University of Groningen
Epigenetic editing
Cano Rodriguez, David
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2017
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Cano Rodriguez, D. (2017). Epigenetic editing: Towards sustained gene expression reprogramming in
diseases. University of Groningen.
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Epigenetic Editing
Towards sustained gene expression
reprogramming in diseases
Cover design: Sebastián Suárez G. Lay out: Sebastián Suárez G. Contact: sebastians95@hotmail.com
UMCG institute PhD thesis ISBN (printed): 978-90-367-9958-4 ISBN (electronic): 978-90-367-9957-7
No parts of this thesis may be reproduced or transmitted in any form or by any means electronic or mechanical, without permission of the author.
The research described in this thesis was financially supported by the EU SNN: The northern netherlands provinces alliance.
Epigenetic Editing
Towards sustained gene expression reprogramming in diseases
PhD Thesis
To obtain the degree of PhD at the
University of Groningen
On the authority of the
Rector Magnificus Prof. E Sterken
And in accordance with
the decision of the College of Deans
This thesis will be defended in public on
Wednesday 12 July 2017 at 9:00 hours
By
David Cano-Rodriguez
Born on March 23 1987
in Cali, Colombia
Supervisor Prof. M.G. Rots Co-Promoter Dr. M.H.J Ruiters Assessment Committee Prof. T. Jurkowski Prof. E. Vellenga Prof. G. Molema
Paranymphs
Alejandro Suarez Rivillas Gabriela Tapia Calle
Chapter 1: General Introduction
Chapter 2: Epigenetic editing: On the verge of
reprogram-ming gene expression at will
Chapter 3: Re-expressing epigenetically silenced genes by
inducing DNA demethylation through targeting of Ten-Ele-ven Translocation 2 to any giTen-Ele-ven genomic locus
Chapter 4: Breaking barriers through chromatin: how can
epigenetic context predict targeting efficiency?
Chapter 5: Targeting two different promoters of
endoge-nous RASSF1 to confirm its dual role in cancer
Chapter 6: TCTN2: a novel tumor marker with oncogenic
properties
Chapter 7: Targeted epigenetic editing of SPDEF reduces
mucus production in lung epithelial cells
Chapter 8: Writing of H3K4Me3 overcomes epigenetic
si-lencing in a sustained but context-dependent manner
Chapter 9: General Discussion and Future
Perspecti-ves
Chapter 10: Summary
Appendices
Nederlandse Samenvatting List of Publications and biography Biography Acknowledgements 12 24 40 54 70 84 98 112 132 142 146 147 148 149
