University of Groningen
Known and unknown functions of TET dioxygenases: the potential of inducing DNA
modifications in Epigenetic Editing
Chen, Hui
DOI:
10.33612/diss.168496242
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Publication date: 2021
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Chen, H. (2021). Known and unknown functions of TET dioxygenases: the potential of inducing DNA modifications in Epigenetic Editing. University of Groningen. https://doi.org/10.33612/diss.168496242
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Propositions
1. TET dioxygenases effectively induce DNA demethylation and can be employed in epigenetic editing for gene expression modulation.
2. Epigenetically silenced genes can be therapeutically reactivated by targeted DNA demethylation.
3. The modular CRISPR/Cas9 system is the preferred platform for epigenetic editing as it does not require de novo design of DNA binding fusion proteins.
4. Exploring the function of TET homologues in early organisms can identify enzymes with new catalytic properties for DNA modifications, which can be effective as effector domain in epigenetic editing.
5. The new 5gmC DNA base modification is involved in gene expression regulation and its writer CrTET1 should be added to the epigenetic editing toolbox.
6. Isolation of mutants with target genes of unknown phenotypes requires strategies of co-selection with marker genes.
7. Donor DNA integration dependent targeted screening processes can be employed to effectively isolate target gene mutants with low editing efficiencies from cell populations.
8. “Do not, for one repulse, give up the purpose that you resolved to effect.” (William Shakespeare)
9. “Our greatest glory consists not in never falling, but in rising every time we fall.” (Oliver Goldsmith)
