in optimal wound healing through secretion of PDGF-AA. Dev. Cell 31, 722–733 (2014)

11. Hunt, N. D. et al. Effect of calorie restriction and refeeding on skin wound healing in the rat.

Age (Omaha). 34, 1453–1458 (2012).

12. Fontana, L. & Partridge, L. Promoting health and longevity through diet: From model organisms to humans. Cell 161, 106–118 (2015).

13. Herranz N, Gallage S, Mellone M, Wuestefeld T, Klotz S, Hanley CJ, et al. mTOR regulates MAPKAPK2 translation to control the senescence-associated secretory phenotype, Nat Cell

Biol.17, 1205–1217 (2015).

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Acknowledgements

Thijmen van Vliet

European Research Institute for the Biology of Ageing (ERIBA), University Medical Center Groningen (UMCG), 9713AV Groningen, The Netherlands

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Completing my PhD would have never been possible without the help and support of many people. First of all, I would like to thank Marco, for the trust in me as the first master student in the lab and

later all the support during the PhD. Your true passion for science, your drive to work hard and your calmness has been inspiring to me and it helped me a lot to move my experiments forward. I really enjoyed the discussions during our 1-on-1 meetings, it always helped me to look at things from a different angle. Many thanks for your support in the past years on the personal and professional level. I also would like to thank Marcel and Ody. Even though you have very busy agenda’s you always

managed to be easily approachable and always responded quickly to my questions. Thank you for providing your valid feedback and helping me with issues I had during the PhD. Your view on my projects helped me to stay openminded and gave me more energy and enthusiasm to move forward.

Boshi (a.k.a the all-knowing professor Wang), my dear friend, thanks a lot for making me laugh so hard

when my projects were not running smoothly and my mood was bad. Your jokes truly lightened up my days and my PhD time wouldn’t have been as fun without you. I also really appreciate our honest discussions about each other’s data and our motto: garbage in garbage out! During these years you became such a close friend and I really appreciate that. Eleni (a.k.a fantastic labmate or professor

Greece), since the moment you entered the lab I felt we connected well which grew to a close friendship that I really appreciate. You humor contributed a lot to the nice working environment. Working together in the cell culture room made the, sometimes repetitive, work much more fun.

Jamil, I cannot remember how many nicknames I have for you. To name a few: Jamelt, Jamelto,

Jameltinio, Jamolt, Jamolta, professor bullshit, professor Lebanon and one that I don’t dare to write here. I very much enjoyed working with you. I will always remember your ever expanding and detailed scientific knowledge and your innate capacity to come up with new experiments. You are a very brave and strong person encountering unusual problems with regards to your home country. I hope we will share the Dutch nationality sometime soon. Simone, thanks a lot for your support during the master

internship and my PhD. I remember the days when we were the only people working in the lab listening to music in the cell culture room. We had so much fun together! Marta, buenas tardes and its almost

Friday!! Your smile and jokes always filled the lab with happiness which contributed a lot to a nice working environment. Jas, I really enjoyed working and writing with you and this sentence will always

stick in my mind when I don’t want to do a task: ahhg, I can’t be bothered… Sebastian, howdy!

Unfortunately we didn’t spend longer together in the lab but your passion for science and knowledge about many different topics impressed me from the beginning. I am sure you will have a bright future ahead and hope we can drink a beer together soon.

Nynke, your positive vibes, smiling face and willingness to help people are amazing! I always enjoyed

our small talk and discussions about the progression of our projects. I am sure you have a very bright

145

future with lots of fun, traveling and dancing ahead. Ale, I really appreciate your honest and

well-thought opinions about different aspects of life! I learned a lot from you and enjoyed working with you. You will certainly achieve whatever you want. Abel, your positive attitude and funny jokes made

me smile. Your quick way of thinking and connecting processes amazed me from the first moment I met you. Willem, you are probably one of the few people understanding the fun of renovation and

living in the countryside. Your calmness and motivation to do science inspired me. Lotte, first as a

master student and later as a colleague, I really like working with you. You are a smart and happy person and I am sure you will achieve what you want in the future! Michela, thanks a lot for teaching

me about Italian culture and your hard work during the revision of my oxygen manuscript. My students:

Carlos, Nikita and Sarina. I have seen you growing during your internships and you are all very nice

and smart people. Thank you for working hard and I wish you all the best for the future. I would also like to thank all the people working on the first floor, Cor, Christine, Gertrud, Hidde, Josi, Britt, Amr, Tobias, Ellen, Wytse, Rene, Leen, Ester, Mandy, Anita and all other colleagues from ERIBA. Thank you

for the nice working environment, the help during my projects and the interesting discussions. I really enjoyed working with all of you!

