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Additional file 2.

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Tecbh1-TrCBM-C 420 VPSDVESQSPNSYVTYSNIKFGPINSTFT---ASNP---PGGN 461 Tecbh1-CtCBM-C 420 VPSDVESQSPNSYVTYSNIKFGPIGSTVPGLDGSNPGNPTTTVVPPASTSTS 476 Tecbh1-HgCBM-C 420 VPSDVESQSPNSYVTYSNIKFGPIGSTVAGLPGAGNGGNN---GGNPP 469 ************************.**.. .:. . Tecbh1-TrCBM-C RGTTTTRRPATT-TGSSPGPTQSHYGQCGGIGYSGPTVCASGTTCQVLNPYYSQCL 516 Tecbh1-CtCBM-C RPTSSTSSPVSTPTGQPGGCTTQKWGQCGGIGYTGCTNCVAGTTCTQLNPWYSQCL 532 TeCBH1-HgCBM-C PPTTTTSSAPATTTTASAGPKAGRWQQCGGIGFTGPTQCEEPYICTKLNDWYSQCL 525 *::* . :* * . * . :: ******::* * * * ** :*****

Additional file 2. Partial amino acid sequence alignment of the C-terminal end of T.e.CBH1

fused to the T. reesei, C. thermophilum or H. grisea linker-CBM sequences. The last amino acid

of the native T.e.CBH1, Ser455, is shown in bold type in the TeCBH1-CBM-C sequence. The

cysteine residues in the CBM that take part in disulfide bridge formation are shown in bold

type and the aromatic amino acids predicted to bind cellulose are underlined. The

T.e.CBH1-T.r.CBM-C enzyme has an additional N-glycosylation target site (Asn449) lacking from the

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