Cover Page
The handle
http://hdl.handle.net/1887/77743
holds various files of this Leiden University
dissertation.
Author: Strunks, G.D.
Strategies for
the improvement
of genome editing in
Arabidopsis thaliana
Gary Strunks
Strategies for the improvement of genome editing in Arabidopsis thaliana
PhD thesis, Leiden University, 2019
Copyright © Gary Strunks, 2019. All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, without the prior written permission of the copyright holder.
ISBN | 978-94-6375-495-8
Strategies for the improvement of genome editing in
Arabidopsis thaliana
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op woensdag 11 september 2019
klokke 11.15 uur
door
Gary Daniël Strunks geboren te Amsterdam, Nederland
Promotor: prof.dr. P.J.J. Hooykaas
Copromotor: dr. B.S. de Pater
Promotiecommissie: prof.dr. G.P. van Wezel (voorzitter)
prof.dr. R. Offringa (secretaris)
Overige commissieleden: prof.dr. Y. Bai, Wageningen UR
dr. F.A. Krens, Wageningen UR
Chapter 1 General introduction 9
Chapter 2 Sequence-specific nucleases for genome editing in plants: 47
TALENs versus CRISPR/Cas9
Chapter 3 CRISPR/Cas9-induced double strand break repair in Arabidopsis 63
nonhomologous end-joining mutants
Chapter 4 In planta gene targeting during meiosis 89
Chapter 5 Developing a strategy for the identification of gene targeting mutants 121
in Arabidopsis thaliana
Chapter 6 Summarizing discussion 149
Chapter 7 Samenvatting 157
Curriculum Vitae 165
