University of Groningen
Exploring the metabolic potential of Penicillium rubens
Viggiano, Annarita
DOI:
10.33612/diss.126598491
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Publication date:
2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Viggiano, A. (2020). Exploring the metabolic potential of Penicillium rubens. University of Groningen.
https://doi.org/10.33612/diss.126598491
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STELLINGEN
Exploring the metabolic potential of Penicillium rubens
Annarita Viggiano
1. Microorganisms are a rich source of secondary metabolites, many of which have interesting properties for human applica-tions ( chapter 1). 2. The advances in genetic engineering have deepened our know-ledge of secondary metabolism in filamentous fungi and will lead to the discovery, characterization and production of bioactive molecules (chapter 1). 3. The chrysogine biosynthetic pathway in P. rubens is highly bran-ched and consists of at least 14 compounds (chapter 2). 4. A combination of genetic engineering approaches is essential for elucidating complex biosynthetic pathways (chapter 2). 5. Not always gene overexpression results in sufficient and stable transcript levels or production of novel molecules (chapter 3). 6. P. rubens proved to be a versatile host for the expression of genes and production of metabolites from other fungi, with a choice for various heterologous fungal promoters and markers. However, the expression of bacterial genes has only partially be explored and requires further investigations and optimization, in order to use P. rubens as a platform for the screening of silent biosynthetic genes, the elucidation of uncharacterized pathways and the pro-duction of novel metabolites from bacteria (chapter 4). 7. We must have perseverance and above all confidence in ourselves (Marie Curie). 8. Whatever decision you have made for your future, you are au-thorized and encouraged to review it, ready to change it if it no longer meets your wishes (Rita Levi Montalcini).
