University of Groningen
Mimicking heart disease in a dish
Kijlstra, Jan David
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Publication date: 2018
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Kijlstra, J. D. (2018). Mimicking heart disease in a dish: Cardiac disease modelling through functional analysis of human stem cell derived cardiomyocytes. Rijksuniversiteit Groningen.
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MIMICKING HEART DISEASE IN A DISH
Cardiac Disease Modelling through Functional Analysis of
Human Stem Cell Derived Cardiomyocytes
Gedrukte versie: 978-94-034-1314-3 Digitale versie: 978-94-034-1313-6
Copyright 2018, J.D. Kijlstra.
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without prior permission from the author.
Design Cover: Jan David Kijlstra Layout: Legatron Electronic Publishing
Printing: Ipskamp Printing, www.proefschriften.net
This research was financially supported by: Graduate School of Medical Sciences
Financial support by the Dutch Heart Foundation for the publication of this thesis is gratefully acknowledged.
The research described in this thesis was supported by a grant of the Dutch Heart Foundation (2013SB013)
Mimicking Heart Disease in a Dish
Cardiac Disease Modelling through Functional Analysis of
Human Stem Cell Derived Cardiomyocytes
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. E. Sterken en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op woensdag 19 december 2018 om 16.15 uur
door
Jan David Kijlstra
geboren op 20 april 1990 te Groningen
Promotor
Prof. dr. P. van der Meer
Copromotor
Prof. dr. I.J. Domian
Beoordelingscommissie
Prof. dr. E.M.J. Verpoorte Prof. dr. M.P. van den Berg Prof. dr. S.A.J. Chamuleau
Paranimfen
Dr. R.H. Bekendam Dhr. G. Kijlstra
Contents
Chapter 1 Introduction and Aims 9
Chapter 2 Integrated Analysis of Contractile Kinetics, Force Generation, 19
and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes (Stem Cell Reports 2015)
Chapter 3 Single-Cell Functional Analysis of Stem-Cell Derived Cardiomyocytes 49
on Micropatterned Flexible Substrates (Current Protocols in Stem Cell Biology 2017)
Chapter 4 Iron deficiency impairs contractility of human cardiomyocytes through 63
decreased mitochondrial function (European Journal of Heart Failure 2018)
Chapter 5 A model of doxorubicin induced cardiotoxicity in hPSC-CMs 93
(Manuscript in preparation)
Chapter 6 Benchmarking the maturation of human pluripotent stem cell-derived 111
cardiomyocytes: where is the finish line and where are we? (Manuscript in preparation)
Chapter 7 Discussion and Future Perspectives 153
Appendices
Dutch Summary | Nederlandse Samenvatting 165
List of Publications 171
Curriculum Vitae 173
