University of Groningen
Known and unknown functions of TET dioxygenases: the potential of inducing DNA modifications in Epigenetic Editing
Chen, Hui
DOI:
10.33612/diss.168496242
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Publication date: 2021
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Citation for published version (APA):
Chen, H. (2021). Known and unknown functions of TET dioxygenases: the potential of inducing DNA modifications in Epigenetic Editing. University of Groningen. https://doi.org/10.33612/diss.168496242
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Known and unknown functions of TET
dioxygenases: the potential of inducing DNA
modifications in Epigenetic Editing
Hui Chen
The studies described in this thesis were financially supported by:
Netherlands Organisation for Scientific Research NWO-VIDI University Medical Center Groningen (Abel Tasman fellowship) The National Key R&D Program of China
The National Science Foundation of China
Shanghai Municipal Science and Technology Project Chinese Academy of Sciences
Printing of this thesis was financially supported by:
University of Groningen
University Medical Center Groningen
Cover design: Hui Chen Lay-out: Hui Chen
Printed by: Glideprinting Drukkerijen - The Netherlands ISBN: (printed version)
ISBN: (digital version)
Copyright © 2021 Hui Chen 陈辉
All rights reserved. No parts of this book may be reproduced or transmitted in any form or by any means without written permission of the author and the publisher holding the copyright of the published articles.
Known and unknown functions of TET
dioxygenases: the potential of inducing DNA
modifications in Epigenetic Editing
Proefschrift
ter verkrijging van de graad van doctor aan de
Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. C. Wijmenga,
en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op
woensdag 16 Juni 2021
om 9. 00 uur
door
Hui Chen
geboren op 14 October 1983
te Hubei, China
Promotores: Prof. dr. M.G. Rots Prof. dr. G.L. Xu Beoordelingscommissie: Prof. dr. A. Jeltsch Prof. dr. T. Plosch Prof. dr. P. Hu
To my family
献给我的家人
Paranimfen: Marloes L. de Groote Pytrick G. Jellema
TABLE OF CONTENTS
CHAPETR 1 General introduction to the thesis
CHAPTER 2 Targeting putative DNA demethylases and known
histone modifying enzymes specifically to endogenous genes
CHAPTER 3 Induced DNA demethylation by targeting
Ten-Eleven Translocation 2 to the human ICAM-1 promoter
Nucleic Acids Research: (2014) 42, 1563-1574
CHAPTER 4 A vitamin-C-derived DNA modification catalyzed by
an algal TET homologue Nature: (2019) 569, 581-585
CHAPTER 5 Microhomology-mediated knock-in of donor DNA
precisely in the genome of Chlamydomonas using Cas9-gRNA ribonucleoproteins
Manuscript under submission
CHAPTER 6 Summary & General discussion and Future perspectives APPENDIX 1 Nederlandse samenvatting
APPENDIX 2 List of publications APPENDIX 3 Acknowledgements APPENDIX 4 中文致谢 9 37 59 89 133 173 205 211 219 213
