University of Groningen Let op! Cell wall under construction Morales Angeles, Danae

University of Groningen

Let op! Cell wall under construction

Morales Angeles, Danae

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Publication date: 2018

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Morales Angeles, D. (2018). Let op! Cell wall under construction: Untangling Bacillus subtilis cell wall synthesis. University of Groningen.

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Stellingen  

Behorende  bij  het  proefschrift  

LET  OP!  CELL  WALL  UNDERCONSTRUCTION

Untangling  Bacillus  subtilis  cell  wall  synthesis  

Danae  Morales  Angeles

1.   The  synthesis  of  the  cell  wall  involves  many  reactions  and  enzymes,  but  these  can  be   different   between   bacterial   species   or   growth   conditions.   In   summary,   cell   wall   synthesis  is  a  complex  process  to  study  (Chapter  1).  

2.   PP-­‐nisin  and  ΔΔ-­‐nisin  are  able  to  form  pores  in  live  cells,  although  previous  reports   showed  both  derivatives  cannot  form  pores  in  giant  unilamellar  vesicles  (Chapter  

2).  

3.   The  Bacillus  subtilis  division  site  is  enriched  in  peptidoglycan  in  which  the  last  D-­‐Ala   of  the  pentapeptide  has  not  been  cleaved  (Chapter  3).  

4.   PBP2B  is  the  only  essential  PBP  of  Bacillus  subtilis.  Remarkably,  inactivation  of  the   active  site  of  PBP2B  does  not  have  an  effect  on  Bacillus  viability  (Chapter  3).  

5.   Many  functions  have  been  attributed  to  the  so-­‐called  PASTA  domains.  But  until  now,   there  is  no  consensus  on  the  functions  they  can  actually  execute  (Chapter  4).  

6.   The   length   defect   and   sensitivity   to   heat   that   the   cells   present   when   the   PASTA   domains  of  PBP2B  are  deleted,  reflect  the  importance  of  the  domains  (Chapter  4).      

7.   A  microscopy  picture  does  not  reflect  all  the  work  that  is  behind  it.      

8.   Figuring   out   what   bacteria   have   developed   for   their   evolution   and   survival   is   the   quest.   It’s   you   against   the   bacterium.   That’s   the   competition…   Can   you   trick   this   bacterium  into  giving  up  its  secrets?  Guess  who’s  not  winning?  –  Bonnie  L.  Bassler    

9.   Motivation   gets   you   going,   but   discipline   keeps   you   growing.   That’s   the   Law   of   Consistency.   It   doesn’t   matter   how   talented   you   are.   It   doesn’t   matter   how   many   opportunities  you  receive.  If  you  want  to  grow,  consistency  is  key.  -­‐  John  C.  Maxwell    

10.   Everything  tends  towards  entropy  –  maybe  the  best  examples  of  this  were  my  desk   and  lab  bench  during  my  PhD.  Second  law  of  thermodynamics