Diagnostics of non-tuberculous mycobacteria
Bruijnesteijn van Coppenraet, L.E.S.
Citation
Bruijnesteijn van Coppenraet, L. E. S. (2009, March 5). Diagnostics of non-tuberculous mycobacteria. Retrieved from https://hdl.handle.net/1887/13665
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/13665
Note: To cite this publication please use the final published version (if applicable).
Diagnostics of Non-Tuberculous Mycobacteria
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus prof.mr. P.F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op donderdag 5 maart 2009 klokke 16.15 uur
door
Lesla E. S. Bruijnesteijn van Coppenraet
geboren te Arnhem
in 1976
Promotiecommissie
Promotor Prof. Dr. A.C.M. Kroes Copromotor Dr. E.J. Kuijper
Referent Dr. A.H.J. Kolk (Koninklijk Instituut voor de Tropen, Amsterdam) Overige leden Prof. Dr. J.T. van Dissel
Prof. Dr. J.P.R. van Merkesteyn
Prof. Dr. P.H.M. Savelkoul (Vrije Universiteit Amsterdam)
Van dit proefschrift is ook een handelseditie verschenen bij Uitgeverij BOX Press te Oisterwijk onder ISBN9789088910920
Financial support for the printing of this thesis was kindly provided by:
BioMérieux, Pathofinder,
Nederlandse Vereniging voor Medische Microbiologie,
Afd. Medische Microbiologie, Leids Universiteit Medisch Centrum,
Afd. Medische Microbiologie en Infectieziektenpreventie, Isala Klinieken Zwolle
Illustrations cover: Bodymention Leiderdorp
© L.E.S. Bruijnesteijn van Coppenraet, 2009
Table of Contents.
1. Introduction.
a. General introduction.
b. Taxonomy of the genus Mycobacterium.
c. Disease caused by NTM.
i. Pulmonary infections.
ii. Skin infections.
iii. Lymphadenitis.
iv. Disseminated disease.
v. Skeletal infections.
vi. Gastrointestinal infections.
vii. Foreign body related and nosocomial infections.
d. Natural reservoirs.
e. Pathogenesis and host defences.
f. Treatment of NTM.
2. Diagnosis of NTM disease.
a. Clinical diagnosis.
b. Immunological tests.
c. Histopathological diagnosis.
d. Conventional microbiological diagnosis.
i. Acid-fast staining.
ii. Decontamination.
iii. Culture.
iv. Species identification.
e. Molecular identification of NTM species.
f. Molecular detection of NTM in clinical specimens.
g. Susceptibility testing.
i. Conventional methods.
ii. Molecular approaches for susceptibility testing.
h. Typing of NTM.
i. Conclusions and design of the thesis.
3. Real-time PCR assay using fine-needle aspirates and tissue
biopsyspecimens for rapid diagnosis of mycobacterial lymphadenitis in children.J Clin Microbiol. 2004;42:2644-50.
4. Mycobacterium haemophilum and lymphadenitis in children. Emerg Infect Dis. 2005;11:62-8.
5. Application of real-time PCR to recognize atypical mycobacteria in archival skin biopsies: high prevalence of Mycobacterium
haemophilum. Diagn Mol Pathol. 2007;16:81-6.
5
23
57
73
85
3
6. Amplified Fragment Length Polymorphism (AFLP) analysis of human clinical isolates of Mycobacterium haemophilum from different continents. Clin Microbiol and Infect. Accepted November 2008.
7. Lymphadenitis in children is caused by Mycobacterium avium
hominissuis and not related to 'bird-tuberculosis'. Eur J Clin Microbiol Infect Dis. 2008;27:293-9.
8. Comparison of different genes for the identification of atypical mycobacteria by DNA sequencing.
9. Discussion: Diagnostics of non-tuberculous mycobacteria.
a. Introduction.
b. Molecular identification of species and recognition of subspecies.
c. Real-time PCR for the detection of NTM in clinical materials.
d. Suggestions to improve molecular detection of NTM in clinical materials.
i. Increasing the sensitivity.
ii. Optimization for molecular detection in pathological formalin fixed-paraffin embedded materials.
iii. Prevention of contamination.
e. Susceptibility.
f. Typing of NTM for taxonomic purpose and as an epidemiological tool.
i. A new typing method: Protein profiling.
g. General conclusion.
Samenvatting (Dutch Summary).
Curriculum Vitae.
Publicaties.
97
111
123
135
149
153
154
4
