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Peripheral nerve graft architecture affects regeneration Vleggeert-Lankamp, Carmen Lia Anne-Marie

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(1)Peripheral nerve graft architecture affects regeneration Vleggeert-Lankamp, Carmen Lia Anne-Marie. Citation Vleggeert-Lankamp, C. L. A. -M. (2006, December 14). Peripheral nerve graft architecture affects regeneration. Retrieved from https://hdl.handle.net/1887/5566 Version:. Corrected Publisher’s Version. License:. Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden. Downloaded from:. https://hdl.handle.net/1887/5566. Note: To cite this publication please use the final published version (if applicable)..

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(16) 'JHVSF-JHIUNJDSPTDPQJDNJEHSBGUDSPTTTFDUJPOTPGSFHFOFSBUJOHOFSWFT. A:Nonpor ousgraft. B:Por ousgraft. 0.5 mm. 50 mm. . 50 mm. 5SBOTWFSTF ǃN DSPTTTFDUJPOTPGNJEHSBGUTDJBUJDOFSWFT JNNFSTJPOmYFEBGUFSFMFDUSPQIZTJPMPHJDBMFWBMVBUJPO BOETUBJOFE XJUIIBFNBUPYZMJOFPTJO BDDPSEJOHUPUIFNFUIPEPG,MàWFS#BSSFSBUPDMFBSMZJEFOUJGZNZFMJOTIFBUIT<> BOEBDDPSEJOH UPUIFNFUIPEPG7FSIPFGWBO(JFTPOUPDMFBSMZJEFOUJGZDPMMBHFO<>BSFEFQJDUFE*OBMMmHVSFT UIFMFGUQBOFMSFQSFTFOUT UIF7FSIPFGWBO(JFTPOTUBJOFEOFSWFTFDUJPOBTBXIPMF&MBTUJDmCSFTBSFTUBJOFECMBDL OVDMFJCMVFBOEDZUPQMBTNZFMMPX CSPXO5IFNJEEMFQBOFMSFQSFTFOUTBOFOMBSHFETBNQMFPGUIFIBFNBUPYZMJOFPTJOOFSWFTFDUJPOBOEEFNPOTUSBUFTUIF NPSQIPMPHZPGUIFOFSWFJOEFUBJM5IFSJHIUQBOFMEFQJDUTBOFOMBSHFETBNQMFPGUIFOFSWFTFDUJPO TUBJOFEBDDPSEJOHUPUIF NFUIPEPG,Mà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mH MFGUDPMVNO 5IFNZFMJO TIFBUITPGUIFOFSWFmCSFTJOHSBGUFEOFSWFTXFSFPCWJPVTMZUIJOJODPNQBSJTPOUPUIPTFJO DPOUSPMT5IFOFSWFmCSFTJOBVUPHSBGUQFSPOFBMOFSWFT BOETQPSBEJDBMMZJOTZOUIFUJDOFSWF HSBGUFEOFSWFT XFSFGSFRVFOUMZCVOEMFEXJUIJOBSFBTPGTJ[FDPNQBSBCMFUPUIFDSPTTTFDUJPO PGTJOHMFNZFMJOBUFEmCSFTJODPOUSPMOFSWFT TVHHFTUJOHSFHFOFSBUJPOPGTFWFSBMOFSWFmCSFT UISPVHITJOHMF4DIXBOODFMMCBTBMMBNJOBSTDBõPMETUIBUQFSTJTUFEBGUFS8BMMFSJBOEFHFOFSB UJPOPGUIFPSJHJOBMNZFMJOBUFEOFSWFmCSFT<>5IFmCSFEJBNFUFSGSFRVFODZEJTUSJCVUJPOT mH NJEEMFBOESJHIUDPMVNO EFNPOTUSBUFEUIBUJOSFHFOFSBUJOHQFSPOFBMOFSWFTmCSF EJBNFUFSTTIJGUFEUPTNBMMFSTJ[FT. . .

