University of Groningen
Characterization and computation-supported engineering of an ω-transaminase Meng, Qinglong
DOI:
10.33612/diss.172243517
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Publication date: 2021
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Citation for published version (APA):
Meng, Q. (2021). Characterization and computation-supported engineering of an ω-transaminase. University of Groningen. https://doi.org/10.33612/diss.172243517
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1
Characterization and
computation-supported engineering of an
ω-transaminase
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga and in accordance with
the decision by the College of Deans. This thesis will be defended in public on
Tuesday 29 June 2021 at 14.30 hours
by
Qinglong Meng
born on 8 June 1988 in Tianjin, China
2
Supervisors
Prof. D.B. Janssen Prof. M.W. FraaijeAssessment committee
Prof. G.J. Poelarends Prof. G. Maglia Prof. J. Pleiss3
TABLE OF CONTENTS
Chapter I General introduction 4
Part 1 Protein engineering of amine transaminases 5
Part 2 Aims of the thesis 33
Chapter Ⅱ Substrate spectrum of an ω-transaminase from Pseudomonas jessenii 39
analyzed experimentally and by docking simulations Chapter Ⅲ Robust ω-transaminases by computational stabilization of the subunit 55
interface Chapter Ⅳ Asymmetric synthesis of optically pure aliphatic amines with an 95
engineered robust ω-transaminase Chapter Ⅴ Computational redesign of an ω-transaminase from Pseudomonas 121
jessenii for asymmetric synthesis of enantiopure bulky amines Summary and perspectives 157
Nederlandse samenvatting 165
List of publications 174
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