University of Groningen
Assembly dynamics of supramolecular protein-DNA complexes studied by single-molecule
fluorescence microscopy
Stratmann, Sarah
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Publication date:
2017
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Stratmann, S. (2017). Assembly dynamics of supramolecular protein-DNA complexes studied by
single-molecule fluorescence microscopy. Rijksuniversiteit Groningen.
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Stellingen
behorende bij het proefschrift
Assembly dynamics of supramolecular protein-DNA complexes
studied by single-molecule fluorescence microscopy
Sarah Stratmann
1. Biochemical pathways within the cellular interior are shaped by the heterogeneity of molecular populations. (Chapter 2 of this thesis)
2. While the enzymes acting on the leading and lagging strand such as polymerases, clamp loader complexes and primases frequently dissociate and exchange with proteins in solution, DnaB ensures replisome integrity by providing a stable interaction platform. (Chapter 3 of this thesis)
3. “It seems paradoxical that DnaC inhibits the function of a complex whose formation it promotes. This dual action may reflect a feedback control mechanism.” George Allen and Arthur Kornberg, 1991
4. 1D-diffusion mediated assembly plays a key role in regulating the overall IFI16-mediated immune responses upon pathogen detection. (Chapter 4 of this thesis) 5. In a typical surface-based single molecule experiment, a DNA-binding protein
complex is likely to aggregate not only on the DNA substrate, but also on the coverglass surface, leading to potential artifacts in the experimental outcome. (Chapter 5 of this thesis)
6. “Aunque sólo existiera una verdad única, no se podrían pintar cien cuadros sobre el mismo tema.” – “If there were only one truth, you couldn't paint a hundred canvases on the same theme.” Pablo Picasso
7. Although for a lab supplier discontinuing a product is perfectly sensible, it might have unforeseen impact on the life of a very small percentage of academic researchers.
8. „Alle Naturwissenschaften geben uns Antwort auf die Frage: Was sollen wir tun,
wenn wir das Leben technisch beherrschen wollen? Ob wir es aber technisch beherrschen sollen und wollen, und ob das letztlich eigentlich Sinn hat: – das lassen sie ganz dahingestellt oder setzen es für ihre Zwecke voraus.“ – “Natural science gives us an answer to the question of what we must do if we wish to master life technically. It leaves quite aside, or assumes for its purposes, whether we should and do wish to master life technically and whether it ultimately makes sense to do so.” Max Weber, Wissenschaft als Beruf, 1919
