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Evolution of Viola stagnina and its sisterspecies by hybridisation and polyploidisation Hof, K. van den

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Hof, K. van den. (2010, June 9). Evolution of Viola stagnina and its sisterspecies by hybridisation and polyploidisation. Retrieved from https://hdl.handle.net/1887/15684

Version: Corrected Publisher’s Version

License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden

Downloaded from: https://hdl.handle.net/1887/15684

Note: To cite this publication please use the final published version (if applicable).

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Evolution of Viola stagnina and its Sisterspecies by

Hybridization and Polyploidization

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gevoel. Ze symboliseren nederigheid en trouw.

Kevin van den Hof

Evolution of Viola stagnina and its Sisterspecies by Hybridization and Polyploi- dization

Cover design and

lay-out : René Glas (www.reneglas.com) Drawings, fig. 9 : Esmée Winkel

Photograph, fig.17 : Bertie-Joan van Heuven Printed by : Offsetdrukkerij Nautilus, Leiden

Chapter 2 : Published in Molecular Biology and Evolution 25:

2099–2108 (2008). van den Hof K, van den Berg RG, Gravendeel B. Chalcone synthase gene lineage diversifica tion confirms allopolyploid evolutionary relationships of European rostrate violets.

Chapter 3 : Submitted to Taxon Chapter 4 : Submitted to Taxon

Chapter 5 : Submitted to Conservation Genetics Chapter 6 : Submitted to Plant Ecology and Evolution

The remainder of the thesis ©2010, Netherlands Centre for Biodiversity Naturalis (section NHN), Leiden University.

No part of this publication, apart from bibliographic data and brief annotations in critical reviews, may be reproduced, re-recorded or published in any form, including print, photocopy, microform, electronic or electromagnetic record without written permission by the publishers.

ISBN/EAN: 978-90-71236-71-6

Dedicated to the memory of

R u u d va n d e r M e i j d e n

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Evolution of Viola stagnina and its Sisterspecies by Hybridization and Polyploidization

Proefschrift

ter verkrijging van de graad van Doctor aan de Universiteit Leiden,

op gezag van de Rector Magnificus prof. mr. P.F. van der Heijden, volgens het besluit van het College van Promoties

te verdedigen op woensdag 9 juni 2010 klokke 16:15 uur

door

Kevin van den Hof

Geboren te Geleen in 1980

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Copromotor • Dr. B. Gravendeel Dr. R.G. van den Berg

Referent • Prof. Dr. H.E. Ballard Jr.

Overige leden • Prof. Dr. P. Baas Dr. T. Marcussen Dr. M.E. Noordeloos Prof. Dr. M. Schilthuizen

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C o n t e n t s

Chapter 1 • General introduction 7

Chapter 2 • Chalcone Synthase Gene Lineage Diversification confirms Al- lopolyploid Evolutionary Relationships of European Rostrate Violets

13

Chapter 3 • Viola montana and V. persicifolia (Violaceae): two names to be rejected

27

Chapter 4 • Proposal to reject the names Viola montana and V. persicifolia (Violaceae)

41

Chapter 5 • Combined analyses of AFLP markers and morphology confirm the taxonomic status of Viola stagnina var. lacteoides

45

Chapter 6 • Phenotypic plasticity of Viola stagnina 65

Chapter 7 • Summary & Conclusion 75

Nederlandse samenvatting 79

References 83

Appendix I • Voucher information of taxa sampled 95

Appendix II • List of species and accessions used for AFLP analyses 99

Appendix III• List of characters used for morphometric analyses 105

Curriculum Vitae 109

Nawoord 111

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