(17) 'JHVSF-JHIUNJDSPTDPQJDDSPTTTFDUJPOTBOEGSFRVFODZEJTUSJCVUJPOTPGDPOUSPMBOEHSBGUQFSPOFBMOFSWFT. A:Contr ol 250 6. F[f] (%). n[f]. 200. 150. 4. 100 2. 50. 0. 0. 1. 3. 5. 7. 9. 11 13. 0. 2. 4. 6. d[f] (mm). 8. 10. 12. 14. 16. 18. 12. 14. 16. 18. 12. 14. 16. 18. 12. 14. 16. 18. d[f] (mm). B: Autograft 250. 6. . F[f] (%). 200. 150. n[f]. . 4. 100. 2 50. 0. 0. 1. 3. 5. 7. 9. 11 13. 0. 2. 4. 6. d[f] (mm). 8. 10. d[f] (mm). C:Nonpor ous 250. 6. F[f] (%). 200. n[f]. 150. 4. 100. 2 50. 0. 0 1. 3. 5. 7. 9. 11 13. 0. 2. 4. 6. d[f] (mm). D:Por ous. 8. 10. d[f] (mm). 20 mm 250. 6. F[f] (%). n[f]. 200. 150. 4. 100. 2 50. 0. 0 1. 3. 5. 7. 9. d[f] (mm). 11 13. 0. 2. 4. 6. 8. 10. d[f] (mm). 5SBOTWFSTFTFNJUIJO ǃN DSPTTTFDUJPOTPGQFSPOFBMOFSWFT JNNFSTJPOmYFEBGUFSFMFDUSPQIZTJPMPHJDBMFWBMVBUJPO BOE TUBJOFEXJUIBUPMVJEJOFCMVFCPSBYTPMVUJPO<>BSFEFQJDUFE*OBMMmHVSFT UIFMFGUQBOFMSFQSFTFOUTUIFOFSWF TFDUJPOBTBXIPMF XIFSFBTJOUIFNJEEMFQBOFMBOFOMBSHFETBNQMFPGUIJTOFSWFTFDUJPO TRVBSF EFNPOTUSBUFTUIF NPSQIPMPHZPGUIFOFSWFJOEFUBJM5IFSJHIUQBOFMEFQJDUTUZQJDBMFYBNQMFTPGUIFGSFRVFODZEJTUSJCVUJPOTPGmCSFEJBNFUFST PGDPOUSPM BVUPHSBGUFE OPOQPSPVT BOEQPSPVTHSBGUFEQFSPOFBMOFSWFT*OFBDIHSBQI EJBNFUFSTPGUIFOFSWFmCSFTXFSF EJTUSJCVUFEJOUPDMBTTFTPGǃNFBDI BOEQMPUUFEBHBJOTUUIFmCSFEJBNFUFSDMBTTGSFRVFODZ5IFEPUTSFQSFTFOUUIF DBMDVMBUFEmCSFEJBNFUFSDMBTTGSFRVFODZQFSmCSFEJBNFUFSDMBTT UIFTUSBJHIUMJOFSFQSFTFOUTUIFmUPGUPUBMDVSWF UIF EPUUFEMJOFSFQSFTFOUTUIF"ƹmCSFQPQVMBUJPO BOEUIFEBTIFEMJOFSFQSFTFOUTUIF"ƸmCSFQPQVMBUJPO.