Marjolijn, mijn lieve vrouw, ik kan je niet genoeg bedanken voor je steun en doorzettingsvermogen in

de afgelopen jaren. Jij stond altijd achter mijn keuzes, zonder jou was dit nooit gelukt. Jouw rust en vertrouwen hebben me geholpen om door te zetten en me zelfvertrouwen te geven, zelfs toen ik het echt even niet meer zag zitten. Alles deed je zonder ook maar een keer te mopperen als ik weer op onmogelijke tijden naar het lab moest, te moe was of geen tijd had om aan ons huis te klussen of met mijn gedachten bij mijn experimenten zat. Jij steunde me altijd. Ik ben zo ontzettend trots op jou, op alles wat je doet maar vooral dat je bij mij hoort en dat we samen een team zijn. Ik kan niet wachten tot onze dochter geboren wordt en ik hou van je.

Tenslotte wil ik mijn familie bedanken voor de steun in de afgelopen jaren. Julie hebben ervoor gezorgd dat ik de veilige basis had waar ik op kon bouwen en terugvallen. Mam en Erwin, dank voor jullie hulp

tijdens lastige periodes. Even een bakje koffie, een rustig gesprek en de gezelligheid was wat ik soms echt nodig had om dingen op een rijtje te krijgen. Pap en Sieg, bedankt voor de motivatie om te geloven

in mezelf en de overtuiging dat ik kon worden wat ik wilde worden. Sjoerd en Siska, bedankt dat ik bij

jullie mocht wonen toen ik even geen (t)huis had. De warmte en liefde van jullie gezin en stabiele thuis waar ik in terecht kwam heeft me ontzettend geholpen om mezelf terug te vinden. En natuurlijk mijn broers en zus, Jochem, Daan en Annemieke. Jullie liefde, interesse, slechte grappen en gezelligheid

hebben me altijd geholpen om te relativeren en de moed erin te houden. Everybody, thank you and I hope to see you in the future!

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Processed on: 13-1-2021 PDF page: 145PDF page: 145PDF page: 145PDF page: 145

144

Completing my PhD would have never been possible without the help and support of many people. First of all, I would like to thank Marco, for the trust in me as the first master student in the lab and

later all the support during the PhD. Your true passion for science, your drive to work hard and your calmness has been inspiring to me and it helped me a lot to move my experiments forward. I really enjoyed the discussions during our 1-on-1 meetings, it always helped me to look at things from a different angle. Many thanks for your support in the past years on the personal and professional level. I also would like to thank Marcel and Ody. Even though you have very busy agenda’s you always

managed to be easily approachable and always responded quickly to my questions. Thank you for providing your valid feedback and helping me with issues I had during the PhD. Your view on my projects helped me to stay openminded and gave me more energy and enthusiasm to move forward.

Boshi (a.k.a the all-knowing professor Wang), my dear friend, thanks a lot for making me laugh so hard

when my projects were not running smoothly and my mood was bad. Your jokes truly lightened up my days and my PhD time wouldn’t have been as fun without you. I also really appreciate our honest discussions about each other’s data and our motto: garbage in garbage out! During these years you became such a close friend and I really appreciate that. Eleni (a.k.a fantastic labmate or professor

Greece), since the moment you entered the lab I felt we connected well which grew to a close friendship that I really appreciate. You humor contributed a lot to the nice working environment. Working together in the cell culture room made the, sometimes repetitive, work much more fun.

