University of Groningen
Development of MAPC derived induced endodermal progenitors Sambathkumar, Rangarajan
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Publication date: 2017
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Sambathkumar, R. (2017). Development of MAPC derived induced endodermal progenitors: Generation of pancreatic beta cells and hepatocytes. University of Groningen.
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Propositions
Associated with the thesis
Development of MAPC derived induced endodermal progenitors
Generation of pancreatic beta cells and hepatocytes
1. New sources for renewable stem or progenitor cells will make cell therapy for diabetes and liver diseases on a large scale a feasible option.
2. It takes 14 transcription factors (TFs) and specific culture conditions to reprogram human Multipotent Adult Progenitors into Induced Endodermal Progenitor cells (iENDO) (this thesis).
3. iENDO cells can be expanded for a long time in culture without loosing endodermal characteristics (this thesis).
4. Undifferentiated iENDO cells can spontaneously differentiate into hepatocyte and endocrine pancreatic like cells, as wells as gut-‐tube like cells (this thesis).
5. In vitro, undifferentiated iENDO cells can differentiate into hepatocyte and endocrine pancreatic-‐like cells using 3D organoid culture methods but this does not stimulate maturation (this thesis).
6. iENDO-‐derived hepatocyte like cells transplanted in mice can mature in cells hepatocyte characteristics and do not form tumors (this thesis).
7. Trichostatin-‐A treatment with chromatin remodeling medium can lead to formation of definitive and pancreatic endoderm cells differentiation in fibroblasts (this thesis).
8. Although complicated to realize, stem cell based therapy is still the best hope for modern medicine and will lead to new treatments of diseases in the near future.
9. A PhD training in Europe is a great learning period not only for research but also for life. It provides great wisdom and it teaches how to be independent and organized in life and prepared for survival in the foreign land.
10. As a foreigner, Europe provides the correct balance between friendliness, comfort and technology to perform research at a high level.
11. Failure is the first key step to success and to realize your dreams.
