University of Groningen
Mass spectrometry-based methods for protein biomarker quantification
Klont, Frank
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Publication date: 2019
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Klont, F. (2019). Mass spectrometry-based methods for protein biomarker quantification: On the road to clinical implementation. University of Groningen.
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Propositions
1. A protein biomarker needs a proper test like a drug needs a proper formulation. (this thesis)
2. Different quantitative methods targeting the same analyte should be considered as being noninterchangeable unless proven otherwise. (this thesis)
3. MALDI-TOF mass spectrometry could be a suitable technique for quantifying small protein biomarkers like insulin-like growth factor 1, IGF1, provided that the target of interest is adequately enriched from the sample matrix and that an appropriate internal standard is used. (this thesis)
4. Adsorptive microtiter plates may represent a convenient alternative to magnetic beads as solid support in affinity purification workflows targeting low abundant proteins such as the soluble receptor of advanced glycation end-products, sRAGE. (this thesis)
5. Affimers are small and versatile affinity ligands with significant potential for biomedical applications, notably by serving as robust alternatives to antibodies. (this thesis)
6. Do not rely on the average, calculated physicochemical properties of a protein but also study these properties for its distinct functional and structural domains separately when attempting to establish sample preparation methods on the basis of a rational design. (this thesis)
7. No method in discovery-based proteomics will perform best for all types of samples or experiments, hence the full potential of a proteomics experiment may not be reached if the same method is used all the time. (this thesis)
8. 100% of me is nothing compared to 1% of the whole team. (Eliud Kipchoge) 9. Positive reinforcement changes behavior for the better, while criticism stabilizes
negative behaviors and blocks change. (Virginia H. Pearce)
10. Es ist nicht genug zu wissen, man muß auch anwenden. Es ist nicht genug zu wollen, man muß auch tun. (Johann Wolfgang von Goethe)
11. Angst is mar veur eben, spiet is veur altied. (Daniël Lohues)
