University of Groningen
Lanthipeptide engineering: non-canonical amino acids, click chemistry and ring shuffling
Deng, Jingjing
DOI:
10.33612/diss.112973724
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Deng, J. (2020). Lanthipeptide engineering: non-canonical amino acids, click chemistry and ring shuffling. University of Groningen. https://doi.org/10.33612/diss.112973724
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Propositions
associated with the PhD thesis
Lanthipeptide engineering: non-canonical amino acids,
click chemistry and ring shuffling
By Jingjing Deng
1. Lantibiotics may one day be part of the antimicrobial arsenal to combat antimicrobial resistance. (This thesis)
2. NisP is a suitable and inexpensive protease for the activation of diverse heterologously expressed lantibiotics, produced with the nisin leader and the modification machinery of nisin. (Chapter 2)
3. Large-scale engineering of lanthipeptides is a promising strategy to obtain novel bioactive variants. (Chapter 3)
4. Incorporation of non-canonical amino acids into lantibiotics can dramat-ically expand their chemical and functional space and enables the design of novel lantibiotics with improved properties (e.g. stability, specificity orbioavailability). (Chapter 4)
5. The reactive groups of specific non-canonical amino acids (e.g. alkyne and azide) can serve as chemical handles for click chemistry to conjugate antimicrobial peptides with fluorophores, peptide moieties or other antimicrobial moieties. (Chapter 4 and Chapter 5)
6. Lantibiotics can be used as lead structures to create novel variants with altered properties via chemical coupling. (Chapter 5)
7. The purpose of education is to replace an empty mind with an open one. (Malcolm Forbes)
8. Covering ninety miles is still half way to a one-hundred-mile journey. (行百里者半九十) (Strategies of the warring states)
