GGS
Basic Principles of Gene and Protein Technology (G. Volckaert) Contents:
- Enzymatic armamentorium of recombinant DNA: fragmentation approaches & other applications - Immobilizing nucleic acids and proteins: hybridisation and screening methods
- Cloning vectors: general cloning vectors, multipurpose and special-purpose vectors - Basal techniques of DNA cloning: from genes to clones and from clones to genes - Genome and cDNA libraries, and their exploration
- Expression strategies, hyperproduction and characterisation of cloned proteins - Principles and applications of the polymerase chain reaction
- Sequence analysis of informational macromolecules: basic procedures and projects
- Changing a genome, gene or gene-product: site-directed mutagenesis and protein engineering
Study material:
Books:
Old & Primrose, Principles of Gene Manipulation, 6th edition, available in summer 2001 Greene & Rao, Recombinant DNA Principles and Methodologies,
GGS (short)
Basic Principles of Gene and Protein Technology (G. Volckaert) Contents:
- RecDNA: enzymatic armamentorium and other tools for in vitro construction and analysis.
- Cloning vectors and basal techniques of DNA cloning, construction & analysis of DNA libraries.
- Expression strategies, hyperproduction and characterisation of cloned proteins.
- Principles and applications of the polymerase chain reaction.
- Sequence analysis of informational macromolecules.
- Changing a genome, gene or gene-product: site-directed mutagenesis and protein engineering.
Study material:
Books:
Old & Primrose, Principles of Gene Manipulation, 6th edition, available in summer 2001 Greene & Rao, Recombinant DNA Principles and Methodologies,
