DNA damage signaling networks: from stem cells to cancer
Carreras Puigvert, J.
Citation
Carreras Puigvert, J. (2011, October 20). DNA damage signaling networks:
from stem cells to cancer. Retrieved from https://hdl.handle.net/1887/17980
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/17980
Note: To cite this publication please use the final published version (if applicable).
DNA damage signaling networks:
from stem cells to cancer
Jordi Carreras Puigvert
DNA damage signaling networks: from stem cells to cancer Jordi Carreras Puigvert
October 2011
Cover: HepG2 cell with Annexin V staining, by Lisa Fredriksson and Bram Herpers.
Printed by Wöhrmann Print Service, Zutphen, The Netherlands ISBN: 978-90-8570-427-0
2011, Jordi Carreras Puigvert. All rigths reserved. No part of this thesis my be reproduced or transmitted in any form, by any means, electronic or mechanical, without prior written permission of the author.
DNA damage signaling networks:
from stem cells to cancer
Proefschrift ter verkrijging van
de graad van Doctor aan de Universiteit van Leiden, op gezag van Rector Magnificus prof. mr. P.F. van der Heijden,
volgens besluit van het College voor Promoties te verdedigen op donderdag 20 Oktober 2011
klokke 16.15 uur
door
Jordi Carreras Puigvert geboren te La Roca del Vallès
in 1978
Promotion Committee
Promotor:
Prof. Dr . B. van de Water LACDR, Leiden
Co-promotor:
Dr. E.H.J. Danen LACDR, Leiden
Overige leden:
Prof. Dr. J. Kuiper LACDR, Leiden Prof. Dr. J. Brouwer LACDR, Leiden Prof. Dr. M. Danhof LACDR, Leiden Prof. Dr. L.H.F. Mullenders LUMC, Leiden
Prof. Dr. G.T.J. van der Horst Erasmus MC, Rotterdam Dr. W. Vermeulen Erasmus MC, Rotterdam Prof. Dr. H.P.J. Te Riele NKI, Amsterdam
The investigations described in this thesis were performed at the Division of Toxicology of the Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.
The printing of this thesis was financially supported by:
Leiden/Amsterdam Center for Drug Research J.E. Jurriaanse Stichting.
A la meva mare
Table of contents
Chapter 1 9 General introduction and scope of this thesis.
Chapter 2 27 The cancer stem cell microenvironment and anti-cancer therapy.
International Journal of Radiation Biology
Chapter 3 37 High throughput live cell imaging of apoptosis.
Current Protocols in Cell Biology
Chapter 4 53 Global phosphoproteome profiling reveals unanticipated
networks responsive to cisplatin treatment of embryonic stem cells.
Molecular and Cellular Biology, under revision
Chapter 5 87 Mapping DNA damage response signaling networks in ES
cells - downregulation of CSNK1a1 leads to enhanced Wnt signaling that acts as a brake on p53-mediated apoptosis.
Manuscript in preparation
Chapter 6 117 Integrins and oncogenes: partners in crime.
Molecular and Cellular Pharmacology
Chapter 7 125 Cross-talk between integrins and oncogenes modulates chemosensitivity.
Molecular Pharmacology
Chapter 8 137 Summary and discussion
Appendix:
Nederlandse samenvatting 147
Resum en Català 153
Curriculum vitae 157
List of publications 159
Acknowledgments 161