Jamil, I cannot remember how many nicknames I have for you. To name a few: Jamelt, Jamelto,

Jameltinio, Jamolt, Jamolta, professor bullshit, professor Lebanon and one that I don’t dare to write here. I very much enjoyed working with you. I will always remember your ever expanding and detailed scientific knowledge and your innate capacity to come up with new experiments. You are a very brave and strong person encountering unusual problems with regards to your home country. I hope we will share the Dutch nationality sometime soon. Simone, thanks a lot for your support during the master

internship and my PhD. I remember the days when we were the only people working in the lab listening to music in the cell culture room. We had so much fun together! Marta, buenas tardes and its almost

Friday!! Your smile and jokes always filled the lab with happiness which contributed a lot to a nice working environment. Jas, I really enjoyed working and writing with you and this sentence will always

stick in my mind when I don’t want to do a task: ahhg, I can’t be bothered… Sebastian, howdy!

Unfortunately we didn’t spend longer together in the lab but your passion for science and knowledge about many different topics impressed me from the beginning. I am sure you will have a bright future ahead and hope we can drink a beer together soon.

Nynke, your positive vibes, smiling face and willingness to help people are amazing! I always enjoyed

our small talk and discussions about the progression of our projects. I am sure you have a very bright

145

future with lots of fun, traveling and dancing ahead. Ale, I really appreciate your honest and

well-thought opinions about different aspects of life! I learned a lot from you and enjoyed working with you. You will certainly achieve whatever you want. Abel, your positive attitude and funny jokes made

me smile. Your quick way of thinking and connecting processes amazed me from the first moment I met you. Willem, you are probably one of the few people understanding the fun of renovation and

living in the countryside. Your calmness and motivation to do science inspired me. Lotte, first as a

master student and later as a colleague, I really like working with you. You are a smart and happy person and I am sure you will achieve what you want in the future! Michela, thanks a lot for teaching

me about Italian culture and your hard work during the revision of my oxygen manuscript. My students:

Carlos, Nikita and Sarina. I have seen you growing during your internships and you are all very nice

and smart people. Thank you for working hard and I wish you all the best for the future. I would also like to thank all the people working on the first floor, Cor, Christine, Gertrud, Hidde, Josi, Britt, Amr, Tobias, Ellen, Wytse, Rene, Leen, Ester, Mandy, Anita and all other colleagues from ERIBA. Thank you

for the nice working environment, the help during my projects and the interesting discussions. I really enjoyed working with all of you!

Marjolijn, mijn lieve vrouw, ik kan je niet genoeg bedanken voor je steun en doorzettingsvermogen in

de afgelopen jaren. Jij stond altijd achter mijn keuzes, zonder jou was dit nooit gelukt. Jouw rust en vertrouwen hebben me geholpen om door te zetten en me zelfvertrouwen te geven, zelfs toen ik het echt even niet meer zag zitten. Alles deed je zonder ook maar een keer te mopperen als ik weer op onmogelijke tijden naar het lab moest, te moe was of geen tijd had om aan ons huis te klussen of met mijn gedachten bij mijn experimenten zat. Jij steunde me altijd. Ik ben zo ontzettend trots op jou, op alles wat je doet maar vooral dat je bij mij hoort en dat we samen een team zijn. Ik kan niet wachten tot onze dochter geboren wordt en ik hou van je.

Tenslotte wil ik mijn familie bedanken voor de steun in de afgelopen jaren. Julie hebben ervoor gezorgd dat ik de veilige basis had waar ik op kon bouwen en terugvallen. Mam en Erwin, dank voor jullie hulp

tijdens lastige periodes. Even een bakje koffie, een rustig gesprek en de gezelligheid was wat ik soms echt nodig had om dingen op een rijtje te krijgen. Pap en Sieg, bedankt voor de motivatie om te geloven

in mezelf en de overtuiging dat ik kon worden wat ik wilde worden. Sjoerd en Siska, bedankt dat ik bij

jullie mocht wonen toen ik even geen (t)huis had. De warmte en liefde van jullie gezin en stabiele thuis waar ik in terecht kwam heeft me ontzettend geholpen om mezelf terug te vinden. En natuurlijk mijn broers en zus, Jochem, Daan en Annemieke. Jullie liefde, interesse, slechte grappen en gezelligheid

hebben me altijd geholpen om te relativeren en de moed erin te houden. Everybody, thank you and I hope to see you in the future!

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