Development of a vernix caseosa substitute : a novel strategy to improve skin barrier function and repair
Rißmann, R.
Citation
Rißmann, R. (2009, March 17). Development of a vernix caseosa substitute : a novel strategy to improve skin barrier function and repair. Retrieved from https://hdl.handle.net/1887/13664
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Development of a vernix caseosa substitute
A novel strategy to improve skin barrier function and repair
Robert Rißmann
Development of a vernix caseosa substitute
A novel strategy to improve skin barrier function and repair Robert Rißmann
PhD thesis with summary in Dutch March 2009
ISBN: 978-90-9023896-8
©2009 Robert Rißmann. All rights reserved. No part of this thesis may be reproduced or transmitted in any form or by any means without written permission of the author.
Cover: Drawing by Leonardo da Vinci, design by Hagen Rißmann Printed by Wöhrmann Print Service, Zutphen, The Netherlands
Development of a vernix caseosa substitute
A novel strategy to improve skin barrier function and repair
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus prof.mr. P.F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op dinsdag 17 maart 2009 klokke 16.15 uur
door
Robert Rißmann geboren te Berlijn, Duitsland
in 1977
Promotiecommissie
Promotoren: Prof. Dr. J.A. Bouwstra
Prof. Dr. W.E. Hennink (Universiteit Utrecht) Copromotor: Dr. M. Ponec
Referent: Dr. A.V. Rawlings (AVR Consulting, UK)
Overige leden: Prof. Dr. M. Danhof
Prof. Dr. H.W. Frijlink (Universiteit Groningen)
Prof. Dr. W. Jiskoot
Dr. S. Pavel
The investigations described in this thesis were performed at the Division of Drug Delivery Technology of the Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.
The studies were financed by the Dutch Technology Foundation (STW LGT6117).
The publication of the thesis was financially supported by the Dutch Technology Foundation, the Leiden/Amsterdam Center for Drug Research and Croda Europe Ltd (UK).
Table of Contents
Chapter 1 - General introduction 7
Part I – Detailed characterization of vernix caseosa (lipids)
Chapter 2 - New Insights into ultrastructure, lipid composition
and organization of vernix caseosa 27
Chapter 3 - Temperature-induced changes in structural and physicochemical properties of vernix caseosa 49
Chapter 4 - Long periodicity phase in extracted lipids of vernix caseosa obtained with equilibration at physiological temperature 67
Part II – Mimicking vernix caseosa (lipids) Chapter 5 - Lanolin-derived lipid mixtures mimic closely the composition and organization of vernix caseosa lipids 87
Chapter 6 - Mimicking vernix caseosa - preparation and characterization of synthetic biofilms 111
Part III – In vivo studies to test biological efficacy of natural and synthetic biofilm(s) Chapter 7 - Development of a murine model to evaluate the effect of vernix caseosa on skin barrier recovery 129
Chapter 8 - Skin barrier disruption by acetone – observations in a hairless mouse model 147
Chapter 9 - Effect of synthetic vernix biofilms on barrier recovery of damaged mouse skin 159
Chapter 10 - Summary and perspectives 181
Appendix Samenvatting (Dutch Summary) 195
List of abbreviations 203
List of publications 205
Curriculum Vitae 207
Acknowledgements 209