(18) 'JHVSF.PSQIPNFUSJDBOBMZTJT. A. B. D. C. 3. *. #. 2. # 10. Porous. Nonporous. Control. Nonporous. Porous. Control. 0. Autograft. Porous. Autograft. Nonporous. 20. Autograft. *. 4. 0. Control. Porous. Nonporous. Control. Autograft. E. F. . *. 4. . 8. # 2. #. 0. *. *. 4. * *. * *. Porous. Nonporous. Autograft. Porous. Nonporous. Autograft. Control. 0. Control. 3. *. 4. 0. 0. Number of nerve fibres (x 10 ). Number of fibres (x10 ). Nerve diameter (mm). 100. *. Nerve diameter (mm). Nerve area (x103 mm2). 8 200. Fibre density (x103/mm2). *. .PSQIPNFUSJDEBUBPGUIFDPOUSPM BVUPHSBGUFEBOETZOUIFUJDHSBGUFEOFSWFTBSFEFQJDUFE"5IFUPUBMTVSGBDFPGUIFOFSWF DSPTTTFDUJPO OFSWFBSFB #UIFOFSWFmCSFEJBNFUFS $UIFOVNCFSPGOFSWFmCSFT %UIFOFSWFmCSFEFOTJUZ &UIFOVNCFS PGOFSWFmCSFTGPSUIF"ƸBOE"ƹmCSFQPQVMBUJPOTFQBSBUFMZ BOE'UIFOFSWFmCSFEJBNFUFSGPSUIF"ƸBOE"ƹmCSF QPQVMBUJPOTFQBSBUFMZ%BUBBSFSFQSFTFOUFEBTNFBO œTE  %JõFSFOUDPNQBSFEUPDPOUSPMOFSWFT  %JõFSFOUDPNQBSFE UPBVUPHSBGUOFSWFT. "MMOFSWFBSFBTXFSFPGDPNQBSBCMFTJ[F mH" BOEUIFmCSFEJBNFUFSTPGHSBGUFEOFSWFT XFSFIBMWFEDPNQBSFEUPDPOUSPMT mH# 5IFUPUBMOVNCFSBOEUIFEFOTJUZPGOFSWFmCSFT XFSFEPVCMFEBGUFSBVUPHSBGUJOH BOESFNBJOFEVODIBOHFEJOTZOUIFUIJDHSBGUFEOFSWFT mH $BOE% *O QPSPVT CVUOPUJO OPOQPSPVT OFSWFHSBGUTUIFOVNCFSPGOFSWFmCSFTBOEUIF OFSWFmCSFEFOTJUZXBTTNBMMFSDPNQBSFEUPBVUPHSBGUT ,PMNPHPSPW4NJSOPXUFTUTSFWFBMFEBIPNPHFOFPVTmCSFTJ[FEJTUSJCVUJPOUISPVHIPVU DPOUSPMOFSWFT BOEBIPNPHFOFPVTmCSFTJ[FEJTUSJCVUJPOXJUIJOJOEJWJEVBMDPOUSPMOFSWFT *OUIFSFHFOFSBUJOHOFSWFT CPUIIFUFSPHFOFJUZBOEIPNPHFOFJUZJOmCSFTJ[FEJTUSJCVUJPOT CFUXFFOOFSWFT BOEXJUIJOJOEJWJEVBMOFSWFT XFSFFODPVOUFSFE 5IFTVNPGUXPMPHOPSNBMEJTUSJCVUJPOTDPVMETBUJTGBDUPSJMZCFmUUFEUPUIFmCSFEJBNFUFS EJTUSJCVUJPOTPGCPUIDPOUSPMBOEHSBGUQFSPOFBMOFSWFT mH SJHIUDPMVNO *OBMMFYBNJOFE OFSWFT UIF"*$XBTEFUFSNJOFEBOETIPXOUPCFTNBMMFS Q. . GPSUXPQPQVMBUJPOTDPN . QBSFEUPPOF JOEJDBUJOHUIBUUIFmCSFEJBNFUFSEJTUSJCVUJPODPVMECFNPSFBQUMZEFTDSJCFECZ UIFTVNPGUXPMPHOPSNBMEJTUSJCVUJPOTSBUIFSUIBOCZPOFMPHOPSNBMEJTUSJCVUJPO.

