University of Groningen
Identification and characterization of flavoprotein monooxygenases for biocatalysis
Gran Scheuch, Alejandro
DOI:
10.33612/diss.154338097
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Publication date: 2021
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Gran Scheuch, A. (2021). Identification and characterization of flavoprotein monooxygenases for biocatalysis. University of Groningen. https://doi.org/10.33612/diss.154338097
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Identification and characterization
of flavoprotein monooxygenases
for biocatalysis
The research described in this thesis was performed at:
• Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of the Groningen, The Netherlands.
• Department of Chemical and Bioprocesses Engineering, School of Engineering, Pontificia Universidad Católica de Chile, Santiago, Chile.
The research conducted in this thesis was financially supported by:
This work was supported by the Institute for Biological and Medical Engineering from Pontificia Universidad Católica de Chile and CORFO’s grant 14ENI2-26862. A Ph.D. scholarship from ANID (Agencia Nacional de Investigacion y Desarrollo, ex-CONICYT; scholarship 21151392), from VRI of Pontificia Universidad Católica de Chile, from the Faculty of engineering of Pontificia Universidad Católica de Chile and from the University of Groningen are acknowledged.
Printing of this thesis was financially supported by:
University of Groningen
Cover design
Alejandro A. Gran Scheuch
Layout
Douwe Oppewal, www.oppewal.nl
Identification and characterization
of flavoprotein monooxygenases
for biocatalysis
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga
and in accordance with
the decision by the College of Deans
and
to obtain the degree of PhD at the
Pontificia Universidad Católica de Chile
on the authority of the
Rector Prof. I. Sánchez Diaz
and in accordance with
the decision by the the Office of Graduate Studies
Double PhD degree
This thesis will be defended in public on
Friday 8 January 2021 at 14.30 hours
by
Alejandro Alberto Gran Scheuch
born on 26 December 1989
Supervisors:
Prof. Dr. M.W. Fraaije
Prof. Dr. D.B. Janssen
Co-supervisor:
Dr. L. Parra
Assessment Committee:
Prof. Dr. J.A. Asenjo
Prof. Dr. M. Vásquez
Prof. Dr. W.J.H. van Berkel
Prof. Dr. A.J.M. Driessen
TABLE OF CONTENTS
Chapter I Baeyer-Villiger monooxygenases, tunable oxidative biocatalysts 7
Chapter II Optimizing the linker length for fusing an alcohol dehydrogenase 57 with a cyclohexanone monooxygenase
Chapter III Systematic assessment of uncoupling in flavoprotein oxidases and 89 monooxygenases
Chapter IV Genome mining of oxidation modules in trans–acyltransferase 123 polyketide synthases reveals a culturable source for lobatamides
Chapter V Identification of two flavin-containing monooxygenases from 161 Chloroflexi and Hypsibius dujardini
Chapter VI Mining the genome of Streptomyces leeuwenhoekii: 187 Two new type I Baeyer-Villiger monooxygenases
from Atacama Desert
Chapter VII Summary and conclusions 223
Appendices Nederlandse samenvatting 231
Resumen en español 237
List of publications 242
