University of Groningen
Adding tools to the box: facilitating host strain engineering of Penicillium chrysogenum for the
production of heterologous secondary metabolites
Pohl, Carsten
DOI:
10.33612/diss.119054818
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Publication date:
2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Pohl, C. (2020). Adding tools to the box: facilitating host strain engineering of Penicillium chrysogenum for
the production of heterologous secondary metabolites. University of Groningen.
https://doi.org/10.33612/diss.119054818
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Stellingen
Adding tools to the box:
facilitating host strain engineering of
Penicillium chrysogenum
for the production of
heterologous secondary metabolites
Carsten Pohl
1. Implementation of the CRISPR/Cas9 toolbox presented is expected to lead to an acceleration in the discovery and activation of novel natural products. The prospect of bringing these tools to non-domesticated fungal species opens novel paths for natural product discovery and exploitation. – this Thesis
2. Ideas alone have little worth. The value of an innovation lies in its practical implementation. - Werner von Siemens
3. Recent developments in the fields of synthetic biology and DNA synthesis are rapidly improving our capability to engineer new functionalities into microorganisms. – this Thesis 4. A heterologous expression strategy should be able to accommodate quick changes in
DNA constructs tested, preferably by direct integration of multiple DNA parts that can be redistributed or replaced by modified parts in a design-build-test-learn cycle. – this Thesis 5. The calbistrin cluster potentially encodes many of the predicted required enzymatic
activity required for de novo synthesis of calbistrin, but the mechanism of synthesis of the linear moiety remains a challenge. – this Thesis
6. A recent review lists 22 inducible promoters for fungi, yet the comparison of performance is not possible as the read-out for promoter performance is not uniform. – Kluge et al., 2018 7. Our approach to skip the process of single-colony isolation and verification by testing
pooled clones is very appealing for rapid direct gain-of-function analysis of newly designed pathways in fungal hosts. – this Thesis
8. I cannot chase you, only motivate you. The best is that it comes from within yourself. At any rate, you are heading the right direction. – Arnold Driessen
9. I have always struggled to achieve excellence. One thing that cycling has taught me is that if you can achieve something without a struggle it’s not going to be satisfying – Gregory