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(21) 'JHVSF4UJNVMVTSFDSVJUNFOUDVSWFT. 40. 40. 30. 30. Q (pC). Q (pC). A:Q/V graphs. Aa. 20. Aa. 20. Ab 10. 10. Ab. 0. 0. 0. . 1. 2. 3. 4. 5. 7. 6. 9. 8. 0. 1. 2. 3. 4. 5. Voltage (V). Voltage (V). Nonporous. Porous. 9. 8. D: V50,a/b. C: V50. B:Q max,StR. 7. 6. . *. 5. 5 800. V50 (V). 3. *. ^. *. Porous. 0. Nonporous. 1. 0. Autograft. 2. 1. Control. Porous. Nonporous. *. ^. Control. ^ * # * #. Autograft. ^ 0. Autograft. * #. 2. * * # #. Control. 3. Porous. ^ ^. 4. Nonporous. V50 (V). Charge (pC). *. 400. *. *. 4. "4UJNVMVTSFDSVJUNFOUHSBQITDPSSFTQPOEJOHUPUIFFYBNQMFTEFQJDUFEJOmHVSF"4PMJEDJSDMFTSFQSFTFOUEBUBQPJOUT $POUJOVPVTMJOFTSFQSFTFOUUIFmUUFEDVSWFT5IF"*$QSPWFEUPCFTNBMMFS Q  GPSUXPQPQVMBUJPOTDPNQBSFEUPPOF #5IF2 NBY Ƹ EBSLHSFZ BOE2 NBY ƹ MJHIUHSFZ PGDPOUSPM BVUPHSBGUFE  OPOQPSPVTBOE QPSPVTHSBGUFEOFSWFTEFSJWFEGSPN UIFTUJNVMVTSFDSVJUNFOUEBUB5IFQSFEJDUFEWBMVFTPG2 NBY ƸBOE2 NBY ƹBSFSFQSFTFOUFECZVOmMMFECBST$.FBOWPMUBHF UISFTIPME 7  PGDPOUSPM BVUPHSBGUFE BOETZOUIFUJDHSBGUFETDJBUJDOFSWFT%.FBOmSJOHUISFTIPMEGPSUIF"ƸBOE"ƹ mCSFTTFQBSBUFMZ 7  ƸBOE7  ƹ PGDPOUSPM BVUPHSBGUFE BOETZOUIFUJDHSBGUFETDJBUJDOFSWFT5IFQSFEJDUFEWBMVFTPG7  Ƹ BOE7  ƹBSFSFQSFTFOUFECZVOmMMFECBST%BUBBSFSFQSFTFOUFEBTNFBO œTE  %JõFSFOUDPNQBSFEUPDPOUSPMHSPVQ  %JõFSFOUDPNQBSFEUPBVUPHSBGUHSPVQ ~ %JõFSFOUDPNQBSFEUPQSFEJDUFEWBMVFT Q ~~ %JõFSFOUDPNQBSFEUP QSFEJDUFEWBMVFT Q. UISFTIPMEWPMUBHFPGUIF"ƸmCSFT 7.  Ƹ. XBTBQQSPYJNBUFMZIBMGUIFWBMVFPG7. BTJOUIF.  ƹ. DPOUSPMOFSWFT 5IFJOUFSQVMTFUJNFSFDSVJUNFOUDVSWFT DPSSFTQPOEJOHUPUIPTFOFSWFTPGXIJDIUIFTUJNV  MVTSFDSVJUNFOUDVSWFTXFSFEFQJDUFEJOmHVSF" EFNPOTUSBUFEBHBJOUIBUUIFDPOUSJCVUJPO PGUIF"ƸmCSFTUP2 NBYXBTMBSHFSUIBOUIFDPOUSJCVUJPOPGUIF"ƹmCSFT mH" BTJODPOUSPM BOEBVUPHSBGUFEOFSWFT mH# -JLFXJTF CFUXFFOTZOUIFUJDOFSWFHSBGUTOPEJõFSFODFTJO 2NBY ƸPS2 NBY ƹXFSFFODPVOUFSFE mH#  5IFSFTQPOTFJOUIFHSBGUFEOFSWFTXBTDIBSBDUFSJ[FECZBMPOHFSNFBOSFGSBDUPSZQFSJPE U  DPNQBSFEUPDPOUSPMOFSWFT mH$ 5IFSFGSBDUPSZQFSJPEEPVCMFEJOBVUPHSBGUFEBOE QPSPVT HSBGUFEOFSWFT CVURVBESVQMFEJO OPOQPSPVTHSBGUFEOFSWFT5IFWBMVFPGU  ƸEFNPOTUSBUFEB TJNJMBSQBUUFSO mH% 5IFWBMVFPGU.  ƹ. BQQSPYJNBUFMZEPVCMFEJOUIFBVUPHSBGUFE USJQMFEJO.

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