Interaction of the neuronal multipurpose X11a protein with the
copper chaperone CCS
Duquesne, A.E.
Citation
Duquesne, A. E. (2005, October 17). Interaction of the neuronal multipurpose X11a protein
with the copper chaperone CCS. Retrieved from https://hdl.handle.net/1887/3486
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in theInstitutional Repository of the University of Leiden Downloaded from: https://hdl.handle.net/1887/3486
STELLINGEN
Behorend bij het proefschrift:
Interaction of the neuronal multipurpose X11
protein with the copper chaperone CCS
Aude Duquesne
1. The specificity of PDZ2 for CCSIII is essentially brought about by the C-terminal leucine and its carboxy-group.
(This thesis, Chapter IV)
2. The classification for PDZ-ligand interactions as determined by Walma has the best predictive power, thus far.
(Walma, The second PDZ domain of PTP-BL, Thesis, Radboud Universiteit Nijmegen, 2004 and this thesis, Chapter IV)
3. Even if an automated procedure is available, it still can be useful from a didactical point of view to go through that procedure manually.
(This thesis, Chapter III)
4. Sequence-based homology modeling still does not yield structures that have atomic precision and thus experimental structure determination of proteins remains necessary, even if structural homologues are available.
(Duquesne et al., J. Biomol. NMR, in press and referee’s report)
5. The solution of the McConnell equations for chemical exchange given in several standard NMR textbooks is wrong, providing a typical example of a perpetuating error.
6. The large range of affinities (low nM to high M) of PDZ domains for their ligands probably arises from the different methods used. The low binding constants observed with solution methods, as compared to those with immobilization methods, are more in agreement with the regulatory role of PDZ domains.
(Harris and Lim, J. Cell Sci. (2001), 114, 3219-3231)
7. Technological advance and progress in research go hand in hand.
(This thesis, Chapter V)
8. A PhD project is often the negative of a protein structure: it is well-defined at both ends, and rather random in between.
