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University of Groningen Lactococcus lactis bacteriophages: phage-host interaction and phage transduction Marcelli, Barbara

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University of Groningen

Lactococcus lactis bacteriophages: phage-host interaction and phage transduction

Marcelli, Barbara

DOI:

10.33612/diss.118088081

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2020

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Marcelli, B. (2020). Lactococcus lactis bacteriophages: phage-host interaction and phage transduction. Rijksuniversiteit Groningen. https://doi.org/10.33612/diss.118088081

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Lactococcus lactis

bacteriophages:

phage-host interaction and

phage transduction

Barbara Marcelli

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The work described in this thesis was performed at the Department of Molecular Genetics, Groningen Institute for Molecular Biology and Biotechnology, Faculty of Science and Engineering, University of Groningen, The Netherlands. The research was carried out within the BE-Basic R&D Program, which was granted an FES subsidy from the Dutch Ministry of Economic Affairs.

Printing of this thesis was financially supported by the Graduate School of Science and the University of Groningen.

Cover design: Emanuela Gigliotti Thesis design: Douwe Oppewal

Printed by: Ipskamp Drukkers B.V., Enschede, The Netherlands. ISBN E-book: 978-94-034-2478-1

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Lactococcus lactis bacteriophages:

phage-host interaction and

phage transduction

PhD thesis

to obtain the degree of Phd at the

University of Groningen

on the authority of the

Rector Magnificus Prof. C. Wijmenga

and in accordance with

the decision by the College of Deans.

This thesis will be defended in public on

Friday 13 March 2020 at 14.30 hours

By

Barbara Marcelli

Born on 4 March 1989

In Rome, Italy

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Supervisors

Prof. O.P. Kuipers Prof. J. Kok

Assessment committee

Prof. D. Van Sinderen Prof. J.D. Van Elsas Prof. L. Dijkhuizen

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CONTENTS

Chapter 1 General introduction 9

Chapter 2 Analysing the genetic determinants of the host-range of 29 22 newly identified lactococcal bacteriophages

Chapter 3 The feasibility of phage-mediated horizontal gene transfer in 55

Lactococcus lactis

Chapter 4 A specific sugar moiety in the Lactococcus lactis cell wall pellicle 83

is required for infection by CHPC971, a member of the rare 1706 phage species

Chapter 5 Alternative phage-host interaction in Lactococcus lactis: 115 the carrier state drives rapid evolution of phages

Chapter 6 Complete genome sequence of 28 lactococcal bacteriophages 145 isolated from failed dairy fermentation processes

Chapter 7 Summary and general discussion 151

Nederlandse samenvatting 161

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