University of Groningen
Biochemical characterization and bioinformatic analysis of two large multi-domain enzymes
from Microbacterium aurum B8.A involved in native starch degradation
Valk, Vincent
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Publication date: 2017
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Valk, V. (2017). Biochemical characterization and bioinformatic analysis of two large multi-domain enzymes from Microbacterium aurum B8.A involved in native starch degradation. Rijksuniversiteit Groningen.
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Biochemical characterization and
bioinformatic analysis of two large
multi-domain enzymes from
Microbacterium aurum B8.A
involved in native starch
degradation
Cover design: RivVin
Printed by: Ipskamp Printing, Enschede ISBN printed: 978-90-367-9498-5
ISBN digital: 978-90-367-9497-8
The studies presented in this thesis were performed within the framework of the Top Institute Food and Nutrition (TIFN).
The work described in this thesis was carried out in the Microbial Physiology Research group which is part of the Groningen Biomolecular Sciences and Biotechnology institute (GBB) of the Universtity of Groningen, the Netherlands. This research was funded by the Top Institute Food & Nutrition (project B1003) and the University of Groningen.
Biochemical characterization and
bioinformatic analysis of two large
multi-domain enzymes from
Microbacterium aurum B8.A
involved in native starch
degradation
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. E. Sterken en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op vrijdag 27 januari 2017 om 12:45 uur
door
Vincent Valk
geboren op 24 juli 1982 te Hardenberg
Promotor
Prof. dr. L. Dijkhuizen
Copromotor
Dr. ir. R.M. van der Kaaij
Beoordelingscommissie
Prof. dr. B. Svensson Prof. dr. D.B. Janssen Prof. dr. K.U. Loos
Chapter 1 Introduction Chapter 2
Degradation of granular starch by the bacterium Microbacterium aurum B8.A involves a novel modlar α-amylase enzyme system with FNIII and CBM25 domains
Chapter 3
Carbohydrate Binding Module 74 is a novel starch binding domain associated with large and multi-domain α-amylase enzymes Chapter 4
Characterization of the starch-acting MaAmyB enzyme from
Microbacterium aurum B8.A representing the novel subfamily GH13_42 with an unusual, multi-domain organization
Chapter 5
The evolutionary origin and possible functional roles of FNIII domains in two Microbacterium aurum B8.A granular starch degrading enzymes, and in other carbohydrate acting enzymes
Chapter 6
Summary and Discussion References
Nederlandse samenvatting
Samenvatting voor een breed publiek Dankwoord Publicaties 9 29 51 73 103 121 133 147 161 167
Contents
Introduction