University of Groningen
Liposome interactions with biological systems: a journey into cells
Yang, Keni
DOI:
10.33612/diss.123825197
IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.
Document Version
Publisher's PDF, also known as Version of record
Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Yang, K. (2020). Liposome interactions with biological systems: a journey into cells. University of Groningen. https://doi.org/10.33612/diss.123825197
Copyright
Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).
Take-down policy
If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.
Liposome Interactions with
Biological Systems: A
Journey into Cells
Keni Yang
The work described in this Thesis was performed in the department of
Pharmacokinetics, Toxicology and Targeting at the Research Institute of Pharmacy
(GRIP), University of Groningen (RUG), the Netherlands, and financially supported
by the China Scholarship Council (CSC).
Printing of this Thesis was financially supported by the University Library and the
Graduate School of Science, Faculty of Science and Engineering, University of
Groningen, the Netherlands.
Cover & Layout: Keni Yang
Printed by: proefschrift-aio
ISBN:
978-94-034-2589-4 (printed version)ISBN: 978-94-034-2588-7 (electronic version)
Copyright @ Keni Yang, Groningen, the Netherlands, 2020
All rights reserved. No part of this thesis ma y be reproduced in any form or by any
means without prior permission of the author.
Liposome Interactions with
Biological Systems: A Journey into
Cells
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Friday 8 May 2020 at 09.00 hours
by
Keni Yang
born on 27 November 1989
in Guangxi, China
Supervisors
Prof. A. Salvati
Prof. K. Poelstra
Assessment Committee
Prof. F. Stellacci
Prof. G. Storm
Prof. W.J. Quax
Cahpter 1:General Introduction and Scope of the Thesis ... 1
1. Nanomedicine ... 2
2. Biological challenges of nanomedicines for their clinical translation ... 4
3. Extracellular and intracellular nano-bio interactions... 6
4. Liposome targeting with endogenous components ... 18
5. Scope and outline of the Thesis ... 20
References ... 23
Chapter 2: Tuning Liposome Composition to Modulate Corona Formation in Human Serum and Cellular Uptake ... 31
ABSTRACT ... 32
1. Introduction ... 32
2. Materials and methods... 35
3. Results and discussion ... 42
4. Conclusions ... 58
Acknowledgments ... 60
References ... 61
Supplementary Materials ... 65
Chapter 3: Effects of Protein Source on Liposome Uptake by Cells: Corona Composition and Impact of the Excess Free Proteins ... 83
ABSTRACT ... 84
1. Introduction ... 84
2. Materials and methods... 86
3. Results and discussion ... 93
4. Conclusions ... 103
Acknowledgements ... 104
References ... 105
Supplementary Materials ... 109
Chapter 4: Comparison of the Uptake Mechanisms of Zwitterionic and Negatively Charged Liposomes by HeLa Cells... 113
ABSTRACT ... 114
1. Introduction ... 114
2. Materials and methods... 116
3. Results and discussion ... 120
Acknowledgments ... 129
References ... 130
Supplementary Materials ... 134
Chapter 5: Tuning Liposome Stability in Biological Environments and Intracellular Drug Release Kinetics ... 139
ABSTRACT ... 140
1. Introduction ... 140
2. Materials and methods... 142
3. Results and discussion ... 148
4. Conclusions ... 157
Acknowledgments ... 158
References ... 159
Supplementary Materials ... 162
Chapter 6: Cell Membrane Nanoparticles to Probe the Interactions between Stromal and Cancer Cells in Acute Myeloid Leukemia ... 167
ABSTRACT ... 168
1. Introduction ... 168
2. Materials and methods... 170
3. Results and discussion ... 177
4. Conclusions ... 186
Acknowledgements ... 187
References ... 189
Supplementary Materials ... 193
Chapter 7: General Discussion and Future Perspective ... 197
References ... 209
Nederlandse samenvatting ... 211
Acknowledgements ... 217
List of publications ... 223
Manuscripts submitted or in preparation ... 224
