Cover Page
The handle
http://hdl.handle.net/1887/123040
holds various files of this Leiden University
dissertation.
Author: Kytidou, K.
Title: Transfer of goods from plants to humans: Fundamental and applied biochemical
investigations on retaining glycosidases
Acknowledg
ements
I have so many people to thank for completing this work. First, I am grateful to my supervisor Hans Aerts. Hans, your precious guidance, valuable feedback and inspiration have shown me the true meaning of the word ‘professor’. A special thanks also to my family, friends and colleagues, who’s tremendous (moral) support, encouragement and unconditional love I will always cherish. Some claim that poets can express everything through their art. Thus, I would like to dedicate the poem Ithaka, written by a (non-ancient) Greek poet, Konstantinos P. Kavafis in 1911, to everyone that supported and helped me over this PhD “journey”.
Ithaka
As you set out for Ithaka hope your road is a long one, full of adventure, full of discovery.
Laistrygonians, Cyclops, angry Poseidon—don’t be afraid of them: you’ll never find things like that on your way
as long as you keep your thoughts raised high, as long as a rare excitement
stirs your spirit and your body. … …. Hope your road is a long one. May there be many summer mornings when,
so, you’re old by the time you reach the island, wealthy with all you’ve gained on the way,
not expecting Ithaka to make you rich. Ithaka gave you the marvellous journey.
Without her you wouldn’t have set out. She has nothing left to give you now.
And if you find her poor, Ithaka won’t have fooled you. Wise as you will have become, so full of experience, you’ll have understood by then what these Ithakas mean.
Curriculum Vitae
1 March 1989
2003-2006
2006-2011
2012-2014
2014-2019
Born in Thessaloniki, Greece
3
rdGeneral Lyceum of
Thessaloniki, Greece
Bachelor of Science, Molecular
Biology and Genetics.
Dimokrition University of Thrace,
Alexandroupolis, Greece
Publications
Kytidou K., Beekwilder J., Artola M., Meel E. van, Wilbers R.H.P., Moolenaar G.F., Goosen N., Ferraz M.J., Katzy R., Voskamp P., Florea B.I., Hokke C.H., Overkleeft H.S., Schots A., Bosch D., Pannu N. & Aerts J.M.F.G. (2018), Nicotiana benthamiana α-galactosidase A1.1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease, Journal of Biological Chemistry 293(26): 10042-10058.
Kytidou K., Beenakker T.J.M., Westerhof L.B., Hokke C.H., Moolenaar G.F., Goosen N., Mirzaian M., Guimaraes Da Lomba Ferraz M.J., Geus M.A.R. de, Kallemeijn W.W., Overkleeft H.S., Boot R.G., Schots A., Bosch D. & Aerts J.M.F.G. (2017), Human Alpha Galactosidases Transiently Produced in Nicotiana benthamiana Leaves: New Insights in Substrate Specificities with Relevance for Fabry Disease, Frontiers in Plant Science 8: 1026.
Kytidou K., Artola M., Overkleeft, H.S., & Aerts J.M.F.G. (2020), Plant Glycosides and Glycosidases: A Treasure-Trove for Therapeutics, Frontiers in Plant Science, 11: 357.
Kuo C.L., Meel H.M.M. van, Kytidou K., Kallemeijn W.W., Witte M., Overkleeft H.S., Artola Pérez de Azanza M.E. & Aerts J.M.F.G. (2018), Activity-Based Probes for Glycosidases: Profiling and Other Applications. In: Imperiali B. (Ed.) Chemical Glycobiology Part B. Monitoring Glycans and their Interactions. Methods in Enzymology no. 598: Elsevier: Monograph Series. 217-235.
Artola M., Hedberg C., Rowland R.J., Raich L., Kytidou K., Wu L., Schaaf A., Ferraz M.J., Marel G.A. van der, Codee J.D.C., Rovira C., Aerts J.M.F.G., Davies G.J. & Overkleeft H.S. (2019), Alpha-d-Gal-cyclophellitol cyclosulfamidate is a Michaelis complex analog that stabilizes therapeutic lysosomal alpha-galactosidase A in Fabry disease, Chemical Science 10(40): 9233-9243.
Mirzaian M., Wisse P., Guimaraes da Lomba Ferraz M.J., Marques A.R., Gaspar P., Oussoren S.V., Kytidou K., Codee J.D.C., Marel G.A. van der, Overkleeft H.S. & Aerts J.M.F.G. (2017), Simultaneous quantitation of sphingoid bases by UPLC-ESI-MS/MS with identical C-13-encoded internal standards, Clinica Chimica Acta 466: 178-184.
Kytidou K., Denysiuk I., Kuo C.L., Artola M., Overkleeft H.S., Aerts J.M.F.G. Diagnosis with activity-based probes of inherited glycosidase deficiencies using urine samples. To be submitted.
