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Exploring the fungal wall proteome by mass spectrometry
Yin, Q.Y.
Publication date
2008
Link to publication
Citation for published version (APA):
Yin, Q. Y. (2008). Exploring the fungal wall proteome by mass spectrometry. Digital Printing
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ABBREVIATIONS
2-DE Two-dimensional electrophoresis
amu Atomic mass unit
ASL Alkali-sensitive linkage
BCA Bicinchoninic acid
BLAST Basic local alignment search tool BPI Base peak intensity
CAI Codon adaptation index
CBM Carbohydrate-binding module
CFEM (a protein domain) common in fungal extracellular membrane/wall proteins
CFW Calcofluor white
CID Collision-induced dissociation CWI Cell wall integrity
CWP Cell wall protein Da Dalton
ESI Electrospray ionization FBS Fetal bovine serum
GFP Green fluorescent protein
GH Glycoside hydrolase
GlcNAc N-Acetylglucosamine
GPI Glycosylphosphatidylinositol
GPI-CWP Glycosylphosphatidylinositol-modified cell wall protein HPLC High performance liquid chromatography
ICAT Isotope-coded affinity tags
iTRAQ Isobaric relative and absolute quantitation
LC Liquid chromatography
LC-MS/MS Liquid chromatography coupled to tandem mass spectrometry via electrospray
MALDI Matrix-assisted laser desorption/ionization
αMEM Eagle’s minimum essential medium with alpha modification mRNA Messenger ribonucleic acid
MS Mass spectrometry
MS/MS Tandem mass spectrometry
m/z Mass over charge ratio OD Optical density
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PAGE Polyacrylamide gel electrophoresis Pir Protein with internal repeats
QTOF Quadrupole and time-of-flight hybrid mass spectrometer SCMD Saccharomyces cerevisiae Morphological Database
SDS Sodium dodecyl sulfate SGD Saccharomyces Genome Database
SILAC Stable isotope labeling with amino acids in cell culture TIC Total ion current
TOF Time-of-flight mass spectrometer UV Ultra-violet
