Image processing and computing in structural biology
Jiang, L.
Citation
Jiang, L. (2009, November 12). Image processing and computing in structural biology. Retrieved from https://hdl.handle.net/1887/14335
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/14335
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Image Processing and Computing in Structural Biology
PROEFSCHRIFT
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. P. F. van der Heijden,
volgens besluit van het College voor Promoties, te verdedigen op donderdag 12 November 2009
klokke 15.00 uur
door
Linhua Jiang
Geboren te Yongzhou, China in 1977
Promotiecommissie
Promotor: Prof. dr. J.P. Abrahams
Overige leden: Prof. dr. H.W. Zandbergen (TUD, Delft)
Prof. dr. M. van Heel (Imperial College, London) Prof. dr. M.H.M. Noteborn
Prof. dr. N. Ban (ETH, Zurich) Dr. F.J. Verbeek
Dr. J.R. Plaisier (ELETTRA, Trieste) Dr. M.E. Kuil
Dr. R.A.G. de Graaff
Cover: The ribosomal large subunit 50S and cryo-electron microscopy
ISBN: 978-90-8570-293-1
Copyright © by Linhua Jiang 2009
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without the prior written permission of the copyright owner.
Printed by Wöhrmann Print Service, The Netherlands.
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Contents
Chapter 1 Introduction 5
Chapter 2 Automated carbon masking and particle picking in data preparation of single particles 21
Chapter 3 A novel approximation method of CTF amplitude correction for 3D single particle reconstruction 41
Chapter 4 Reconstruction of the complexes of the ribosomal large subunit 50S with Hsp15 and t-RNA reveals the rescue mechanism of the stalled 50S 67
Chapter 5 Unit-cell determination from randomly oriented electron diffraction patterns 91
Chapter 6 User manual of EDiff: A unit-cell determination and indexing software 109
Chapter 7 Conclusion and Perspectives 139
Summary
141
Samenvatting
144
Curriculum Vitae 146
A Special Word of Thanks 147
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