Regulatory DNA binding peptides as novel tools for plant functional genomics
Lindhout, B.I.
Citation
Lindhout, B. I. (2008, October 1). Regulatory DNA binding peptides as novel tools for plant functional genomics. Retrieved from
https://hdl.handle.net/1887/13123
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the
University of Leiden
Downloaded from: https://hdl.handle.net/1887/13123
Note: To cite this publication please use the final published version (if
applicable).
Regulatory DNA binding peptides as novel tools for plant functional
genomics
Beatrice Ingrid Lindhout
Regulatory DNA binding peptides as novel tools for plant functional
genomics
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van de Rector Magnificus prof. mr. P.F. van der Heijden volgens besluit van het College voor Promoties
te verdedigen op woensdag 1 oktober 2008 klokke 16.15 uur
door
Beatrice Ingrid Lindhout
geboren te Amsterdam in 1977
Promotie commissie
Promotor: Prof. Dr. P.J.J. Hooykaas Co-promotor: Dr. Ir. E.J. van der Zaal
Referent: Dr. H. de Jong (Wageningen Universiteit)
Overige leden: Prof. Dr. H.P. Spaink Prof. Dr. J. Memelink Prof.Dr. P.M. Brakefield
Cover design: F.M. Warmenhoven
Printed by: Ridderprint, Ridderkerk, The Netherlands.
ISBN: 978-90-5335-160-4
Contents
Chapter 1 General Introduction 7
Chapter 2 Effects of differently designed artificial polydactyl zinc finger transcription factors on the regulation of genomic targets in yeast
23
Chapter 3 Live cell Imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteins
53
Chapter 4 Employing libraries of zinc finger artificial transcription factors to screen for homologous recombination mutants in Arabidopsis
75
Chapter 5 VP16-HRU, a zinc finger artificial transcription factor affecting somatic homologous recombination
97
Summary 139
Nederlandse samenvatting 145
Curriculum vitae 150
Publications 151
Colour supplement 152
ABBREVIATIONS
CLSM confocal laser scanning microscope Cy3 cyanine dye 3
Cy5 cyanine dye 5
DNA deoxyribonucleic acid DSB double strand break DSBR double strand break repair
EAR ERF-associated amphiphilic repression motif eGFP enhanced green fluorescent protein EMSA electrophoretic mobility shift assay GFP green fluorescent protein
GUS glucuronidase
HPT hygromycin phosphotransferase HR homologous recombination KRAB kruppel-associated box lacO lac operator
MaSat major satellite
NHR non homologous recombination MMS methyl methanesulfonate NER nucleotide excision repair NLS nuclear localization signal PCR polymerase chain reaction PZF polydactyl zinc finger RFP red fluorescent protein RNA ribonucleic acid RPS5A ribosomal protein S5A SID sin3A interaction domain SSA single strand annealing SSM single molecule microscopy tetO tet operator
VP16 viral protein 16 from herpes simplex virus
Q-RT-PCR quantitative reverse transcription polymerase chain reaction
ZF zinc finger
ZF-ATF zinc finger artificial transcription factor
